Human recombinant IL2 (fromE. coli)

PRSI91-011

Human recombinant IL2 (fromE. coli)

PRSI91-011

Pk:
50 µG
Protein/peptide type:
Recombinant
Source:
E. coli
Species:
Human
Environmentally Preferable:
Storage conditions:
Lyophilized protein should be stored at −20 °C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7 °C for 2-7 days. Aliquots of reconstituted samples are stable at −20 °C for 3 months.
Biological activity:
ED50 is less than 0.1 ng/ml as determined by the dose-dependent stimulation of murine CTLL-2 cells.
Specific Activity of 1.0 x 10⁷ IU/ mg.
Protein synonyms:
Interleukin-2|Aldesleukin|TCGF|IL-2|IL2|T-Cell Growth Factor
Protein/peptide name:
IL2
Purity:
> 95% as determined by reducing SDS-PAGE
Molecular weight:
15.4 kD
Sequence:
Pro22-Thr153
Formulation:
Lyophilized from a 0.2 um filtered solution of 20mM HAc-NaAc, 150mM NaCl, pH 4.0. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the Lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Tested applications:
Bioassay

Recombinant Human Interleukin-2 is a highly purified protein with a molecular weight of approximately 15,300 Daltons. The chemical name is des-alanyl-1, serine-125 Human Interleukin-2. It is produced by recombinant DNA technology using a genetically engineered E. coli strain containing an analog of the human interleukin-2 gene. Genetic engineering techniques were used to modify the Human IL-2 gene, and the resulting expression clone encodes a modified Human IL-2. This recombinant form differs from native Interleukin-2 in following ways: 1) it is not glycosylated; 2) the molecule has no N-terminal alanine; 3) the molecule has serine substituted for cysteine at amino acid position 125; 4) the aggregation state of molecule is likely to be different from that of native IL-2.