You searched for: Extraction Buffers

Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.

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HEPES lysis buffer with NP-40 (2X)

Supplier: Thermo Fisher Scientific

HEPES lysis buffer with NP-40 (2X), Thermo Fisher Scientific

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Protein extraction reagent, BugBuster® HT, Millipore®

Supplier: Merck Millipore (Novagen)

BugBuster® HT Protein Extraction Reagent combines BugBuster® Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster® HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from E. coli without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.

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MES lysis buffer with Triton® X-100

Supplier: Thermo Fisher Scientific

MES lysis buffer with Triton® X-100, Thermo Fisher Scientific

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NP-40 lysis buffer (2X)

Supplier: Thermo Fisher Scientific

NP-40 lysis buffer (2X), Thermo Fisher Scientific

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PIPES lysis buffer with NP-40 (2X)

Supplier: Thermo Fisher Scientific

PIPES lysis buffer with NP-40 (2X), Thermo Fisher Scientific

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Lysis buffers, red blood cells

Supplier: G-Biosciences

These DNase- and RNase-free buffers and reagents are designed for high quality research.

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Succinate, 0,2 M buffer solution, pH 5,0

Supplier: Thermo Fisher Scientific

Succinate, 0.2M buffer soln., pH 5.0, Thermo Fisher Scientific

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Triton® X-100 lysis buffer with glycerol (2X), pH 7,4

Supplier: Thermo Fisher Scientific

Triton® X-100 lysis buffer with glycerol (2X), pH 7.4, Thermo Fisher Scientific

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ACK Lysing Buffer (1x), TheraPEAK™

Supplier: LONZA

Ammonium-Chloride-Potassium (ACK) Lysing Buffer is used to lyse red blood cells in preparations containing white blood cells.

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CSPL buffer

Supplier: OMEGA BIO-TEK

Component of the Mag-Bind® plant DNA 96 plus kit.

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Glucose hemolysing solution

Supplier: DIAGNOSTIC SYSTEMS DIASYS

For use with glucose Gluc-DH FS and glucose Gluc-DH FSC.

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Buffers for Gel PCR Clean-up Kit, NucleoSpin®

Supplier: MACHEREY-NAGEL

Buffer, NTB, for clean up of SDS-containing samples, 150 ml, Macherey-Nagel

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Ammonium Chloride Solution

Supplier: STEMCELL Technologies

Ammonium chloride solution is recommended for the lysis of red blood cells (RBCs) in preparations of human and mouse peripheral blood, spleen or bone marrow cells. It is buffered and optimised for gentle lysis of erythrocytes, with minimal effect on leukocytes. It does not contain a fixative agent, therefore leukocytes are viable following RBC lysis.

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EasySep™ Red Blood Cell Lysis Buffer

Supplier: STEMCELL Technologies

EasySep™ Red blood cell lysis buffer is for use as directed with EasySep™ or RoboSep™ whole blood kits for the lysis of red blood cells prior to separation from whole blood.