"Spectrophotometer"

Pierce™ BCA™ Protein assay kits and reagents

Supplier: Thermo Fisher Scientific

Pierce BCA™ (bicinchoninic acid) reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA™ Protein Assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA™ Assay is affected much less by protein compositional differences, providing greater protein-to-protein uniformity.

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SPECTROPHOTOMETER RT 520 1 * 1 items

Supplier: Lovibond Tintometer

SPECTROPHOTOMETER RT 520 1 * 1 items

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SPECTROPHOTOMETER GENOVA BIO 1 * 1 items

Supplier: Cole-Parmer

SPECTROPHOTOMETER GENOVA BIO 1 * 1 items

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Experimentation kits, in search of the cholesterol gene

Supplier: EDVOTEK

Coronary heart disease and stroke are major causes of death in the Western world. Elevated blood cholesterol levels are a serious risk factor in both conditions. The genetic disease familial hypersholesterolemia (FH) causes an increase in blood levels of the bad form of cholesterol, low density lipoprotein (LDL). In untreated patients with the mutant FH gene, the condition can cause premature death. This experiment introduces the colorimetric enzymatic reaction which is the basis of the clinical cholesterol test. In addition, using agarose gel electrophoresis, students can analyse a simulated genetic screening for a disease.

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Experimentation kits, bacterial culture and growth study

Supplier: G-Biosciences

This kit teaches aseptic handling techniques and cultivation of bacteria in liquid culture media and on solid phase agar plates. This kit is designed to educate students about the various stages of the bacterial growth cycle, i.e. lag, log or exponential, stationary and decline or death phases. The kit also teaches the importance of growing bacteria on solid phase agar plates to isolate single colonies of bacteria. This lab activity involves preparation of culture medium and solid agar plates.

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Experimentation kits, ion exchange chromatography

Supplier: G-Biosciences

Ion exchange chromatography is used to separate charged molecules from complex biological samples. The charged molecules bind to a solid support carrying an opposite charge to the molecule. Proteins contain regions of charged groups on the surface which interact with the ion exchange groups immobilised on the solid support (resin column). Immobilised proteins are eluted by changing either pH or the salt gradient or a combination of both. This lab activity involves preparation of a crude protein extract and running ion exchange chromatography for isolation of proteins.