Supplier: EDVOTEK

In this experiment, students purify the restriction enzyme, Eco RI. This procedure utilised an ion exchange chromatograph step for Eco RI purification. Column fractions are assayed for the enzyme using Lambda DNA and digestion products are identified by gel electrophoresis. Fractions that contain Eco RI are identified and pooled. The total and specific activities are calculated. Recommended for advanced courses.

Supplier: AAT BIOQUEST

Horseradish peroxidase (HRP) and HRP conjugates facilitate the ABTS oxidation in the presence of hydrogen peroxide, turning ABTS into its blue-green oxidized product.

Supplier: MACHEREY-NAGEL

A pre-dosed NANOCOLOR® tube test for photometric determination of TOC (total organic carbon) in water.

Supplier: Thermo Fisher Scientific

Yeast β-Galactosidase Assay Kit is specially formulated for yeast cells and ideal for identifying protein interactions in vivo using two-hybrid systems.

Supplier: LABCON

Ultra-clear polypropylene, graduated, autoclavable, and freezable microcentrifuge tubes with flat cap. Amber coloured styles are available for use with light-sensitive substances.

Supplier: VWR Chemicals

Avantor now offers a complete range of COD (Chemical Oxygen Demand) calibration standards (traceable to BAM) as CRM with concentrations between 20 and 10000 mg/L. Can be used to calibrate, control, verify or validate all COD test methods.

Supplier: Thermo Fisher Scientific

This BCA Protein Assay Kit - Reducing Agent Compatible enables the measurement of protein concentration in samples that contain thiol-reductants dithiothreitol (DTT) and 2-mercaptoethanol (BME).

Supplier: Thermo Fisher Scientific

The Pierce™ Coomassie Plus Protein assay is a quick and ready-to-use, reducing agent-compatible, improved Bradford assay to quickly measure (A595 nm) total protein concentration compared to a protein standard.

Supplier: LABCON

Ultra-clear polypropylene, graduated, autoclavable, and freezable microcentrifuge tubes with flat caps. Amber coloured styles are available for use with light-sensitive substances.

Supplier: GRANT INSTRUMENTS

The DEN-600 is a compact, portable, rechargeable battery powered photometer for OD600, McF turbidity and Bradford protein assay methods.

Supplier: MOLECULAR DEVICES

This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction.

Supplier: WATER ID PRIMELAB

The next generation of multitest Photometer, with pH - EC - TDS - ORP - Temperature measurement functionality.

Supplier: G-Biosciences

Ion exchange chromatography is used to separate charged molecules from complex biological samples. The charged molecules bind to a solid support carrying an opposite charge to the molecule. Proteins contain regions of charged groups on the surface which interact with the ion exchange groups immobilised on the solid support (resin column). Immobilised proteins are eluted by changing either pH or the salt gradient or a combination of both. This lab activity involves preparation of a crude protein extract and running ion exchange chromatography for isolation of proteins.

Supplier: G-Biosciences

This kit teaches aseptic handling techniques and cultivation of bacteria in liquid culture media and on solid phase agar plates. This kit is designed to educate students about the various stages of the bacterial growth cycle, i.e. lag, log or exponential, stationary and decline or death phases. The kit also teaches the importance of growing bacteria on solid phase agar plates to isolate single colonies of bacteria. This lab activity involves preparation of culture medium and solid agar plates.

Supplier: EDVOTEK

Coronary heart disease and stroke are major causes of death in the Western world. Elevated blood cholesterol levels are a serious risk factor in both conditions. The genetic disease familial hypersholesterolemia (FH) causes an increase in blood levels of the bad form of cholesterol, low density lipoprotein (LDL). In untreated patients with the mutant FH gene, the condition can cause premature death. This experiment introduces the colorimetric enzymatic reaction which is the basis of the clinical cholesterol test. In addition, using agarose gel electrophoresis, students can analyse a simulated genetic screening for a disease.

Supplier: Thermo Fisher Scientific

Pierce™ Glycoprotein Carbohydrate Estimation Kit enables the amount of protein glycosylation to be measured as a percentage of total purified protein mass.

Supplier: Thermo Fisher Scientific

Pierce BCA™ (bicinchoninic acid) reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA™ Protein Assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA™ Assay is affected much less by protein compositional differences, providing greater protein-to-protein uniformity.

Supplier: EDVOTEK

When DNA is subcloned in the pUC polylinker region, β-galactosidase production is interrupted, resulting in the inability of cells to hydrolyse X-Gal. This results in the production of white colonies amongst a background of blue colonies. This experiment provides a DNA fragment together with a linear plasmid and T4 DNA ligase. Following the ligation to synthesis the recombinant plasmid, competent E. coli cells are transformed and the number of recombinant antibiotic-resistant white and blue colonies are counted. β-galactosidase activity is assayed from blue and white bacterial cells.

Supplier: G-Biosciences

NUCLEIC dotMETRIC™ Kit provides a quick and accurate method for detecting and measuring nucleic acid concentration, including DNA, RNA and oligonucleotides. The system uses a combination of proprietary reagents and test strips and is an alternative to spectrophotometry as no equipment or cuvettes are necessary. NUCLEIC dotMETRIC™ uses as little as one microlitre of sample and provides permanent results within 2 to 3 minutes.

Supplier: Thermo Fisher Scientific

The Thermo Scientific Pierce™ Chromogenic endotoxin Quant kit is a highly sensitive endpoint assay that accurately measures and detects endotoxin (lipopolysaccharide) in a protein, peptide, nucleic acid, or antibody sample using the amebocyte lysate assay. The kit enables detection within two linear sensitivity ranges of 0,01 to 0,1 EU/ml and 0,1 to 1,0 EU/ml.

Supplier: BIOCHROM

This software is supplied in different modules to meet the application requirements of different customer groups and to offer a simple upgrade path should your requirements change.

Supplier: Thermo Fisher Scientific

The Mammalian β-Galactosidase Assay Reagent Kit is a simple and versatile assay to measure β-galactosidase activity in cultured mammalian cells. Cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single all-in-one lysis-and-assay solution. Alternatively, M-PER™ Mammalian Protein Extraction Reagent may be used to prepare lysate from washed adherent or pelleted cells, and then a portion of the lysate used to assay for β-galactosidase activity. With M-PER Reagent, there is no need to scrape cells or to perform freeze-thaw cycles. The remaining lysates prepared with M-PER™ Reagent may be used for applications such as reporter assays (luciferase, β-galactosidase and chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, and tyrosine kinase), immunoassays (Western blot, ELISA, RIA) and protein purification.

Supplier: G-Biosciences

Biodiversity lab activity is designed for determination of protein contents and biomass in diverse biological samples to study how biomass is related to biodiversity in nature. Students collect and catalogue plant leaf samples from a diverse group of locally available plants. This lab activity involves determination of natural weight of each plant sample, grinding a predetermined amount of each sample, and the subsequent extraction of proteins from the samples. Students then learn to determine protein contents of each plant sample and attempt to relate the protein content with biomass. Students in this lab activity are challenged to think, analyse, and seek answers as to why protein biomasses vary for a given natural weight for different plants. Finally, they will relate that finding to the biodiversity of nature.

Supplier: ANVAJO

The fluidlab R-300 combines an automated cell counter with a full-fledged spectrometer, thus enabling a variety of analyses such as automated cell counting and staining-free viability measurements as well as scatter light, absorbance and time-series measurements. With its intuitive handling and reliable results, the portable device is the all-rounder for your everyday laboratory work.

Supplier: G-Biosciences

Determination of protein concentration is an essential technique in all aspects of protein studies and proteomics. The Assays for Protein Quantification kit includes three of the most widely used protein assays and allows for a direct comparison of the three assays that teaches students the benefits and limitations of each assay. Each assay is available individually to allow teaching of a specific assay, without the option of comparing and contrasting with other assays. The three assays covered are the Biuret Protein Assay, Lowry Protein Assay and the Coomassie Blue Dye Protein Assay. The Assays for Protein Quantification kit provides all the reagents required to perform both protein assays, including protein standards for accurate quantification, in a single lab activity. An often underestimated factor in quantifying protein is the presence of non-protein interfering agents, such as salts and detergents. This kit teaches students about common laboratory agents that affect the protein assays, the reasoning behind their interferences and how to overcome the interference. Students also learn how to select a protein assay for different applications.

Supplier: MP Biomedicals

The SPINeasy® DNA Pro Kit for Feces is able to handle a wide range of fecal samples.