"MP Biomedicals"

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Actin Monoclonal Antibody was used for western blot analysis of Cos-7 cell lysates to ensure equal protein loading. Western blot analysis of MDCK cell lysates were performed using monoclonal anti-actin antibody as a primary antibody.It is also used in indirect ELISA, immunofluorescence, immunoblots and immunohistochemistry

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Rifamycin SV is a broad-spectrum semisynthetic antimicrobial agent of the rifamycin group.

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Soluble in water (50 mg/ml) clear,colourless solution.

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Ammonium tartrate, the ammonium salt of tartaric acid, is used in such applications as cell culture and chromatography.

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Suitable for both protein and nucleic acid isolation. Exhibits proteolytic activity on proteins, peptides, glycoproteins, amides and esters. Also active with nitroanilides of amino acids with protected amino groups, excluding arginine. Useful in the isolation of DNA and RNA, in the analysis of membrane structures and protein structure. Proteolytic activity: >30mAnson Units/mg Unit definition: One Anson unit is the amount of enzyme which liberates 1 µmol of Folin-positive amino acids per minute at pH 7,5 and 35 °C, using hemoglobin as substrate.

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Iodoacetamide is a thiol reagent; alkylating reagent for cysteine and histidine residues in proteins. In the alkylation reaction, it reacts with histidine (such as in RNase), methionine and sulfhydryl groups of many proteins.

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L-Malic Acid is a dicarboxylic acid that is made by all living organisms. The malate anion is an intermediate in the citric acid cycle.

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Fluorescein diacetate

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Easily soluble in water. The aqueous solution is unstable and can be used only for a few days even if it is kept in a refrigerator. 1% aqueous solution is dark and turns reddish when exposed to light.

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Brilliant Cresyl Blue is a supravital stain used in hematology for demonstrating and counting reticulocytes and platelets. It is also used as a vital stain for demonstrating the mucous secretions of the marine coelomates.

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Adenosine 5'-monophosphate disodium salt (AMP disodium salt) hydrate ≥99%, powder

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Tannic Acid is used for enzyme immobilization and protein adsorption

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L-Methionine

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Linolenic acid belongs to the class of omega-3 fatty acids.

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Applications
Guanidine hydrochloride can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Also used in the isolation of RNA. It is a strong chaotropic agent useful for the denaturation and subsequent refolding of protein, it can solubilize insoluble or denatured proteins such as inclusion bodies and be used for the recovery of periplasmic proteins. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Also used in the isolation of RNA.
Product Description
The crystal structure of Guanidine hydrochloride consists of a network of guanidinium cations and chloride anions linked by N–H•••Cl hydrogen bonds,it is a strong chaotropic agent.Guanidine HCl may agglomerate upon storage. It may appear as a free-flowing crystalline powder, a freeflowing powder with solid material dispersed throughout, or a solid. The quality of the product does not appear to be affected and solutions prepared from the free-flowing and lumpy guanidine HCl appear identical

Grade: Ultra Pure
Purity: >99%
Keywords: Guanidium chloride, chaotropic agent
Key Applications: Chaeotropic agent
Product Type: Biochemicals
Biochemical Category: Chaotropic Agents
Density: 1.345 g/cm³ at 20 °C (Lit.)
Melting Point: 180-190 °C
UV/Visible Absorbance: OD260nm (6.0 M aq soln) <0.03
Presentation: White Crystalline Powder
pH: 4-6 (6.0 M aq soln)
Solubility: Soluble in water,Clear, Colorless Solution (6 M - clear, colorless solution).
Storage & Handling: Room Temperature, desiccate

Supplier: MP Biomedicals

o-Nitrophenyl-β-D-galactoside is a cell culture component for molecular genetics. It is chromogenic substrate for b-galactosidase and hence is useful in the detection of lacZ activity.