DiO/DPA Membrane potential detection kit stain for histology
Supplier: Biotium
The DiO/DPA system detects cytoplasmic membrane potential changes using the principle of fluorescence resonance energy transfer (FRET), where DiO is a “stationary” FRET donor while DPA acts as a potentiometric FRET acceptor. The DiO/DPA system has been reported to produce a fluorescence signal change of >56% in HEK-293 cells and >25% in neuronal cultures and brain slices per 100 mV membrane potential change.
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