"PCR Mastermixes"

Supplier: Enzo Life Sciences

Rapid removal of contaminating DNA in PCR mastermixes, without reduction in PCR sensitivity.

Supplier: MP Biomedicals

Taq-&LOAD™ PCR Master Mix is an optimised 5X reaction mix for PCR containing recombinant Taq DNA polymerase, dNTPs, MgCl₂, and incubation and loading buffers. Also included are a compound increasing the density of the sample and a red/purple dye.

Supplier: G-Biosciences

Pfu DNA Polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has superior thermostability and proofreading properties compared to other thermostable polymerases. It has a molecular weight of 90 kDa and can amplify DNA targets up to 2 kb. The elongation velocity is 0,2~0,4 kb/min (70~75 °C). Pfu DNA Polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide misincorporation errors. This means that Pfu DNA Polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA Polymerase results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA Polymerase is superior for techniques that require high-fidelity DNA synthesis.

Supplier: Quantabio

qScript™ XLT One-Step RT-qPCR ToughMix® is a ready to use, highly sensitive master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates.

Supplier: Q BIOANALYTIC

Real-Time kit for the detection of Staphylococcus aureus.

Supplier: VWR Chemicals

VWR® Taq DNA Polymerase Master Mix is a ready to use 1,1X or 2X reaction mix. Simply add primers, template and water to carry out primer extensions and other molecular biology applications.

Supplier: VWR Chemicals

VWR® Red Taq DNA Polymerase Master Mix, which also contains an inert red dye, can be directly loaded onto an agarose gel without addition of electrophoresis loading buffers.

Supplier: VWR Chemicals

VWR® TEMPase Hot Start DNA Polymerases are highly stable polymerases, featuring higher specificity, superior sensitivity and greater yields compared to standard DNA polymerases. These features make them well suited for the detection of low abundance targets. Other uses include screening, amplification of GC-rich sequences, multiplex PCR, direct PCR and qPCR.

Supplier: Q BIOANALYTIC

The Real-Time PCR screening kit is addressing three different targets: 35S promoter; NOS Terminator; CTP2-CP4 EPSPS. The tests are all in accordance with the JRC guideline. Advantage of the CTP2-CP4 EPSPS compared to FMV detection is that the used target includes artificial sequences, which are not present naturally like the virus sequence of FMV.

Supplier: Q BIOANALYTIC

Vibrio alginolyticus causes eye, ear and wound infections. It can be tested using this Real-Time PCR test. The analysis can be done in water and food samples. You can use this test in combination with an appropriate pre- enrichment in alkaline peptone-water or you can confirm bacterial colonies on agar plates.

Supplier: Q BIOANALYTIC

Testing for pathogenic E. coli like EHEC, EPEC , EIEC etc. can help to prevent foodborne infections as it has taken place in 2011 with E. coli strain O104 in Germany and with strain O157 at other places in the world. The real-time PCR kit allows the detection of stx1, stx2 and the eaeA gene which covers all different strains of pathogenic E. coli. The test can also be used for the confirmation of bacterial colonies.

Supplier: Q BIOANALYTIC

Vibrio vulnificus causes severe wound infections and is harmful when ingested. 33% of patients being infected die. The detection in food and water samples using this Real-Time PCR Test is very rapid and versatile. You have results available already after 24 hours. You can use this test in combination with an appropriate pre- enrichment in alkaline peptone-water or you can confirm bacterial colonies on agar plates.

Supplier: Q BIOANALYTIC

Legionella spec. is tested usually with cultural methods. However, this may take up to 14 days to deliver results and some strains are living but are not culturable. Using this Real-Time PCR test you may have results already after a few hours. You can use this test in combination with an appropriate pre- enrichment or you can do a DNA purification directly from the filter without pre-enrichment. The kit is also suitable to confirm bacterial colonies on agar plates.

Supplier: Q BIOANALYTIC

Kit for Vibrio cholerae tox gene (ctx). Food safety experts like the German DGHM recommend to test warm water fish for vibrio cholerae. With this Real-Time PCR test you have a very versatile method for testing whether or not the present strain has the ability to produce toxins. You can use our Real-Time PCR Test for Vibrio cholerae for testing for the presence of the species before. You can use this test in combination with an appropriate pre- enrichment in alkaline peptone-water or you can confirm bacterial colonies on agar plates.

Supplier: VWR Chemicals

TEMPase Hot Start DNA Polymerase Master Mix and Blue TEMPase Master Mix are good alternatives to TEMPase Hot Start DNA Polymerase. These master mixes offer easy reaction assembly at room temperature, reduced set-up time and fewer handling steps, which lead to increased reproducibility. As a consequence TEMPase Hot Start DNA Polymerase Master Mix is highly suited to standard tests.

Supplier: Q BIOANALYTIC

Vibrio parahaemolyticus tox gene (tdh/trh): Food safety experts like the German DGHM recommend to test warm water fish for Vibrio parahaemolyticus. With this Real-Time PCR test you have a very versatile method for testing whether or not the present strain has the ability to produce toxins (tdh/trh). You can use our Real-Time PCR Test for Vibrio parahaemolyticus for testing for the presence of the species before. You can use this test in combination with an appropriate pre- enrichment in alkaline peptone-water or you can confirm bacterial colonies on agar plates.