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621 results for "chromatography preparative"

 

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Millex™ HV Syringe Filter

Millex™ HV Syringe Filter

Supplier: Merck

Millex-HV Syringe Filter with PVDF Membrane bind far less protein than nylon, nitrocellulose membranes, or PTFE membranes. High flow rates and throughput, low levels of extractables and broad chemical compatibility.

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Immobilised polysaccharide columns

Immobilised polysaccharide columns

Supplier: CHIRAL TECHNOLOGIES

These columns allow chromatographers to employ essentially any organic solvent as mobile phase to develop methods for the most challenging separations. These columns are available in a variety of particle sizes and offer great speed and column resolving power. Columns packed with 3 µm CSPs offer high speed separations and a reduction in analysis time and cost per sample. Columns, packed with 3 µm particles, may be used in conventional HPLC units without significant loss in performance, even with the smaller column dimensions.

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His MultiTrap™ FF and HP filter plates prepacked with Sepharose™

His MultiTrap™ FF and HP filter plates prepacked with Sepharose™

Supplier: Cytiva

His MultiTrap™ FF and His MultiTrap™ HP are prepacked disposable 96-well plates for reproducible high throughput parallel purification of histidine-tagged recombinant proteins by Immobilised Metal ion Affinity Chromatography (IMAC).

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HPLC columns, ZORBAX® 80 Å StableBond®

HPLC columns, ZORBAX® 80 Å StableBond®

Supplier: Agilent

ZORBAX® 80 Å StableBond columns are made using bulky, patented, unique silanes that sterically protect the siloxane bond. Acid labile endcapping reagents are not used.

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Pierce™ BCA™ Protein assay kits and reagents

Pierce™ BCA™ Protein assay kits and reagents

Supplier: Thermo Fisher Scientific

Pierce BCA™ (bicinchoninic acid) reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA™ Protein Assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA™ Assay is affected much less by protein compositional differences, providing greater protein-to-protein uniformity.

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Multimode columns, HiTrap™ Capto™ MMC

Multimode columns, HiTrap™ Capto™ MMC

Supplier: Cytiva

HiTrap Capto MMC is prepacked with Capto MMC, a multimodal BioProcess ion exchange resin. The multimodal functionality gives a different selectivity compared with traditional ion exchangers and it binds proteins at high or low ionic strength.

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Albumin and IgG depletion SpinTrap™ columns

Albumin and IgG depletion SpinTrap™ columns

Supplier: Cytiva

Albumin and IgG depletion SpinTrap™ columns for the depletion of albumin and IgG from human serum and plasma. Columns are prepacked with high performance Sepharose-based media with an affinity for human serum albumin (HSA) and IgG.

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Ammonium carbonate, white powder

Supplier: MP Biomedicals

Ammonium carbonate is used in the washing and defatting of wools, the manufacture of rubber products, in fire extinguishers, and the separation of cacao constituents.

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Protease inhibitor cocktail, Bacterial ProteaseArrest™

Protease inhibitor cocktail, Bacterial ProteaseArrest™

Supplier: G-Biosciences

A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail, Bacterial ProteaseArrest™ inhibits bacterial serine, cysteine and other bacterial specific proteases, including aminopeptidases and aspartic proteases.

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Disposable PD-10 desalting columns

Disposable PD-10 desalting columns

Supplier: Cytiva

PD-10 Desalting Columns contain Sephadex G-25 resin for rapid buffer exchange, desalting, and removal of small contaminants (salts, dyes, radioactive labels) from samples using gravity flow or centrifugation.

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Columns for large biomolecule analysis, CIMmultus™

Supplier: HICHROM

CIMmultus™ columns are based on an innovative monolithic technology. Distinguished by large pores/flow through channels these columns are designed for the production, purification, and analytics of large proteins (IgG, IgM, PEGylated proteins), viruses (AAV, adenovirus, lentivirus, influenza, bacteriophages), VLPs, exosomes and pDNA for example.

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N,O-Bis(trimethylsilyl)trifluoroacetamide with 1% trimethylchlorosilane Silyl-991 silylation reagent, for GC derivatisation

N,O-Bis(trimethylsilyl)trifluoroacetamide with 1% trimethylchlorosilane Silyl-991 silylation reagent, for GC derivatisation

Supplier: MACHEREY-NAGEL

Silyl-991 is recommended for silylation of fatty acid amides, hindered hydroxyls and other compounds which are difficult to silylate (e.g., secondary alcoholes and amines).

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BioHPLC columns, DNAPac™ RP

BioHPLC columns, DNAPac™ RP

Supplier: Thermo Fisher Scientific

Achieve superior reversed phase (RP) oligonucleotide separatons using the DNAPac™ RP HPLC columns. The unique chemistry is designed for analysis of oligonucleotides and double-sranded (DS) DNA/RNA fragments using LC-UV or LC-MS. The column chemistry provides excellent performance under a broaed range of pH, temperature and mobile phase composits.

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N,O-Bis(trimethylsilyl)acetamide: 1-(trimethylsilyl)imidazole: hexamethyldisilazane (BSA+TMSI+HMDS) mixture Silyl-271 silylation reagent, for GC derivatisation

N,O-Bis(trimethylsilyl)acetamide: 1-(trimethylsilyl)imidazole: hexamethyldisilazane (BSA+TMSI+HMDS) mixture Silyl-271 silylation reagent, for GC derivatisation

Supplier: MACHEREY-NAGEL

Silyl-271 will derivatise all hydroxyl groups in any position. Useful in multiderivatisation schemes involving hydroxyl or amine groups.

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Anion exchange columns, CHIRALPAK® QD-AX and QN-AX

Supplier: CHIRAL TECHNOLOGIES

CHIRALPAK® QN-AX and CHIRALPAK® QD-AX are enantioselective weak anion-exchange (AX) HPLC columns. The chiral selectors for CHIRALPAK® QD-AX and CHIRALPAK® QN-AX anion exchange phases are based on complementary quinine (QN) and quinidine (QD) derivative selectors bonded to high efficiency 5 µm diameter spherical silica. The structure of these selectors is such that a tertiary amine is located in a three-dimensional cleft surrounded by additional hydrogen binding sites and a large aromatic group. This combination provides excellent separations of chiral acids.

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Affinity chromatography media, Lentil Lectin Sepharose™ 4B

Supplier: Cytiva

Lentil lectin is a metalloprotein, containing Ca₂₊ and Mn₂₊, which binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars.

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TSKgel® gel filtration columns, PW-type

Supplier: TOSOH BIOSCIENCE

These columns are designed for GFC of water soluble organic polymers, polysaccharides, oligosaccharides, DNA and RNA. They are based on a hydrophilic polymethacrylate matrix, which is stable from pH 2 to 12 and in aqueous eluents with up to 50% polar organic solvent.

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Hygromycin B solution 50 mg/ml, sterile

Supplier: Corning

Hygromycin B, produced by Streptomyces hygroscopicus, is used as a selection agent and inhibits protein synthesis in cells not carrying hygromycin phosphotransferase (HPH)

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Anti-IgG Goat Polyclonal Antibody

Supplier: AAT BIOQUEST

Anti-Human IgG (Fc specific) antiserum is produced in goat using purified human IgG, Fc fragment, as the immunogen.

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Protease inhibitor cocktail, Mammalian ProteaseArrest™

Protease inhibitor cocktail, Mammalian ProteaseArrest™

Supplier: G-Biosciences

A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail, Mammalian ProteaseArrest™ inhibits mammalian serine, cysteine and other mammalian specific proteases including aminopeptidases, trypsin-like and aspartic proteases.

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Human Recombinant PPM1B (NM_177968) Protein (C-Myc/DDK Tag)

Supplier: OriGene

Recombinant protein of human protein phosphatase 1B (formerly 2C), magnesium-dependent, beta isoform (PPM1B), transcript variant 2, 100 µg.

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Ni-NTA spin columns, HisPur™

Ni-NTA spin columns, HisPur™

Supplier: Thermo Fisher Scientific

Pierce™ Ni-NTA spin columns contain a high-capacity, high-performance nickel-IMAC resin for routine affinity purification of His-tagged fusion proteins.

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Ion exchange chromatography media, SOURCE™ 15Q

Ion exchange chromatography media, SOURCE™ 15Q

Supplier: Cytiva

Mono-sized 15 um rigid beads gives low back pressure and high flow rates allowing for high productivity.

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MP-AES torches

MP-AES torches

Supplier: Agilent

MP-AES Torches for the 4100/4200 Microwave Plasma-Atomic Emission Spectrometer.

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Cleanroom gloves, Microflex® 93-360

Cleanroom gloves, Microflex® 93-360

Supplier: Ansell

Microflex® 93-360 is a thin, chemical resistant disposable glove designed for cleanroom environments. It is made of an innovate, synthetic composite material (nitrile/neoprene) for tough chemical protection.

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Gel filtration media, Sephadex™ G-15

Gel filtration media, Sephadex™ G-15

Supplier: Cytiva

Sephadex™ G-15 is a well established gel filtration medium for desalting and buffer exchange of peptides and small biomolecules >1500 molecular weight.

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Affinity purification resins, UltraLink™ iodoacetyl resin

Affinity purification resins, UltraLink™ iodoacetyl resin

Supplier: Thermo Fisher Scientific

UltraLink™ iodoacetyl resin is a durable, porous resin that is activated to enable efficient and direct covalent immoblisation of peptides and other ligands through their sulfhydryl groups (-SH) for use in affinity purification procedures.

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Savant™ SpeedVac™ SPD130P1 Medium Capacity Concentrator for Combinatorial Chemistry Applications

Savant™ SpeedVac™ SPD130P1 Medium Capacity Concentrator for Combinatorial Chemistry Applications

Supplier: THERMO SAVANT

The Thermo Scientific™ Savant™ SpeedVac™ SPD130DLX vacuum concentrator is a medium-capacity, vacuum concentrator with a modular design. It is compatible with a wide variety of solvents and offers resistance to TFA, DMSO, and other aggressive solvents used in combinatorial chemistry applications.

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Gel filtration media, Sephadex™ G-100

Gel filtration media, Sephadex™ G-100

Supplier: Cytiva

Sephadex™ G-100 is useful for desalting and buffer exchange of very large biomolecules. Sephadex™ G-100 Superfine is designed for specific cases requiring very high resolution.

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Spin filter plates, 96 well, for Ni-NTA proteins, HisPur™

Spin filter plates, 96 well, for Ni-NTA proteins, HisPur™

Supplier: Thermo Fisher Scientific

The HisPur™ Ni-NTA spin plates contain a high-capacity, high-performance nickel-IMAC resin for routine affinity purification of His-tagged fusion proteins.

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