67541 Results for: "Glass+Fibre+Filters"
Pierce™ DNA Extraction Reagent Kit, Yeast
Supplier: Thermo Fisher Scientific
The Pierce™ yeast DNA extraction reagent kit uses Y-PER yeast protein extraction reagent to quickly extract and purify genomic and plasmid DNA from yeast.
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Urea ≥98%, white powder for molecular biology
Supplier: MP Biomedicals
Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C
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L(+)-Cysteine hydrochloride monohydrate
Supplier: PanReac AppliChem
L(+)-Cysteine hydrochloride monohydrate
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Sodium deoxycholate monohydrate ≥98%
Supplier: Thermo Fisher Scientific
Sodium deoxycholate monohydrate ≥98%
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E.Z.N.A.® Universal Pathogen DNA Kit
Supplier: OMEGA BIO-TEK
The E.Z.N.A.® Universal Pathogen Kit allows for the rapid and reliable isolation of high quality host genomic DNA, gram positive and negative bacterial DNA, fungal spore DNA and viral DNA and viral RNA from tissue, urine, serum and fecal samples.
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di-Sodium L(+)-tartrate dihydrate ≥99%
Supplier: Thermo Fisher Scientific
di-Sodium L(+)-tartrate dihydrate ≥99%
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EZ Yeast™ Plasmid Prep kit
Supplier: G-Biosciences
The EZ Yeast™ Plasmid Prep Kit is designed for the rapid isolation of plasmids from yeast patches or yeast grown in small liquid cultures. The kit is adapted from the alkaline lysis of E. coli, by providing modified alkaline lysis buffers and LongLife™ Zymolyase®, a highly stable enzyme.
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CO₂ incubators, ICO series
Supplier: MEMMERT
The ICO series of CO₂ incubators has seamlessly welded, corrosion-resistant stainless steel chambers with rounded edges and no further installations, ensuring easy and thorough cleaning. Models have TwinDISPLAY with a USB port for uploading programs, reading out logs or protecting appliances via the user ID function, and an Ethernet interface as well as a data logger with a 10 year storage capacity. All parameters can be set directly in the ControlCOCKPIT or the AtmoCONTROL software, which uses drag and drop symbols to input values. Battery-buffered ControlCOCKPIT (optional): Operating display, logging and CO₂ control are fully functional even during a power failure.
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L(-)-Glutathione (reduced form) ≥98%, off-white powder cell culture reagent
Supplier: MP Biomedicals
Storage: Store at +4 °C, store under nitrogen
Glutathione is the major low molecular weight thiol compound of the living plant or animal cell. It is a tripeptide with a gamma peptide linkage between the amine group of cysteine (which is attached by normal peptide linkage to a glycine) and the carboxyl group of the glutamate side-chain. It is an antioxidant, preventing damage to important cellular components caused by reactive oxygen species such as free radicals and peroxides. The sulfhydryl (thiol) group (SH) of cysteine serves as a proton donor and is responsible for the biological activity of glutathione.
Glutathione suppresses human immunodeficiency virus expression in chronically infected monocytic cells. It is a useful tripeptide involved in many aspects of metabolism, including transport of g-glutanyl amino acids and reductive cleavage of disulfide bonds.
Endogenous antioxidant that plays a major role in reducing reactive oxygen species formed during cellular metabolism and the respiratory burst. Glutathione-S-transferase catalyzes the formation of glutathione thioethers with xenobiotics, leukotrienes, and other molecules that have an electrophilic center. Glutathione also forms disulfide bonds with cysteine residues in proteins. Via these mechanisms, it can have the paradoxical effect of reducing the efficacy of anti-cancer agents.
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HERAcell® i CO₂ Incubators
Supplier: Thermo Scientific
HERAcell® i series CO₂ incubators provide stable, accurately monitored growth conditions offering excellent protection against contamination for valuable cell and tissue cultures. Extremely short CO₂ and temperature recovery times enhance cell growth. They are available in two practical sizes and two inner chamber designs; non-corrosive stainless steel or 100% antimicrobial solid copper (to verifiably prevent the growth of bacteria and fungi by natural means). As standard, models are supplied with high quality thermal conductivity (TC) sensors.
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Dipotassium-L(+)-tartrate hemihydrate ≥99%
Supplier: Thermo Fisher Scientific
Dipotassium-L(+)-tartrate hemihydrate ≥99%
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Zirconyl chloride octahydrate ≥98%
Supplier: Thermo Fisher Scientific
Zirconyl chloride octahydrate ≥98%
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Freezers
Supplier: GRAM
The unique air distribution system combined with a finned tube evaporator results in excellent temperature consistency for biostorage: the evaporator design eliminates the risk of 'cold walls', which can damage delicate items stored touching the sides of the cabinet. Whilst the air distribution system which directs the cold air down the rear of the cabinet and back up to the evaporator fan at the top, ensures a uniform temperature throughout.
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CO₂ incubators, ICOmed series
Supplier: MEMMERT
The ICOmed series of CO₂ incubators has seamlessly welded, corrosion-resistant stainless steel chambers with rounded edges and no further installations, ensuring easy and thorough cleaning. Models have TwinDISPLAY with a USB port for uploading programs, reading out logs or protecting appliances via the user ID function, and an Ethernet interface as well as a data logger with a ten year storage capacity. All parameters can be set directly in the ControlCOCKPIT or the AtmoCONTROL software, which uses drag and drop symbols to input values. Battery-buffered ControlCOCKPIT (optional): Operating display, logging and CO₂ control are fully functional even during a power failure.
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L(+)-Potassium sodium tartrate tetrahydrate 99.0-102.0% ACS
Supplier: Thermo Fisher Scientific
L(+)-Potassium sodium tartrate tetrahydrate 99.0-102.0% ACS
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Vacuum drying chambers, VD and VDL series
Supplier: Binder
The VD series of vacuum drying chambers provide fast gentle drying of non flammable solvents, delivering impressive performance through their precise temperature control and gentle drying. Expansion rack technology also ensures optimal heat transfer, shelves provide flexible placement, and the interior of the chamber is easy to clean. The VDL series of safety vacuum drying chambers ensures maximum safety when drying flammable organic solvents, and have been safety tested by a state recognised test institute (GS mark). VD/VDL units can also be purchased as modular options with vacuum pump and pump chamber to form a complete system.
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β-Nicotinamide adenine dinucleotid, oxidised form (NAD, oxidised form) ≥98%, white powder cell culture reagent
Supplier: MP Biomedicals
Storage: -20°C, desiccate
This is an ultrapure NAD, chromatographically purified to remove trace inhibitors.
β-NAD, a pyridine nucleotide and biologically active form of nicotinic acid, is a coenzyme necessary for the catalytic reaction of certain enzymes. It occurs in living cells primarily in the oxidized state. Serves as a coenzyme of the dehydrogenases, especially in the dehydrogenation of primary and secondary alcohols. NAD usually acts as a hydrogen acceptor, forming NADH which then serves as a hydrogen donor in the respiratory chain.
Many metabolites and enzymes of biological interest are present in tissues at low concentrations. With the use of β-NAD as a catalyst intermediate and several enzymes in a multistep system, known as enzyme cycling, much greater sensitivity for detection of these components is achieved. The reduced form, β-NADH, is fluorescent whereas β-NAD is not. This difference in fluorescence provides a sensitive fluorescent measurement of the oxidized or reduced pyridine nucleotides at concentrations down to 10-7 M.
Electron acceptor. β-NAD is a carrier for hydride ion, forming b-NADH. Hydride ion is enzymatically removed from a substrate molecule by the action of dehydrogenases such as malic dehydrogenase and lactic dehydrogenase. Such enzymes catalyze the reversible transfer of a hydride ion from malate or lactate to b-NAD to form the reduced product, b-NADH. Unlike b-NAD which has no absorbance at 340 nm, b-NADH absorbs at 340 nm (EmM = 6.22). The increase in absorbance at 340 nm with the formation of b-NADH is the basis for measurement of activity of many enzymes.
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Electroelution device for extracting nucleic acids and proteins, GeneCAPSULE™ (G-CAPSULE™)
Supplier: G-Biosciences
G-Biosciences' GeneCAPSULE™ is an electro-elution tool for rapid, nearly 90% recovery of PCR products, DNA fragments and proteins from agarose and polyacrylamide gels
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L(+)-Potassium sodium tartrate tetrahydrate ≥99%
Supplier: Thermo Fisher Scientific
L(+)-Potassium sodium tartrate tetrahydrate ≥99%
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L(+)-Histidine monohydrochloride monohydrate
Supplier: PanReac AppliChem
L(+)-Histidine monohydrochloride monohydrate
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Tissue Genomic DNA Extraction Kits
Supplier: BIOPREMIER
The tissue genomic DNA extraction Kit is designed for the rapid purification of highly pure genomic DNA from a wide variety of samples, including whole blood, culture cells, animal tissues, mouse tails, bacteria, yeats, clinic samples (serum, plasma, feces, orine), forensic samples and paraffin-embedded tissues.
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Zirconyl chloride octahydrate 98%
Supplier: Thermo Fisher Scientific
Zirconyl chloride octahydrate 98%
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L(+)-Cysteine hydrochloride monohydrate ≥99%
Supplier: Thermo Fisher Scientific
L(+)-Cysteine hydrochloride monohydrate ≥99%
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L(+)-Histidine monohydrochloride monohydrate
Supplier: PanReac AppliChem
L(+)-Histidine monohydrochloride monohydrate
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L(+)-Cysteine hydrochloride monohydrate
Supplier: EDQM
L(+)-Cysteine hydrochloride monohydrate
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illustra™ GFX™ PCR DNA and gel band purification kit
Supplier: Cytiva
The illustra GFX PCR DNA and Gel Band Purification kits are for the isolation and concentration of DNA fragments from PCR mixtures, DNA-containing agarose gel bands, enzyme-based DNA modifications, and restriction enzyme digests.
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Antimony(III) potassium oxitartrate trihydrate 99+%
Supplier: Thermo Fisher Scientific
Antimony(III) potassium oxitartrate trihydrate 99+%
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Antimony(III) potassium oxitartrate trihydrate 99.0-103.0% ACS
Supplier: Thermo Fisher Scientific
Antimony(III) potassium oxitartrate trihydrate 99.0-103.0% ACS