20196 Results for: "tert-Butyl+propargyl+ether&amp"

Supplier: Merck

These columns have been designed following the high demand for suitable analytical methods for process monitoring and quality control of wide pore biomolecules. Accurate analysis of proteins, antibodies and large peptides requires columns with good permeability, along with better mass transfer and selectivity. In order for size exclusion not to influence the separation, the pore size should be approximately ten times larger than the molecule being analysed.

Supplier: MP Biomedicals

Bromelain is a glycoprotein which is a highly active thiol proteinase (non-specific cysteine protease), which splits glycine esters but not lysine esters; it is also a protein-digesting and milk-clotting enzyme. Substrates susceptible to bromelain include all of the common protein materials such as gelatin, casein, gluten, collagen, elastin, globulins an muscle fiber protein. It is a cysteine endopeptidase with broad specificity for cleavage of proteins. It may be from a stem or piece of fruit.

Supplier: Cytiva

SP Sepharose™ High Performance is composed of crosslinked agarose beads with a mean diameter of 34 µm, modified with sulphopropyl (SP) strong cation exchange groups.

Supplier: USP

USP Reference Standards are specified for use in conducting official USP–NF tests and assays. USP also provides Reference Standards specified in the Food Chemicals Codex as well as authentic substances—high-quality chemical samples—as a service to analytical, clinical, pharmaceutical and research laboratories. To confirm accuracy and reproducibility, USP Reference Standards are rigorously tested and evaluated by multiple independent laboratories including USP, commercial, regulatory, and academic labs. USP also provide publicly available, official documentary standards for pharmaceutical ingredients in the USP–NF that link directly with our primary reference standards.

Supplier: Merck

Ascentis®Phenyl HPLC columns are optimised to the three terms of the resolution equation: efficiency, retention, and selectivity.

Supplier: MP Biomedicals

Quinine Hydrochloride is an alkaloid originally isolated from the Cinchona family of South American trees. It is an antimalarial agent, anticholinergic, antihypertensive and hypoglycemic agent.

Supplier: VELP SCIENTIFIC

The SER 148/3 and SER 148/6 can be used to separate a substance or a group of elements (e.g. fat) from solid and semi-solid samples according to the Randall technique (consisting of immersion, washing and solvent recovery). The main field of application is the determination of the content of soluble products such as fats, detergents, plasticisers and pesticides in food, animal feed, detergents, rubber and plastic formulas, pharmaceutical products, soil, and more.

Supplier: MP Biomedicals

Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.

Supplier: SIGMA ALDRICH MICROSCOPY

Araldite® is an epoxy resin commonly used for embedding. It is basically free from polymerization damage and the plastic does not sublime in the microscope, hence providing continual support and preservation of fine detail.[1] It is composed of araldite M or CY 212, dodecenyl succinic anhydride, dibutyl phthalate and 2,4,6-tridimethylamine methyl phenol.

Supplier: Sartorius

Elimination of particles from samples prior to HPLC or other chromatographic analysis is essential in order to maintain the integrity of the chromatography column and to maximise its operating life time. Minisart® syringe filters optimised for sample preparation consist of a polypropylene (PP) housing and membrane components featuring maximum chemical compatibility and minimum extractables to ensure excellent results. Due to the typical range of volumes from less than 1 ml to 100 ml these filters are available in three different diameters with an effective filtration area of 0,12, 1,7 and 4,8 cm².

Supplier: MP Biomedicals

Amphotericin B is a polyene antifungal antibiotic from Streptomyces. It has an affinity for sterols, primarily ergosterols, of fungal cell membranes. It forms channels in the membranes, causing small molecules to leak out which contributes to cell death.

Supplier: MACHEREY-NAGEL

QUANTOFIX® test strips meet all requirements of a modern rapid test. The colour of the reactive pad changes depending on the concentration of an analyte in the sample. The evaluation is usually carried out visually by comparison of the reaction colour with a multi-stage colour scale. These test strips are immediately ready-to-use. They do not require additional accessories. The test strips are intended for single use, maintenance or calibration are not required.

Supplier: Cytiva

ANX Sepharose™ 4 Fast Flow (high sub) is a weak anion exchanger, with a different selectivity compared with traditional ion exchangers.

Supplier: MP Biomedicals

Dissolves readily at 5 mg/ml in 0,01 M Sodium Phosphate pH 7,5 to give a clear colourless solution.

Supplier: MACHEREY-NAGEL

These crimp neck vials with different capacities are suitable for a large number of autosamplers included headspace GC applications.

Supplier: MP Biomedicals

Glutamate is a key compound in cellular metabolism. It is the most abundant excitatory neurotransmitter in the vertebrate nervous system. It also serves as the precursor for the synthesis of the inhibitory GABA in GABA-ergic neurons. This reaction is catalyzed by glutamate decarboxylase (GAD), which is most abundant in the cerebellum and pancreas.

Supplier: Thermo Fisher Scientific

Thermo Scientific Pierce EMCH is a mid-length, maleimide-and-hydrazide crosslinker for conjugating sulfhydryls (cysteines) to carbonyls (aldehyde or ketones, such as those formed by oxidation of glycoprotein carbohydrates).

Supplier: Thermo Fisher Scientific

Contains a C4 linker which allows for superior alignment of the phenyl ring with aromatic molecules. Get excellent retention and unique selectivity for aromatic and moderately polar analytes with Thermo Scientific™ Hypersil GOLD™ Phenyl reversed phase HPLC columns.

Supplier: MP Biomedicals

DL-Dithiothreitol is also known as Clelands reagent; Protective agent for sulfhydryl groups (-SH). Quantitatively reduces disulfides (-S-S- to -SH). In this reaction the DTT is oxidized to the cyclic disulfide which ensures the reduction of other disulfides in solution. Disulfide reduction occurs quickly at pH 8.
Dithiothreitol is useful for stabilizing sulfhydryl containing enzymes. Effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N'-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol.
Useful for stabilizing sulfhydryl-containing enzymes.

Supplier: Cytiva

HiScreen Phenyl HP columns are prepacked with Phenyl Sepharose High Performance hydrophobic interaction chromatography (HIC) resins. The columns are an excellent choice for method optimization and parameter screening.

Supplier: ENZO LIFE SCIENCES

Lipid peroxidation is a well-established mechanism of cellular injury in both plants and animals, and is used as an indicator of oxidative stress in cells and tissues. Lipid peroxides, derived from polyunsaturated fatty acids, are unstable and decompose to form a complex series of compounds. These include reactive aldehydes, of which the most abundant is malondialdehyde (MDA). Therefore, measurement of malondialdehyde is widely used as an indicator of lipid peroxidation. Increased levels of lipid peroxidation products have been associated with a variety of chronic diseases in both humans and model systems.

Supplier: TOSOH BIOSCIENCE

SkillPak 50 and 200 ml columns are designed for fast method development and scale-up and are pre-packed with TOYOPEARL® and TSKgel® process chromatography media. These columns offer superior separation of biomelocules, such as monoclonal antibodies, proteins and oligonucleotides. They are the best solution to scale up methods developed on SkillPak 1 and 5 ml pre-packed columns.

Supplier: Corning

Transwell® cell culture inserts are convenient, easy to use permeable support devices for the study of both anchorage-dependent and anchorage-independent cell lines. They are designed to produce a cell culture environment that closely resembles the in vivo state.

Supplier: ANTIBODIES.COM

Human MCPH1 ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human MCPH1 in serum, plasma, and other biological fluids.

Supplier: Thermo Fisher Scientific

Pierce™ Boronic acid resin enables ribonucleotide and oligonucleotide RNA isolation.

Supplier: OMEGA BIO-TEK

Isolate DNA from plant or fungal samples using spin columns.

Supplier: MP Biomedicals

Storage: Room Temperature, desiccate, store under nitrogen.
Dimethyl Sulfoxide is a dipola, aprotic solvent. It has been shown to accelerate strand renaturation (1-10% concentration) and is believed to give the nucleic acid thermal stability against depurination.
Dimethyl Sulfoxide is used to enhance dermal absorption of many chemicals, as a solvent for many organic and inorganic compounds including fats, carbohydrates, dyes, resins, and polymers, in antifreeze or hydraulic fluids, as a cryopreservative for cell cultures, oxidation of thiols and disulfides to sulfonic acids, as a PCR cosolvent to help improve yields, especially in long PCR. DMSO is routinely used in polymerase chan reaction (PCR), amplification of cDNA libraries, DNA sequencing, column-loading buffers for poly (A)+ RNA selection, buffers for the transformation of competent E. coli, and transfection protocols.
To prepare sterile solutions use a teflon or nylon membrane to sterile-filter the DMSO - do not use a cellulose acetate membrane.

Supplier: MP Biomedicals

Trypan Blue is a blue acid dye with a strong affinity for cellulose containing substrates such as cotton; less affinity for proteinaceous materials. Trypan blue solution may be used in trypan blue based cytotoxicity and proliferation assays.
Trypan Blue is used as a vital dye which is especially important because it is taken up by the reticuloendothelial system.

Supplier: MP Biomedicals

2,3,5-Triphenyltetrazolium Chloride is a redox indicator. In the TTC assay (also known as TTC test or tetrazolium test), TTC is used to differentiate between metabolically active and inactive tissues. The white compound is enzymatically reduced to red TPF (1,3,5-triphenylformazan) in living tissues due to the activity of various dehydrogenases (enzymes important in oxidation of organic compounds and thus cellular metabolism), while it remains as white TTC in areas of necrosis since these enzymes have been either denatured or degraded.
2,3,5-Triphenyltetrazolium Chloride is commonly used in biochemical experiments especially to indicate cellular respiration. TTC has been employed in autopsy pathology to assist identification of post-mortem myocardial infarctions. Healthy viable heart muscle will stain deep red from the cardiac lactate dehydrogenase; while areas of potential infarctions will be more pale. Useful indicator for reducing substances

Supplier: MP Biomedicals

Storage: Store at Room Temperature (15-30 °C)
Glycine is a non-essential amino acid. It is only amino acid with no asymmetric carbon and thus is not chiral. It is the major inhibitory neurotransmitter. It is involved in the biosynthesis of the porphyrin rings of hemes and chlorophylls.
Glycine is commonly used in buffer solutions, in electrophoresis and preparative chromatography. A study of the folding of monoclonal antibodies in the presence of glycine and their subsequent purification has been published. The use of glycine in the purification of lipopolysaccharides, lipooligosaccharides, and lipid A has been reported. It is an amino acid for use in cell culture media development applications and existing media formulations. Glycine is commonly used as a component in Tris-glycine and Tris-glycine-SDS running buffers for polyacrylamide gel electrophoresis, a component of Towbin's transfer buffer for Western blots, a buffer substance in cryoenzymology, in osmotic pressure maintenance in isoelectric focusing of erythrocytes, salting-in effect in protein chemistry, and as a buffer component in the coupled phosphatase-kinase reaction for end labelling of restriction fragments. The growth requirements of various microorganisms is reported in the Handbook of Microbiology.
Glycine is a non-chiral amino acid that can be synthesized in the body from the amino acid serine by Serine Hydroxymethyltransferase. Inhibitory neurotransmitter in spinal cord, allosteric regulator of NMDA receptors.