9322 Results for: "Luff-Schoorl\'s+reagent"

Supplier: FINNZYMES REAGENTS

Master Mix Phire Hot Start II PCR 200x50µl reactions 1 * 1 KIT

Supplier: Palintest

COPPER TEST TUBES 0-20 mg PL427 1 Box = 25 tubes 1 * 1 Each

Supplier: Cytiva

MabSelect SuRe™ is composed of a rigid, high-flow agarose matrix and alkali-tolerant rProtein A ligand. The ligand has been engineered to provide greater stability than conventional protein A-based media in the alkaline conditions used in CIP protocols. This enhanced alkali stability improves process economy and product quality.

Supplier: FINNZYMES REAGENTS

Master Mix Phire Green Hot Start II PCR 200x50µl reactions 1 * 1 KIT

Supplier: FINNZYMES REAGENTS

Kit Master Mix PCR Phire Plant Direct 100 x 50 µl rxns 1 * 1 KIT

Supplier: Thermo Orion

RETROFIT KIT FOR 1811EL SODIUM ANALYSER 1 * 1 KIT

Supplier: FINNZYMES REAGENTS

Reaction Buffer Phire Green 4x1,5ml 1 * 1 KIT

Supplier: FINNZYMES REAGENTS

Kit Master Mix PCR Phire Plant Direct 500 x 50 µl rxns 1 * 1 KIT

Supplier: FINNZYMES REAGENTS

Phusion plus dna polymerase 4x500 rxn 1 * 2.000 Tests

Supplier: FINNZYMES REAGENTS

Buffer pack PCR Phusion High-Fidelity final 1x buffer concentration equals 1,5mM MgCl2, contains 4x1,5ml of 5x buffer, 2x1,5ml of 50 mM MgCl2 solution, 1x0,5ml of 100% DMSO 1 * 1 KIT

Supplier: FINNZYMES REAGENTS

Buffer pack PCR Phire reaction final 1x buffer concentration equals 1,5mM MgCl2, contains 4x1,5ml of 5x buffer, 2x1,5ml of 50mM MgCl 2 solution, 1x0,5ml of 100% DMSO 1 * 1 KIT

Supplier: FINNZYMES REAGENTS

Buffer pack PCR Phusion GC final 1x buffer concentration equals 1,5mM MgCl2, contains 4x1,5ml of 5x buffer, 2x1,5ml of 50mM MgCl2 so lution, 1x0,5ml of 100% DMSO 1 * 1 KIT

Supplier: Honeywell Chemicals

Dichloromethane Laboratory Reagen (4X5L) 1 * 20 L

Supplier: FINNZYMES REAGENTS

Master Mix Phire Green Hot Start II PCR 1000x50µl reactions 1 * 1 KIT

Supplier: Hach

MEL/MPN Coliform Laboratory Consumables Replacement Set, pk/1, incl. 50 presterilized inoculating loops, 50 presterilized 10-mL pipe ts, and 100 presterilized Whirl-Pak Bags, Media must be ordered separately 1 * 1 SET

Supplier: FINNZYMES REAGENTS

Master Mix Phire Hot Start II PCR 1000x50µl reactions 1 * 1 KIT

Supplier: Palintest

TOTAL HARDNESS LR 0-50mg CacO3 50 tests 1 * 50 items

Supplier: MP Biomedicals

Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C

Supplier: BIOPREMIER

Food safe kits provide a simple, reliable, and rapid procedure for detecting the presence of an animal species in meat and meat products. The assay is based on 5’ nuclease Real-Time PCR reactions to amplify a unique genomic sequence in the target organism. According to European Commission Directive 2002/86/EC, food ingredients have to be declared. In the case of meat and meat products, the animal species has to be disclosed on the label. Moreover, the species autenticity can be highly relevant to consumers for economic, medical, cultural and religious reasons. Fraudulent substitution of cheaper meats in place of more expensive species, the inclusion of meat in non meat (vegetarian) products and the presence of allergens in food products are clear examples for the importance of this issue (Ballin, 2010; Dooley et al., 2004). Several methods are based on identification of proteins by means of electrophoretic and/or immunological methods. However, these methods are not reliable for application in highly processed and heated products due to the protein deterioration. DNA is more stable than proteins during processing and although it can be fragmented by several processes, modern DNA methodologies like PCR-based techniques still allow the identification of DNA from the different species present in a sample (Lockley & Bardsley, 2000). Real-Time PCR techniques are especially suitable for these products because small fragments of DNA can still be amplified and identified with high sensitivity and specificity (Dooley et al., 2004).

Supplier: FINNZYMES REAGENTS

Buffer pack PCR Phusion GC detergent-free final 1x buffer concentration equals 1,5mM MgCl2, contains 4x1,5ml of 5x buffer, 2x1,5ml o f 50mM MgCl2 solution, 1x0,5ml of 100% DMSO 1 * 1 KIT

Supplier: Merck Millipore (Novagen)

Phosphosafe Extraction Buffer 1 * 25 mL

Supplier: Merck Millipore (Calbiochem‎)

Seablock Salmon Plasma 1 * 500 mL

Supplier: FINNZYMES REAGENTS

Buffer pack PCR Phire reaction detergent-free final 1x buffer concentration equals 1,5mM MgCl2, contains 4x1,5ml of 5x buffer, 2x1,5 ml of 50mM MgCl2 solution, 1x0,5ml of 100% DMSO 1 * 1 KIT

Supplier: FINNZYMES REAGENTS

Buffer pack PCR Phusion High Fidelity detergent-free final 1x buffer concentration equals 1,5mM MgCl2, contains 4x1,5ml of 5x buffer , 2x1,5ml of 50mM MgCl2 solution, 1x0,5ml of 100% DMSO 1 * 1 KIT

Supplier: FINNZYMES REAGENTS

Kit qPCR DyNAmo HS SYBR Green 200x50µl reactions (2x Master Mix contains a hot start version of a modified Thermus brockianus DNA po lymerase, SYBR Green I dye, optimized PCR buffer, 5 mM MgCl2, dNTP mix including dUTP), vial of 50x ROX passive refer 1 * 1 KIT

Supplier: MARKET SOURCE PART PROCESS

INCUCYTE® CYTOLIGHT RAPID DYE FOR LIVE-CELL CYTOPLASMIC LABELING RED DYE 1 * 5 Vial

Supplier: FINNZYMES REAGENTS

Kit Mutation Generation (MGS) generation of random insertions 10 reactions. Kit includes MuA Transposase (10l), Entranceposon (M1-C amR) (10l), Entranceposon (M1-KanR) (10l), 5x reaction buffer for MuA (100l), control target DNA (10l), NotI Mini 1 * 1 KIT

Supplier: MACHEREY-NAGEL

Acidity AC 7 Refill pack VISOCOLOR HE. 1 * 1 KIT

Supplier: FINNZYMES REAGENTS

Kit qPCR DyNAmo HS SYBR Green 1000x50µl reactions (2x Master Mix contains a hot start version of a modified Thermus brockianus DNA p olymerase, SYBR Green I dye, optimized PCR buffer, 5 mM MgCl2, dNTP mix including dUTP), vial of 50x ROX passive refe 1 * 1 KIT

Supplier: FINNZYMES REAGENTS

Kit Template Generation System II (TGS II) generation of primer binding sites 20 reactions. Kit includes MuA Transposase (20l), 5x reaction buffer for MuA (100l), control target DNA (10l), Entranceposon (CamR-3) (20l), Entranceposon (KanR-3) (20 1 * 1 KIT