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31 results for Assay Buffers

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Assay Buffers

Assay buffers are mixtures of buffers, salts, detergents, protein, preservatives, and coloring agents. These buffers are used in ELISA assays for dilution of standards, samples, and reagents, and for blocking of non-specific interactions. Buffers may be used individually or in combination, Buffers may be used individually or in combination. Blocking buffers are specifically designed to preserve the conformational spatial arrangement of proteins in their native folded state, ensuring the biological activity of the protein.

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J.T.Baker®, Sheath fluid, 3000/3500

Supplier: Avantor

Sheath fluid 3000/3500, J.T.Baker®

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J.T.Baker®, Sheath fluid, 3200/3400

Supplier: Avantor

Sheath fluid, 3200/4000, J.T.Baker®

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MMP-3 fluorescent assay buffer

Supplier: ENZO LIFE SCIENCES

MMP-3 Fluorescent assay buffer

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MMP-1 assay buffer

Supplier: ENZO LIFE SCIENCES

MMP-1 assay buffer

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SIRT assay buffer

Supplier: ENZO LIFE SCIENCES

SIRT assay buffer

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PTP1B 2x assay buffer

Supplier: ENZO LIFE SCIENCES

PTP1B 2x assay buffer

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Proteasome assay buffer

Supplier: ENZO LIFE SCIENCES

Assay buffer from the 20S Proteasome Assay Kit for Drug Discovery.

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HDAC assay buffer

Supplier: ENZO LIFE SCIENCES

HDAC assay buffer

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MMP-1 colorimetric assay buffer

Supplier: ENZO LIFE SCIENCES

MMP-1 colorimetric assay buffer

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Cathepsin S Assay Buffer

Supplier: ENZO LIFE SCIENCES

50mM potassium phosphate buffer, pH 6,5, 50 mM NaCl, 2 mM EDTA, 0,01% Triton X-100®, 0,5 mM DTT.

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Caspase assay buffer

Supplier: ENZO LIFE SCIENCES

50 mM HEPES, pH 7,4, 100 mM NaCl, 0,1% CHAPS, 10 mM DTT, 1 mM EDTA, 10% glycerol.

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HDAC assay buffer II

Supplier: ENZO LIFE SCIENCES

50 mM Tris/Cl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 1 mg/ml BSA.

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Tween-20 solution (10%)

Supplier: ENZO LIFE SCIENCES

This Tween®-20 solution (10%) is specially designed to create the Blocking Buffer and Assay Buffer in ImmunoSet products. Each bottle is sufficient for use with 5×96 wells microtiter plates if following the instructions in our ImmunoSet protocol.

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Permeabilisation, (buffer)

Supplier: ENZO LIFE SCIENCES

Permeabilization, (buffer)

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Fixation/Permeabilisation, (buffer)

Supplier: ENZO LIFE SCIENCES

Fixation/Permeabilization, (buffer)

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Wash buffer concentrate

Supplier: ENZO LIFE SCIENCES

Liquid. TRIS buffered saline containing detergents.

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Background suppressor system (Permeabilising), TrueBlack® IF

Background suppressor system (Permeabilising), TrueBlack® IF

Supplier: Biotium

A buffer set that blocks non-specific antibody binding as well as direct interaction of fluorescent dyes on antibodies with cells or tissue sections.

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IHC PBS tablets

Supplier: ENZO LIFE SCIENCES

Solid. 100 ml PBS (1X) tablets containing 137 mM sodium chloride, 2.7 mM potassium chloride, and 10 mM phosphate buffer.

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Fixation, (buffer)

Supplier: ENZO LIFE SCIENCES

Fixation, (buffer)

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Buffers, potassium phosphate buffer (0,5 mol/L) solutions

Supplier: Cayman Chemical

Potassium phosphate buffer solution (0,5 mol/l), pH value: 8,0

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Buffers, sodium phosphate buffer solutions (0,5 mol/L)

Supplier: Cayman Chemical

Sodium phosphate stock solution (0,5 mol/L), pH value: 9,0

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ReadiUse™ Annexin Binding Buffer, Optimised for Imaging and Flow Cytometry

ReadiUse™ Annexin Binding Buffer, Optimised for Imaging and Flow Cytometry

Supplier: AAT BIOQUEST

The ReadiUse™ Annexin Binding Buffer *Optimised for Imaging and Flow Cytometry* is an isotonic buffer designed to promote the labeling of apoptotic cells with annexin V conjugates.

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Buffer, alkali solution (3,0 N)

Buffer, alkali solution (3,0 N)

Supplier: Hach

These buffers are used in the determination of calcium and magnesium in brine.

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ReadiUse™ Tyramide (TSA)/Styramide (PSA) Optimized Reaction Buffer (1000 Reactions)

ReadiUse™ Tyramide (TSA)/Styramide (PSA) Optimized Reaction Buffer (1000 Reactions)

Supplier: AAT BIOQUEST

For many immunohistochemical (IHC) applications, the traditional enzymatic amplification procedures are sufficient for achieving adequate antigen detection.

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ReadiUse™ PCR Reaction Buffer

ReadiUse™ PCR Reaction Buffer

Supplier: AAT BIOQUEST

This ready to use PCR buffer contains several components essential for the PCR reaction.

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Pierce TMT™ Elution Buffer

Pierce TMT™ Elution Buffer

Supplier: Thermo Fisher Scientific

Thermo Scientific TMT Elution Buffer is for use with Thermo Scientific Immobilized Anti-TMT Resin to enrich samples for peptides that have been chemically labeled with Tandem Mass Tag™ (TMT™) Reagents.

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Kinase buffer I (5X)

Supplier: Thermo Fisher Scientific

Kinase buffer I (5X)

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Phosphate-citrate Buffer (5X)

Supplier: Thermo Fisher Scientific

Phosphate-Citrate Buffer (5X) is used as a substrate buffer in immunoassay procedures. It is used in SDS PAGE electrophoresis.

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Kinase buffer II (5X)

Supplier: Thermo Fisher Scientific

Kinase buffer II (5X)

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Solubilisation buffer, 2D-Xtract™

Solubilisation buffer, 2D-Xtract™

Supplier: G-Biosciences

A protein solubilisation buffer for 2D analysis must solubilise proteins effectively, without disturbing the native charge of the proteins. Urea based solubilisation buffers solubilise proteins effectively, however can modify the native charge of the proteins, due to carbamylation. Urea exists in equilibrium with ammonium cyanate that modifies α- and ε-amino groups, inducing changes in the isoelectric point of proteins leading to artifactual results.

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