Supplier: G-Biosciences

CB™ Protein Assay, a Coomassie dye based protein assay based on the Bradford Protein Assay, is suitable for the simple and rapid estimation of protein concentration. The binding of protein to the dye results in a colour change from brown to blue. The change in colour density is proportional to protein concentration. Protein estimation can be performed using as little as 0,5 µg protein.

Supplier: Biotium

TUNEL (terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick-end labeling) is highly selective for the detection of apoptotic cells, but not necrotic cells or cells with DNA strand breaks resulting from irradiation or drug treatment. In this assay, TdT enzyme catalyses the addition of labeled dUTP to the 3’ ends of cleaved DNA fragments. Fluorescent dye-conjugated dUTP can be used for direct detection of fragmented DNA by fluorescence microscopy or flow cytometry.

Supplier: ENZO LIFE SCIENCES

The assay is designed to detect ADP/ATP ratio. The changes in ADP/ATP ratios have been used to conveniently differentiate apoptosis from necrosis.

Supplier: ENZO LIFE SCIENCES

Simple assay to quantify senescence-associated β-galactosidase activity, in live cells, using flow cytometry or fluorescent microscopy.

Supplier: ENZO LIFE SCIENCES

The DPPIV/CD26 Assay kit for biological samples is a complete assay system designed to measure DPPIV activity in biological fluids such as plasma, serum, urine, and saliva. Uses for the kit include correlation of DPPIV activity with disease states or determination of the efficacy of DPPIV inhibitors administered in vivo.

Supplier: Cayman Chemical

Neutrophil/Monocyte Respiratory Burst Assay Kit provides PMA, dihydrorhodamine 123, and additional reagents necessary for inducing and quantifying a respiratory burst response in neutrophils and monocytes by flow cytometry. The assay can be performed on whole blood or on cells in various types of cell culture media. Because the assay reagents are not species-specific, this assay can be used in any species or cell type capable of producing a NADPH oxidase-dependent respiratory burst response.

Supplier: MOLECULAR DEVICES

The FLIPR® Calcium 4 Assay Kit provides a fast, simple and reliable fluorescence-based assay for detecting changes in intracellular calcium.

Supplier: MOLECULAR DEVICES

The FLIPR® Calcium 5 Assay Kit provides a fast, simple, and reliable fluorescence-based assay for detecting changes in intracellular calcium.

Supplier: NICKEL ELECTRO

Crucible lid, For Ø: 45 mm, Nickel

Supplier: ENZO LIFE SCIENCES

The GFP-Certified™ FluoForte™ Calcium Assay kit provides a homogeneous fluorescence-based assay for detecting intracellular calcium mobilization across a broad spectrum of biological targets.

Supplier: Cayman Chemical

HIF-1α Transcription Factor Assay Kit is a sensitive, non radioactive method for detecting HIF-1α from whole cell lysates.

Supplier: Cayman Chemical

96-Well assay for measurement of p53.

Supplier: Cayman Chemical

A 96-well assay for measurement of PPARδ DNA binding activity.

Supplier: VWR Chemicals

The Protein Precipitation Kit utilises a simple trichloroacetic acid (TCA) based method to precipitate proteins in complex mixtures. Only protein in the sample is pelleted, while interfering salts and surfactants remain in the supernatant. A gentle acetone wash removes TCA and co-precipitants allowing the end-user to recover the protein in a small volume of buffer.

Supplier: Cayman Chemical

Phagocytosis Assay Kit (IgG FITC) for the identification of factors regulating the phagocytic process in vitro.

Supplier: Biotium

Firefly luciferase is widely used as a transcriptional reporter gene for research and drug screening. The Steady-Luc™ HTS kit can be used with luminescence plate readers without sample injection systems or for high-throughput screening.

Supplier: Biotium

CellBrite kits are designed to label cell cytoplasmic membranes with a fluorescent membrane dye pre-dissolved as an optimised staining solution. By combining CellBrite membrane stains, it is possible to label multiple cell populations with distinctive colours, a capability useful for the studies of cell-cell interactions.

Supplier: ENZO LIFE SCIENCES

The PROTEOSTAT® PDI assay kit provides a simple, homogenous assay for screening modulators of PDI enzymatic activity. This is accomplished by monitoring PDI-catalyzed reduction of insulin in the presence of Dithiothreitol (DTT), resulting in the formation of insulin aggregates which then bind avidly to the red-emitting fluorogenic PROTEOSTAT® PDI detection dye.

Supplier: G-Biosciences

This Protease Assay Kit is designed for the quantitative determination of proteases present in protein sample, using a dye-labelled protein substrate. The proteases present in the sample of interest will digest the protein substrate and release dye labelled peptides. The absorbance of the dye-labelled peptide is measured at 570 nm for determination of protease activity.

Supplier: Cayman Chemical

Triglyceride Colorimetric Assay Kit is a simple, reproducible and sensitive tool for assaying triglycerides in plasma, serum, cell lysates and tissue homogenate samples.

Supplier: MOLECULAR DEVICES

The FLIPR® Calcium 6 and 6-QF Assay Kits provide a fast, simple, and reliable fluorescence based assay for detecting changes in intracellular calcium. The Calcium 6 Assay Kit significantly reduces fluorescence background with a one-step protocol. The Calcium 6-QF Assay Kit provides a quench-free option for sensitive targets or multiplexing applications.

Supplier: ENZO LIFE SCIENCES

This E3 ligase auto-ubiquitinylation kit enables proteins to be tested for ubiquitin E3 ligase activity through assessment of their ability to under auto-ubiquitinylation. Utilizing the first three steps in the ubiquitin cascade the kit facilitates ubiquitinylation of known or putative E3 ligase enzymes followed by Western blot analysis using the highly sensitive reagents provided or using antibodies to the specific protein of interest (user supplied). A high integrity ubiquitin E3 ligase enzyme is also provided for use as a positive control.

Supplier: ENZO LIFE SCIENCES

For the quantitative determination of sPLA2 in culture supernatants, cell lysates, and buffers from any species.

Supplier: ENZO LIFE SCIENCES

CYTO-ID can monitor autophagy in live cells and autophagic flux in lysosomally inhibited live cells using a novel dye that selectively labels accumulated autophagic vacuoles.

Supplier: ENZO LIFE SCIENCES

BIOMOL® Green provides a simple and convenient method for colorimetric phosphate quantitation. It is suitable for applications include assays for phospholipid phosphatases, tyrosyl-tRNA synthetase (coupled with pyrophosphatase) and a viral RNA triphosphatase. It is most widely applied to protein phosphatase assays.

Supplier: Biotium

Conjugates for probing cAMP receptors or measuring cGMP in immunoassays.

Supplier: Biotium

Calcein-AM is a non-fluorescent, membrane permeable compound. Esterase activity in the cytoplasm of viable cells converts calcein-AM to the green fluorescent, membrane impermeant compound calcein, which is retained in viable cells with intact plasma membranes. The Viability/Cytotoxicity Assay kit for Animal Live & Dead Cells pairs calcein-AM with the vital dye ethidium homodimer III for quantitation of live and dead cells.

Supplier: G-Biosciences

Protein assay, Bovine Serum Albumin Standard (2 mg/ml)

Supplier: ENZO LIFE SCIENCES

HIGHDEF® black IHC chromogen (HRP) is intended to be used in conjunction with horseradish peroxidase (HRP)-based immunostaining or in situ hybridisation systems.

Supplier: ENZO LIFE SCIENCES

Based on the dye cresyl violet and the caspase 3/7 DEVD peptide recognition sequence, this kit allows the detection of caspases 3 and 7 in living cells by fluorescence microscopy.