911 Results for: "SIGMA ALDRICH MICROSCOPY"

Supplier: SIGMA ALDRICH MICROSCOPY

Phosphate buffer's buffering capacity is pH sensitive and is preferred to be used at physiological pH (7,2 to 7,4).

Supplier: SIGMA ALDRICH MICROSCOPY

PolyFreeze is a water-soluble support matrix and a form of embedding medium for frozen sectioning. PolyFreeze sections flawlessly at routine frozen specimen thickness of 5-6 μ and leaves no slide residue. It provides for -20 ºC cryostat sectioning in rapid set embedment. This medium freezes quickly supporting the tissue for sectioning at 3 mm and up with no cracking of the matrix at temperatures from -8 °C to -25 °C.

Supplier: SIGMA ALDRICH MICROSCOPY

ImmunoHistoMount™ is a permanent aqueous mounting medium designed for tissue sections and cell smears with peroxidase and alkaline phosphatase chromogens that cannot be dehydrated with organic solvents.

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Cell permeable fluorescent detector of nitric oxide in living cells. 4,5-Diaminofluorescein diacetate (DAF-2 DA) has been used as a fluorescent dye for NO (nitric oxide) detection.

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Fluorescein isothiocyanate isomer I–Celite® has been conjugated with antibody for fluorescence imaging in cells and flow cytometry analysis.

Supplier: SIGMA ALDRICH MICROSCOPY

Erythrosin extra bluish is a brown color powder, that is soluble in ethanol and water. Its pH ranges from 2,5 to 4. It is used in color filters, light emitting diodes, cosmetics, hair dyes and more. It has several biological applications like detecting gene expression, treating age related macular degeneration, diabetes, obesity and more.

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Erythrosin B, also called as eosin B, is a xanthene dye. It belongs to the family of fluorescein dyes. It plays a major role in inhibiting dopamine uptake and high affinity 3H-ouabain binding and ion transport in synaptosomes from rat caudate nucleus. Erythrosin B has been used as a dye in fluorescence lifetime imaging.

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Bismarck brown Y, Sigma-Aldrich®

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Bromophenol blue (BroB), an arylmethane color marker dye has a molecular mass of 670 Da.

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Phenol Red Sodium Salt (PRSS) is a water-soluble anionic fluorescent dye, that possesses an extra Na ion attached with the SO3 group compared to phenol red. It is intended for use in cell culture applications. A solution of phenol red will have a yellow color at a pH of 6,4 or below and a red color at a pH of 8,2 and above.

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Tetrabromophenol Blue (TBPB) belongs to the group of sulfonephthalein acid-base indicators. It is employed in the potentiometric titration of the halogens with Hg₂(NO₃)₂. In addition, it has been used as a dye indicator in urine dipstick test to detect proteinuria. In this assay, with increasing concentration of urinary protein, TBPB sequentially changes colour from pale green to blue. Binding of TBPB to proteins is dependent on pH.

Supplier: SIGMA ALDRICH MICROSCOPY

Azobenzene 98%, Sigma-Aldrich®

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Disperse Blue 134, for microscopy (1,4-Bis(isopropylamino)anthraquinone; Solvent Blue 36).

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Silver enhancer solution A has been used for silver staining.

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Azophloxine has been used as a dye in Von Kossa staining and nonlinear optical studies. It is also used in photocatalytic tests.

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Fluorescent dye.

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Bromophenol Blue–Xylene Cyanol Dye solution has been used in the formamide loading buffer for the size purification of ligated RNA from polyacrylamide gel.

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Cardiogreen was injected in animals intravenously for dynamic optical imaging system to study the capability of capturing the in vivo biodistribution kinetics.

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Gallocyanine, Dye content 90 % (7-Dimethylamino-4-hydroxy-3-oxo-phenoxazine-1-carboxylic acid)

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For use in the detection of phospholipids and related compounds.

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Prepared by alkaline hydrolysis.

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Rhodamine B is a thiol-reactive and photostable fluorescent dye. Rhodamine B isothiocyanate is an aminoxanthene that possesses carboxylic acid and isothiocyanate substituents on the pendant phenyl ring.

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Fluorescein isothiocyanate (FITC) is yellow-orange in color with an absorption maximum at 495nm. Upon excitation, it emits a yellow-green color with an emission maximum at 525 nm. It is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins. Fluorescein isothiocyanate isomer I has been proposed as a contact sensitiser.

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Nitrotetrazolium blue (NBT), in combination with BCIP (5-bromo-4-chloro-3-indolyl-phosphate), is required for the detection of labeled probes in western blots. It is part of the colorimetric detection assay. NBT and BCIP dyes detect alkaline phosphatase activity, thereby generating an insoluble purple dye (diformazan). The dye is visible at areas where the labeled probe has hybridised to its target.

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Folin & Ciocalteu's phenol reagent is most commonly used in the Lowry method for determining protein concentration. It has also been used for the quantification of total phenolics. In this method, protein is pretreated with copper(II) in a modified biuret reagent (alkaline copper solution
stabilized with sodium potassium tartrate). Addition of the phenol reagent generates chromogens that give increasing absorbance between 550-750nm. Normally, absorbance at the peak (750 nm) or shoulder (660 nm) are used to quantitate protein concentrations between 1 - 100 mg/ml while absorbance at 550 nm is used to quantitate higher protein concentrations.

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The polycyclic aromatic hydrocarbon perylene is a hydrophobic fluorescent probe. It is highly photostable and displays blue fluorescence. Perylene is used as a dye for spectroscopic studies.

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Carbol Fuchsin is a basic dye which forms a yellow-brown compound in presence of acid. It is mainly required for the staining of mycobacteria. The waxy walls of mycobacteria helps in retaining the dye within cells. It can also be used for the staining of bacterial spores.

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When mixed with a 4% sodium nitrite solution, a freshly hexazotized pararosaniline solution is produced. May be used in a variety of cytochemistry procedures, including Sigma Procedures 181B and 181C.

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Homovanillic acid has been used as a standard in HPLC analysis. Metabolite of dihydroxyphenylacetic acid (DOPAC) by catechol O-methyltransferase; reagent for the fluorimetric determination of glucose oxidase and other oxidative enzymes.

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Ethidium bromide monoazide. ≥95% (HPLC), solid (3-Amino-8-azido-5-ethyl-6-phenylphenanthridinium bromide; Ethidium monoazide bromide)