621 Results for: "chromatography preparative"

Supplier: MP Biomedicals

MP EcoChrom™ Alumina B, Activity Super I available as basic alumina is commonly used for purification processes of all kinds, including sample preparation for pesticides, and environmental analysis, solvent purification and all kinds of chromatography, requiring the highest precision and reproducibility.

Supplier: MP Biomedicals

Sodium phosphate dibasic heptahydrate is a reagent with very high buffering capacity widely used in molecular biology, biochemistry and chromatography. Sodium phosphate dibasic is highly hygroscopic and water soluble. Useful in conjunction with Sodium Phosphate, Monobasic in the preparation of biological buffers.

Supplier: Cytiva (Formerly Pall Lab)

The AcroPrep™ Advance filter plate portfolio includes a variety of membrane types and pore sizes to provide the best possible performance for a wide range of sample preparation and high throughput screening applications. AcroPrep™ Advance filter plates are ideal for concentration, purification and desalting of proteins and peptides; bead and chromatography resin-based applications; gross fractionation and lysate clarification, and general filtration.

Supplier: Cytiva (Formerly Pall Lab)

The AcroPrep™ Advance filter plate portfolio includes a variety of membrane types and pore sizes to provide the best possible performance for a wide range of sample preparation and high throughput screening applications. AcroPrep™ Advance filter plates are ideal for concentration, purification and desalting of proteins and peptides; bead and chromatography resin-based applications; gross fractionation and lysate clarification; and general filtration.

Supplier: Cytiva (Formerly Pall Lab)

The AcroPrep™ Advance filter plate portfolio includes a variety of membrane types and pore sizes to provide the best possible performance for a wide range of sample preparation and high throughput screening applications. AcroPrep™ Advance filter plates are ideal for concentration, purification and desalting of proteins and peptides; bead and chromatography resin-based applications; gross fractionation and lysate clarification and general filtration.

Supplier: Thermo Fisher Scientific

HyperSep™ positive pressure manifold can hold 48 SPE cartridges of various sizes 1, 3, 6, and 10/15 ml. Samples and solvent added to the SPE cartridges are extracted under positive pressure for an even flow at each position. Robust way to prepare multiple samples for subsequent analysis by chromatography.

Supplier: Cytiva (Formerly Pall Lab)

The AcroPrep™ Advance filter plate portfolio includes a variety of membrane types and pore sizes to provide the best possible performance for a wide range of sample preparation and high throughput screening applications. AcroPrep™ Advance filter plates are ideal for concentration, purification and desalting of proteins and peptides; bead and chromatography resin-based applications, gross fractionation and lysate clarification and general filtration.

Supplier: Cytiva (Formerly Pall Lab)

The AcroPrep™ Advance filter plate portfolio includes a variety of membrane types and pore sizes to provide the best possible performance for a wide range of sample preparation and high throughput screening applications. AcroPrep™ Advance filter plates are ideal for concentration, purification and desalting of proteins and peptides; bead and chromatography resin-based applications; gross fractionation and lysate clarification; and general filtration.

Supplier: Cytiva (Formerly Pall Lab)

The AcroPrep™ Advance filter plate portfolio includes a variety of membrane types and pore sizes to provide the best possible performance for a wide range of sample preparation and high throughput screening applications. AcroPrep™ Advance filter plates are ideal for concentration, purification and desalting of proteins and peptides, bead and chromatography resin-based applications, gross fractionation and lysate clarification, and general filtration.

Supplier: G-Biosciences

Ion exchange chromatography is used to separate charged molecules from complex biological samples. The charged molecules bind to a solid support carrying an opposite charge to the molecule. Proteins contain regions of charged groups on the surface which interact with the ion exchange groups immobilised on the solid support (resin column). Immobilised proteins are eluted by changing either pH or the salt gradient or a combination of both. This lab activity involves preparation of a crude protein extract and running ion exchange chromatography for isolation of proteins.

Supplier: Aviva Systems Biology

This product was prepared from normal serum by delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipi 1 * 1 mg

Supplier: Cytiva

MAbTrap™ is an affinity chromatography kit for fast and effective purification of monoclonal and polyclonal IgG from ascites fluid, serum and cell culture supernatants. MAbTrap™ Kit contains everything necessary to perform a complete one step purification without using complicated equipment. It also eliminates time consuming buffer preparation and column packing.

Supplier: MP Biomedicals

Elastase is prepared from porcine pancreas. It is lyophilised and water soluble. Elastase is chromatographically prepared by the method of Narayanan and Anwar. In the method, 2 times crystalline elastase is adsorbed on a column of DEAE-Sephadex A50 to separate elastase and non-specific protein components. The elastase component is further purified by chromatography on a column of CM-Cellulose using a sodium chloride gradient to elute the elastase. The latter is dialysed until chloride-free and then lyophilised. During its preparation the elastase is held below a pH of 5,5 for greater than 24 hours. Two times crystallised from the euglobin fraction of porcine pancreas by the method of Lewis et al. Does not contain trypsin or chymotrypsin.

Supplier: Thermo Fisher Scientific

Surfact-Amps™ detergents are ready-to-use, diluted solutions of purified detergent that can be used in routine and high-demand protein research methods and molecular biology techniques. These detergents are useful as a component of cell lysis buffers or other solutions intended to extract and solubilise proteins, improve chromatography, prepare yeast spheroplasts, and reduce background in ELISA or Western blot methods.

Supplier: Whatman products (Cytiva)

Whatman Puradisc micron syringe filters from Cytiva's offer a versatile solution for analytical sample preparation needs. They are a variety of options available to suit all labs needs, and are well-suited for routine syringe filtration of samples up to 100 mL for a range of applications, including high performance liquid chromatography (HPLC) and capillary electrophoresis (CE).

Supplier: SIGMA ALDRICH MICROSCOPY

Naphthol blue black (NBB) is an textile diazo dye, with high photo- and thermal-stability. Photoelectrochemical degradation of this dye yields colorless quinone as end product. Pure grade of NBB is generally used for dyeing foodstuffs, drugs and cosmetics. It is also used as a protein stain in chromatography and electrophoresis and as a biological stain. Commercial grade of NBB is a widely used textile dye. It is used in dyeing wool, nylon, silk and in textile printing. In addition, it is also employed for colouring of soaps, anodised aluminum and casein, wood stains and writing ink preparation.

Supplier: G-Biosciences

ECH-Agarose contains carboxyl functional group for coupling of affinity ligands via their amine groups for affinity chromatography. ECH-Agarose is prepared by condensation of Carboxyl Coupling Resin (Cat. # 786-797) with succinic anhydride, resulting 1 * 50 mL

Supplier: Sartorius

Elimination of particles from samples prior to HPLC or other chromatographic analysis is essential in order to maintain the integrity of the chromatography column and to maximise its operating life time. Minisart® syringe filters optimised for sample preparation consist of a polypropylene (PP) housing and membrane components featuring maximum chemical compatibility and minimum extractables to ensure excellent results. Due to the typical range of volumes from less than 1 ml to 100 ml these filters are available in three different diameters with an effective filtration area of 0,12, 1,7 and 4,8 cm².

Supplier: G-Biosciences

ECH-Agarose contains carboxyl functional group for coupling of affinity ligands via their amine groups for affinity chromatography. ECH-Agarose is prepared by condensation of Carboxyl Coupling Resin (Cat. # 786-797) with succinic anhydride, resulting 1 * 15 mL

Supplier: Biotium

CF™488A goat anti-human IgM (μ chain) is prepared from antibodies purified by human IgM-affinity chromatography. The antibody reacts specifically with IgM heavy chains (μ chains) and not with immunoglobulin light chains. To minimize crossreactivity, it has been adsorbed against human IgG prior to conjugation. Green fluorescent CF™488A (Ex/Em 490/515 nm) is a superior alternative to FITC (or fluorescein) owing to its exceptional brightness and photostability.

Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.

Supplier: Merck

The tricyclazole CRM may also find the following uses: Determination of tricyclazole content in paddy rice using sliver colloid as a substrate and pyridine as an internal standard by surface-enhanced Raman spectroscopy (SERS). To detect residues of tricyclazole and thiram in pear fruit extracts using SERS. Dissipation studies of penoxsulam tricyclazole and profoxydim in rice paddy systems using HPLC-Diode Array Detection (DAD). Determination of tricyclazole residues in rice using liquid chromatography-tandem mass spectrometry (LC-MS/MS) along with the QuEChERS method of sample preparation. Degradation of tricyclazole (TC) in an aqueous solution and real pesticide industry wastewater (RPW) by electron beam (EB) radiation.

Supplier: Merck

Synergy® ultrapure water systems are ideal for laboratories with access to purified water that needs a 'point of use' supply of high quality ultrapure water. The Synergy® system is suitable for users needing 2 to 20 L/day. The system produces Type I water from pre-treated water at a flow from 1 to 1,5 L/min. The water produced is suitable for the preparation of buffers for liquid chromatography, biochemical experiments, isocratic or gradient solutions for HPLC analysis, culture media for bacteriological analysis or cell cultures and solutions for spectrophotometry, spectroscopy and other analytical techniques.

Supplier: Thermo Fisher Scientific

Poly(vinylidene fluoride) is useful in coatings, film, filter cloth, instrument linings, filtration membranes, pump parts, linings and valves. It is also employed as a material for transducers in devices such as headphones, microphones and sonic detectors. It is also essential for piezoelectric and electrostrictive applications. In addition, it is useful for monofilament fishing lines replacing nylon monofilament. It acts as a standard binder used in the production of composite electrodes for lithium ion batteries. It serves as a high grade insulator for wires, nanomaterials and pressure sensors. It is utilized to make filters which are used to prepare samples for high performance liquid chromatography and other advanced analytical techniques.
Notes: Incompatible with strong oxidizing agents.

Supplier: MP Biomedicals

Goat IgG fraction to beta-Galactosidase is used in enzyme Immunoassays, fluorescein Immunoassays, blots, cell or tissue immunostaining. Gives sharp precipitation lines in IEP and ID techniques.
The antibody is produced by hyperimmunizing goats with the enzyme beta-galactosidase from Escherichia coli. The IgG fraction is prepared by delipidation, ammonium sulfate fractionation, and anion exchange chromatography, and is further purified by solid-phase absorption to remove antibodies to non-beta-galactosidase E. coli proteins. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 μm filter, and vialed. No preservative is added.

Supplier: GRANT INSTRUMENTS

A range of eco-friendly, refrigerated/heating circulating baths with an intuitive user-interface and sleek design. Units offer significant running cost savings whilst delivering powerful cooling when needed. Choice of 2 models with temperature range of –25 to +150 °C (model dependent*). Ideal for applications in pharmaceutical, education, industrial and life science areas for use with mini pilot plant reactors, cooling rotary evaporators, replacing running tap water cooling, immersing small samples, photometry, chromatography systems, sample preparation, general cooling, reaction chemistry, temperature control, semi-conductor manufacturing, rheometry, refractometry and electrophoresis cooling.

Supplier: Thermo Fisher Scientific

Halt Protease Inhibitor Cocktail provides all the protection of a broad-spectrum protease inhibitor in a single, ready-to-use liquid format. Halt protects against protein degradation by serine, cysteine, and aspartic acid proteases and aminopeptidases. Metalloproteases are inhibited by the optional use of 0,5 M EDTA. The 100X Halt stock solution makes it easy to dispense the required amount (10 µl per 1 ml) of protease inhibitors into buffers or lysis reagents prior to extraction of proteins from cultured cells, animal tissues, plant tissues, yeast or bacteria. Halt Protease Inhibitor Cocktails are compatible with Thermo Scientific Pierce Protein Extraction Reagents. Halt EDTA-free formulation is ideal for preparing samples that will be analysed by 2-D gel electrophoresis, immobilised metal chelate affinity chromatography (IMAC) and other downstream application where chelating agents interfere.

Supplier: MP Biomedicals

The IgG fraction is prepared from the specific rabbit antiserum by delipidation, ammonium sulfate fractionation and anion exchange chromatography. The resulting IgG fraction is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized. No preservative is added.

The total protein is measured using the Biuret procedure, with bovine albumin as a standard. Hemagglutination titer is standardized with an in-house control by measurement of agglutination end-point with 10% human red blood cells. Each IgG fraction is tested for purity at a minimum of 40 mg/ml using immunoelectrophoresis. The product is mostly rabbit IgG; no trace of albumin is detected.

Supplier: MP Biomedicals

This product is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction to rabbit IgG (whole molecule) and buffer salts. Anti-Human IgG is developed in goat using IgG from pooled normal human serum as the immunogen. Whole antiserum is fractionated and then further purified by ion exchange chromatography to provide the IgG fraction of antiserum. This fraction is essentially free of other goat serum proteins. Specificity for human IgG is determined by immunoelectrophoresis (IEP) versus purified human IgA, IgG, IgM, Bence Jones kappa, and Bence Jones lambda myeloma proteins.Identity and purity of the antibody is established by immunoelectrophoresis (IEP), prior to conjugation. Electrophoresis of the antibody preparation followed by diffusion versus anti-goat IgG and anti-goat whole serum result in single arcs of precipitation in the gamma region.

Supplier: Merck

Direct-Q® 3 systems have been developed for simple, immediate use. Ultrapure Type I water is suitable for preparing buffers for liquid chromatography and biochemical experiments, and for analytical procedures such as spectrophotometry and spectroscopy. For applications that require very low values of organic impurities, the Direct-Q® 3 UV system includes a UV lamp with two wavelengths in order to produce water with a TOC content <5 ppb, which is suitable for HPLC, GC, ILC and TOC analyses. With the BioPak™ cartridge as a final filter, Direct-Q® 3 systems produce pyrogen- and RNase-free water at a flow rate of 0,2 L/min. Direct-Q® 3 systems are ideal for laboratories which do not have easy access to pre-treated water and require between 5 and 10 litres of ultrapure water per day. For the production of Type I and Type III water.

Supplier: MP Biomedicals

Product is rabbit IgG (whole molecule) covalently coupled to Sepharose-4B, and packed in a polystyrene chromatography column.IgG affinity gel contains a proprietary ligand that retains most protein found in serum, ascites and culture supernatants, while allowing IgG to pass through the support and be collected in the flow-through fraction. Because this gel is not a bind-and-release support, it is extremely fast and gentle to antibodies, resulting in antibody preparations of high purity and high activity. IgG affinity gel works with antibodies from a variety of species and subclasses, many of which do not purify efficiently with Protein A or Protein G. The resulting recovery and purity of the IgG isolated by this method rivals that obtained from the same samples using bind-and-release supports such as Protein A or Protein G. Furthermore, antibodies purified with this gel are recovered ready for use in immunoassays or subsequent conjugation or modification steps; there is no need to neutralize or desalt the recovered antibody to remove harsh elution buffers.