17629 Results for: "Next+Generation+Sequencing+Instruments&pageNo=30&view=easy"

Supplier: ProSci Inc.

This antibody is specific for a 17-26 kDa protein, which is identified as the cytokine TNF alpha (Tumor Necrosis Factor alpha). It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. It is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. It exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17 kDa band following SDS PAGE under reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.

Supplier: Thermo Scientific

General Purpose Pro Centrifuges Cell Therapy Systems (CTS™) Series consist of equipment, documentation, and certifications, as well as compliance services that support Good Manufacturing Practice (GMP) requirements. The CTS Series helps you get up and running faster, stay compliant and current with all necessary regulatory certifications, navigate regulatory audits, and stay on schedule, taking cell therapy from discovery to clinical research and commercial manufacturing. The refrigerated 4 L (4×1000 ml) floor standing and bench models are designed to meet the needs of today’s rapid-fire discoveries, with updates to help get research done quickly, consistently, and reliably.

Supplier: MP Biomedicals

SPINeasy Plasmid Midiprep kit is a silica-membrane spin-column kit for the purification of high-copy and low-copy number plasmid DNA from 25 to 50 ml of transformed bacterial (E.coli) culture.

Supplier: Cytiva

Sephadex™ G-50 M DNA Grade chromatography resin for purification of DNA fragments up to 20 bases in length from small molecules such as salts by size exclusion.

Supplier: MP Biomedicals

SPINeasy Plasmid Miniprep kit is a silica-membrane spin-column kit that enables up to 20 µg of plasmid DNA to be purified from 1 to 5 ml of transformed bacterial culture.

Supplier: ProSci Inc.

This antibody is specific to a conserved determinant of the p53 protein. PAb 122 binds to the C-terminus (aa 370-378) of both wild type and mutated p53. When microinjected into nuclei, PAb 122 blocked re-entry into the S-phase of the cell cycle. Mutation and/or allelic loss of p53 is one of the causes of a variety of mesenchymal and epithelial tumors. If it occurs in the germ line, such tumors run in families. p53 binds to a DNA consensus sequence, the p53 response element, and it regulates normal cell growth cycle events by activating transcription of genes, involved either in progression through the cycle, or causing arrest in G1 when the genome is damaged. In most transformed and tumor cells the concentration of p53 is increased 5-1000 fold over the minute concentrations (1000 molecules cell) in normal cells, principally due to the increased half-life (4 h) compared to that of the wild-type (20 min). The protein in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution. Its the most commonly mutated gene in spontaneously occurring human cancers. Mutations arise with an average frequency of 70% but incidence varies from zero in carcinoid lung tumors to 97% in primary melanomas. High concentrations of p53 protein are transiently expressed in human epidermis and superficial dermal fibroblasts following mild ultraviolet irradiation.

Supplier: MP Biomedicals

FastRNA™ Pro Soil-Direct Kit is used to isolate total RNA from organic material found in soil samples. The total RNA isolated is suitable for RT-PCR analysis and other downstream applications.

Supplier: SI Analytics

These very compact, easy to operate instruments are ideal for coulometric water determination according to Karl Fischer in low water content, as well as for bromine number determination. They are suitable for small liquid or gaseous sample amounts, and can be used for quality control in the chemical, petrochemical, pharmaceutical and food industries.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® DNA/RNA isolation kit is designed for the simultaneous isolation of both genomic DNA and total RNA from the same cells or tissues. The sample is first lysed and homogenised in a special denaturing buffer, spun to pellet RNA and undigested particles, and then the supernatant is applied to a HiBind® DNA spin column to bind DNA. The RNA pellet is dissolved and purified by a HiBind RNA spin column. Since there is no need to divide the sample into two parts for separate purification procedures, maximum yield of DNA and RNA can be purified from the entire sample.

Supplier: MP Biomedicals

SPINeasy® DNA Kit for Microbiome employs a novel method for isolating microbial genomic DNA from various samples. Due to its highly effective lysis capability and silica-membrane spin-column technology, the SPINeasy® DNA Kit for Microbiome can be used for bacterial, body fluids and environmental samples such as soil and stool.

Supplier: Cytiva

Sephadex G-50 grade F is a size exclusion chromatography resin designed for desalting and buffer exchange, and for purification of DNA and oligonucleotides greater than 20 bases in length using spin columns.

Supplier: MP Biomedicals

Host DNA contamination impedes the molecular analyses of microbiomes in host samples, such as bodily fluids and swabs. The SPINeasy Host Depletion Microbial DNA kit provides an easy-to-use workflow to isolate microbial DNA from samples containing high amounts of host DNA.

Supplier: Merck Millipore (Novagen)

The SpinPrep™ Gel DNA Kit enables efficient extraction of DNA fragments of 150 bp to >12000 bp in size from agarose gels. The procedure uses GelMelt™ solution to dissolve the gel slice, followed by adsorption of the DNA to a silica membrane in a spin column format. After a wash step, the purified DNA is eluted in low salt buffer. Each spin column can bind up to 20 µg DNA. Recoveries are consistently in the range of 50 to 90% under standard conditions.

Supplier: HUBER

Their compact dimensions enable Ministats® to be placed in the smallest of spaces, for example in a laboratory fume cupboard or inside technical plant. Despite their minimal size the systems have a wide variety of features and offer performance levels suitable for temperature control of photometers, refractometers, viscometers, distillation apparatus, reaction vessels and mini-plants. They are mainly used for external applications, however the bath opening allows objects to be thermoregulated directly in a thermostat bath. A powerful pressure/suction pump with an infinitely controlled rotational speed ensures optimum circulation. The maximum pressure can also be regulated by an optional pressure sensor, meaning that sensitive glass reactors and apparatus can be protected from breakage. Ministats® have the 'professional' level Pilot ONE® controller as standard with a colour TFT display and convenient operator guidance. Analogue NAMUR connections are available via the optional Com.G@te module allowing Ministats® to be integrated into plant and process control systems. The Pilot ONE® multi-touch controller features a brilliant 14,5 cm touchscreen with graphic functions and easy navigation menu in 11 languages. All important operating parameters and temperature values are clearly displayed on the touchscreen. The favourites menu, one-click operation and the integral technical glossary mean operation is simple. Integrated USB and Ethernet ports allows connection to a PC or network, e.g. for remote control or data transmission.

Supplier: Quantabio

Extracta™ DBS is a ready to use DNA extraction reagent for rapid and efficient recovery of PCR-ready DNA from dried blood spots (DBS) on Guthrie cards or Whatman® 903 filter/TFN or 226 from Ahlstrom Munksjö paper. This patented single-solution process produces DNA eluates that are substantially free of PCR inhibitors and compatible with a variety of end-point PCR, real-time PCR and Next Generation Sequencing (NGS) or Sanger Sequencing reagents. Application of Extracta DBS with PerfeCTa® qPCR ToughMix® or PerfeCTa MultiPlex qPCR ToughMix® enables accurate and reproducible quantification of DNA sequences in blood using TaqMan® hydrolysis probe real-time qPCR.

Supplier: HAMILTON ROBOTICS

Hamilton CO-RE tips were designed for MICROLAB pipetting workstations in order to solve the problem of sealing and alignment plagued by press-fit tip attachment techniques. Each CO-RE disposable tip has a circular groove inside the attachment orifice. An o-ring on the pipetting channel is expanded to fit this groove precisely providing an air-tight seal and straight alignment between tip and pipetting channel.

Supplier: GRAM

BioLine is a comprehensive refrigeration range designed to deal with special bioscience needs. BioLine is distinct from other 'spark-free' refrigerators on the market, as both the interior and the exterior of all units have been shown to comply with the European ATEX [EN/IEC60079-15] directive which means they are safe for use in potentially explosive atmospheres (Zone 2 [Category 3]). The unique air distribution system combined with a finned tube evaporator results in excellent temperature consistency for biostorage; the evaporator design eliminates the risk of 'cold walls', which can damage delicate items stored touching the sides of the cabinet. Whilst the air distribution system which directs the cold air down the rear of the cabinet and back up to the evaporator fan at the top, ensures a uniform temperature throughout - using only a minimum of energy. The use of both cyclopentane to foam the insulation material and also hydrocarbon refrigerants means that BioLine storage solutions have only minimal environmental impact.

Supplier: GRAM

BioLine is a comprehensive refrigeration range designed to deal with special bioscience needs. BioLine is distinct from other 'spark-free' freezers on the market, as both the interior and the exterior of all units have been shown to comply with the European guidelines contained in the ATEX [EN/IEC60079-15] directive which means they are safe for use in potentially explosive atmospheres (Zone 2 [Category 3]). The unique air distribution system is combined with BioLine’s innovative 'smart defrost' function to maintain temperature consistency for biostorage: Smart defrost is an intelligent automatic defrosting control that makes sure the absolute minimum of time and energy is used during each defrost cycle. Whilst the air distribution system which directs the cold air down a special plate to the rear of the cabinet interior and back up to the evaporator fan mounted in the top, ensures a uniform temperature throughout using only a minimum of energy. And by also using cyclopentane to foam the insulation material and hydrocarbon refrigerants, BioLine storage solutions have only minimal environmental impact.

Supplier: Merck

The next generation membrane dispenser: Fast, robust, no-touch dispensing.