22117 Results for: "End-point+PCR+Enzymes+and+Kits&pageNo=15&view=list"

Supplier: Cytiva

illustra™ tissue and cells genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high quality genomic DNA from a variety of animal tissues and mammalian cell cultures. The kit can be used for several downstream applications including restriction enzyme analysis, ligation, cloning, DNA sequencing and PCR.

Supplier: asecos

Fireproof safety cabinets, classified type 90 (fire resistant up to 90 minutes), safe, durable, robust and convenient with transport base and bicoloured cabinet design. The PEGASUS line is nearly identical to the CLASSIC line, but with integrated AGT (one handed operation for double door cabinets) and integrated TSA (door open arrest system and automatic door closing).

Supplier: SenseAnywhere

This smart probe featuring a digital SenseAnywhere Bus interface has been especially designed for measuring extreme temperatures in liquid substances. Thanks to its hermetic seal made of PFA, better known as Teflon®, the Hermetically Sealed Pt100 Probe has a high level of chemical resistance. The probe is thanks to its PFA insulation waterproof and easy to clean. It can withstand aggressive substances, allowing it to take measurements in virtually all liquid fluids while being constantly submerged. The PT100 element has a Class A accuracy; measurements are provided at a very high accuracy level.

Supplier: Bioss

Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors (1). Each of a very broad range of receptors specifically detects an extracellular stimulus (a photon, pheromone, odorant, hormone or neurotransmitter) while the effectors (i.e., adenylyl cyclase), which act to generate one or more intracellular messengers, are less numerous. In mammals, G protein alpha, Beta and Gamma polypeptides are encoded by at least 16, 4 and 7 genes, respectively (2-5). Most interest in G proteins has been focused on their a subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Four distinct classes of G alpha subunits have been identified; these include Gs, Gi, Gq and Ga 12/13 (3,4). The Gi class comprises all the known a subunits that are susceptible to pertussis toxin modifications, including Ga i-1, Ga i-2, Ga i-3, Ga o, Ga t1, Ga t2, Ga z and Ga gust (4). Of these, the three Ga i subtypes function to open atrial potassium channels (6). Ga 16 is a member of the Gq subfamily and is expressed specifically in hematopoietic cells (7).

Supplier: Bioss

Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors (1). Each of a very broad range of receptors specifically detects an extracellular stimulus (a photon, pheromone, odorant, hormone or neurotransmitter) while the effectors (i.e., adenylyl cyclase), which act to generate one or more intracellular messengers, are less numerous. In mammals, G protein alpha, Beta and Gamma polypeptides are encoded by at least 16, 4 and 7 genes, respectively (2-5). Most interest in G proteins has been focused on their a subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Four distinct classes of G alpha subunits have been identified; these include Gs, Gi, Gq and Ga 12/13 (3,4). The Gi class comprises all the known a subunits that are susceptible to pertussis toxin modifications, including Ga i-1, Ga i-2, Ga i-3, Ga o, Ga t1, Ga t2, Ga z and Ga gust (4). Of these, the three Ga i subtypes function to open atrial potassium channels (6). Ga 16 is a member of the Gq subfamily and is expressed specifically in hematopoietic cells (7).

Supplier: MP Biomedicals

SPINeasy DNA Kit for Water is a high-performance water genomic DNA (gDNA) extraction kit based on silica-membrane spin-column technology. This kit enables quick isolation of gDNA from water in less than 30 min. Water samples are processed using our uniquely formulated Lysis Buffer W1 and Lysing Matrix E to effectively lyse various types of cells. Column W1 provided in the kit has high binding capacity and selectivity for gDNA. The combination of components in the kit extracts gDNA of high yield and purity that is ready for downstream analyses such as PCR, restriction digestion and sequencing.

Supplier: Bioss

Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors (1). Each of a very broad range of receptors specifically detects an extracellular stimulus (a photon, pheromone, odorant, hormone or neurotransmitter) while the effectors (i.e., adenylyl cyclase), which act to generate one or more intracellular messengers, are less numerous. In mammals, G protein alpha, Beta and Gamma polypeptides are encoded by at least 16, 4 and 7 genes, respectively (2-5). Most interest in G proteins has been focused on their a subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Four distinct classes of G alpha subunits have been identified; these include Gs, Gi, Gq and Ga 12/13 (3,4). The Gi class comprises all the known a subunits that are susceptible to pertussis toxin modifications, including Ga i-1, Ga i-2, Ga i-3, Ga o, Ga t1, Ga t2, Ga z and Ga gust (4). Of these, the three Ga i subtypes function to open atrial potassium channels (6). Ga 16 is a member of the Gq subfamily and is expressed specifically in hematopoietic cells (7).

Supplier: ERLAB CAPTAIRE

The new Captair® Smart ductless filtering hoods are designed with a simple and innovative way of communication. Smart Technology, simple and intuitive communication by light indicates the status of the hood. A simple light pattern will indicate if the airflow is compromised, if there is a fan failure or in case of a filter breakthrough.
Please complete the quote request to enable us to assist you in finding the right unit for your application.
https://uk.cmd2.vwr.com/bin/public/fc/main?f=Y5&pimid=17327608

Supplier: ERLAB CAPTAIRE

The new Captair® Smart ductless filtering hoods are designed with a simple and innovative way of communication. Smart Technology, simple and intuitive communication by light indicates the status of the hood. A simple light pattern will indicate if the airflow is compromised, if there is a fan failure or in case of a filter breakthrough.
Please complete the quote request to enable us to assist you in finding the right unit for your application.
https://uk.cmd2.vwr.com/bin/public/fc/main?f=Y5&pimid=17327607

Supplier: SCIENTIFIC INDUSTRIES ELMIS

Same great features of the Original Vortex-Genie® 2 in addition to programmability. These rugged and reliable vortex mixers have a durable motor, solid metal casing and rubber feet that prevent them 'walking'. The Vortex-Genie® Pulse is ideal for applications requiring more aggressive action than standard vortexing.

Supplier: Bioss

Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors (1). Each of a very broad range of receptors specifically detects an extracellular stimulus (a photon, pheromone, odorant, hormone or neurotransmitter) while the effectors (i.e., adenylyl cyclase), which act to generate one or more intracellular messengers, are less numerous. In mammals, G protein alpha, Beta and Gamma polypeptides are encoded by at least 16, 4 and 7 genes, respectively (2-5). Most interest in G proteins has been focused on their a subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Four distinct classes of G alpha subunits have been identified; these include Gs, Gi, Gq and Ga 12/13 (3,4). The Gi class comprises all the known a subunits that are susceptible to pertussis toxin modifications, including Ga i-1, Ga i-2, Ga i-3, Ga o, Ga t1, Ga t2, Ga z and Ga gust (4). Of these, the three Ga i subtypes function to open atrial potassium channels (6). Ga 16 is a member of the Gq subfamily and is expressed specifically in hematopoietic cells (7).

Supplier: Bioss

Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors (1). Each of a very broad range of receptors specifically detects an extracellular stimulus (a photon, pheromone, odorant, hormone or neurotransmitter) while the effectors (i.e., adenylyl cyclase), which act to generate one or more intracellular messengers, are less numerous. In mammals, G protein alpha, Beta and Gamma polypeptides are encoded by at least 16, 4 and 7 genes, respectively (2-5). Most interest in G proteins has been focused on their a subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Four distinct classes of G alpha subunits have been identified; these include Gs, Gi, Gq and Ga 12/13 (3,4). The Gi class comprises all the known a subunits that are susceptible to pertussis toxin modifications, including Ga i-1, Ga i-2, Ga i-3, Ga o, Ga t1, Ga t2, Ga z and Ga gust (4). Of these, the three Ga i subtypes function to open atrial potassium channels (6). Ga 16 is a member of the Gq subfamily and is expressed specifically in hematopoietic cells (7).

Supplier: Biosensis

Alpha synuclein is an abundant 140 amino acid neuronal protein, expressed primarily at presynaptic terminals in the central nervous system. FUNCTION: May be involved in the regulation of dopamine release and transport. Soluble protein, normally localized primarily at the presynaptic region of axons, which can form filamentous aggregates that are the major non amyloid component of intracellular inclusions in several neurodegenerative diseases (synucleinopathies). Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase 3 activation. TISSUE SPECIFICITY: Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.SUBUNIT: Soluble monomer which can form filamentous aggregates. Interacts with UCHL1. Interacts with phospholipase D and histones. SUBCELLULAR LOCATION: Cytoplasm. Membrane. Nucleus. Note=Membrane-bound in dopaminergic neurons. Also found in the nucleus. ALTERNATIVE PRODUCTS: 3 named isoforms produced by alternative splicing. Additional isoforms seem to exist.

Supplier: Bioss

Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors (1). Each of a very broad range of receptors specifically detects an extracellular stimulus (a photon, pheromone, odorant, hormone or neurotransmitter) while the effectors (i.e., adenylyl cyclase), which act to generate one or more intracellular messengers, are less numerous. In mammals, G protein alpha, Beta and Gamma polypeptides are encoded by at least 16, 4 and 7 genes, respectively (2-5). Most interest in G proteins has been focused on their a subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Four distinct classes of G alpha subunits have been identified; these include Gs, Gi, Gq and Ga 12/13 (3,4). The Gi class comprises all the known a subunits that are susceptible to pertussis toxin modifications, including Ga i-1, Ga i-2, Ga i-3, Ga o, Ga t1, Ga t2, Ga z and Ga gust (4). Of these, the three Ga i subtypes function to open atrial potassium channels (6). Ga 16 is a member of the Gq subfamily and is expressed specifically in hematopoietic cells (7).

Supplier: Bioss

Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors (1). Each of a very broad range of receptors specifically detects an extracellular stimulus (a photon, pheromone, odorant, hormone or neurotransmitter) while the effectors (i.e., adenylyl cyclase), which act to generate one or more intracellular messengers, are less numerous. In mammals, G protein alpha, Beta and Gamma polypeptides are encoded by at least 16, 4 and 7 genes, respectively (2-5). Most interest in G proteins has been focused on their a subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Four distinct classes of G alpha subunits have been identified; these include Gs, Gi, Gq and Ga 12/13 (3,4). The Gi class comprises all the known a subunits that are susceptible to pertussis toxin modifications, including Ga i-1, Ga i-2, Ga i-3, Ga o, Ga t1, Ga t2, Ga z and Ga gust (4). Of these, the three Ga i subtypes function to open atrial potassium channels (6). Ga 16 is a member of the Gq subfamily and is expressed specifically in hematopoietic cells (7).

Supplier: Bioss

Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors (1). Each of a very broad range of receptors specifically detects an extracellular stimulus (a photon, pheromone, odorant, hormone or neurotransmitter) while the effectors (i.e., adenylyl cyclase), which act to generate one or more intracellular messengers, are less numerous. In mammals, G protein alpha, Beta and Gamma polypeptides are encoded by at least 16, 4 and 7 genes, respectively (2-5). Most interest in G proteins has been focused on their a subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Four distinct classes of G alpha subunits have been identified; these include Gs, Gi, Gq and Ga 12/13 (3,4). The Gi class comprises all the known a subunits that are susceptible to pertussis toxin modifications, including Ga i-1, Ga i-2, Ga i-3, Ga o, Ga t1, Ga t2, Ga z and Ga gust (4). Of these, the three Ga i subtypes function to open atrial potassium channels (6). Ga 16 is a member of the Gq subfamily and is expressed specifically in hematopoietic cells (7).

Supplier: Bioss

Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors (1). Each of a very broad range of receptors specifically detects an extracellular stimulus (a photon, pheromone, odorant, hormone or neurotransmitter) while the effectors (i.e., adenylyl cyclase), which act to generate one or more intracellular messengers, are less numerous. In mammals, G protein alpha, Beta and Gamma polypeptides are encoded by at least 16, 4 and 7 genes, respectively (2-5). Most interest in G proteins has been focused on their a subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Four distinct classes of G alpha subunits have been identified; these include Gs, Gi, Gq and Ga 12/13 (3,4). The Gi class comprises all the known a subunits that are susceptible to pertussis toxin modifications, including Ga i-1, Ga i-2, Ga i-3, Ga o, Ga t1, Ga t2, Ga z and Ga gust (4). Of these, the three Ga i subtypes function to open atrial potassium channels (6). Ga 16 is a member of the Gq subfamily and is expressed specifically in hematopoietic cells (7).

Supplier: ERLAB CAPTAIRE

The Captair® Smart ductless filtering hoods are designed with a simple and innovative way of communication. Smart Technology, simple and intuitive communication by light indicates the status of the hood. A simple light pattern will indicate if the airflow is compromised, if there is a fan failure or in case of a filter breakthrough.
Please complete the quote request to enable us to assist you in finding the right unit for your application.
https://uk.cmd2.vwr.com/bin/public/fc/main?f=Y5&pimid=16984845

Supplier: Sartorius Balances

These ultra-micro balances from the Cubis® II range are for highly accurate weighing of microgram quantities, there are also models specifically designed for filter weighing applications.

Supplier: Bioss

Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors (1). Each of a very broad range of receptors specifically detects an extracellular stimulus (a photon, pheromone, odorant, hormone or neurotransmitter) while the effectors (i.e., adenylyl cyclase), which act to generate one or more intracellular messengers, are less numerous. In mammals, G protein alpha, Beta and Gamma polypeptides are encoded by at least 16, 4 and 7 genes, respectively (2-5). Most interest in G proteins has been focused on their a subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Four distinct classes of G alpha subunits have been identified; these include Gs, Gi, Gq and Ga 12/13 (3,4). The Gi class comprises all the known a subunits that are susceptible to pertussis toxin modifications, including Ga i-1, Ga i-2, Ga i-3, Ga o, Ga t1, Ga t2, Ga z and Ga gust (4). Of these, the three Ga i subtypes function to open atrial potassium channels (6). Ga 16 is a member of the Gq subfamily and is expressed specifically in hematopoietic cells (7).

Supplier: Merck

TLC plates, unmodified silica gel 60
This range of plates uses Merck silica gel 60 with a unique polymeric binder that results in a uniform, hard surface that will not easily crack or blister. The smooth, extremely dense plate coating ensures narrow bands and maximum separation efficiency with the lowest background noise. Layer thickness 200 μm and, particle size of 10 to 12 µm. Flexible aluminium plates that can easily be cut to match individual separation requirements. Two inorganic fluorescent indicators for UV detection of colourless substances. Samples which absorb shortwave UV at 254 nm are detected due to fluorescence quenching.

Supplier: MP Biomedicals

Cetyltrimethylammonium bromide is a phase transfer catalyst ideal for the precipitation of DNA. Hexadecyltrimethylammonium bromide is a bactericidal, cationic detergent. Its activity is neutralized by soaps and anionic detergents (i.e., SDS). Trimethylammonium bromide compounds form insoluble complexes with SDS. It is active at alkaline pH against both Gram positive and Gram negative organisms.

Cetyltrimethylammonium bromide ia used as an cationic surfactant suitable for the isolation of high molecular weight DNA in plants and other organisms(by a rapid method) as well as plant DNA for use in PCR analysis. It has been used to precipitate nucleic acids. CTAB provides enhancement of Concanavalin A mediated agglutination. It has been used for the determination of protein molecular weights in electrophoretic systems and for determination of critical micelle concentration of detergents. CTAB has also been used as a titrant for potentiometric titration of perchlorate and as a phase-transfer catalyst in reduction of arenes and heterocyclic compounds.

Supplier: Portwest

Contemporary work trouser made from highly durable ripstop fabric that stretches as you move. Subtle articulation on the knees allow for increased freedom of movement and a high rise back waistband ensures protection in all working positions. Packed with functionality including pre-bent knees, a crotch gusset, two tier knee pad pockets and an adjustable leg length for added convenience.

Supplier: HAMILTON BONADUZ

These syringes are the Gastight® version of the original 700 series syringes and are ideal for dispensing volumes from 1 µl up to 500 µl.

Supplier: Biotium

Label your antibody with one of our superior CF® Dyes in 30 minutes without a purification step. Labelling tolerates many common buffer components including BSA and ascites.

Supplier: Ahlstrom-Munksjö

Ahlstrom-Munksjö’s qualitative grades of filter paper are recommended for use in analytical methods which determine or identify particulate constituents of a mixture irrespective of the amount present. Qualitative filter papers are often used in routine separation work that still requires high purity and consistent performance. These filter papers covers a wide range of laboratory applications, as liquids clarification, qualitative analytical separations for precipitates and buffers filtration, and are also used for soil analysis and in food and beverage testing. Wet-strengthened grades contain a reinforcement agent that increases their resistance to rupture when wet, such as in vacuum filtration, and their resistance when filtering acidic solutions.

Supplier: SGI - DNA Synthetic Genomics

The Gibson Assembly® HiFi 1 Step Kit provides a simple and effective method to seamlessly construct synthetic genes, genetic pathways, as well as entire genomes. This method allows researchers to achieve complex assembly of large DNA constructs, with multiple inserts, in 1 hour.

Supplier: Boster Bio

Rabbit IgG polyclonal antibody for Plectin(PLEC) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Supplier: ProSci Inc.

There are two types of cytokeratins/keratins/CKs: the acidic type I cytokeratins and the basic or neutral type II cytokeratins. The subsets of cytokeratins which an epithelial cell expresses depends mainly on the type of epithelium, the moment in the course of terminal differentiation and the stage of development. Thus this specific keratin fingerprint allows the classification of all epithelia upon their keratin expression profile. Furthermore this applies also to the malignant counterparts of the epithelia (carcinomas), as the keratin profile tends to remain constant when an epithelium undergoes malignant transformation. The main clinical implication is that the study of the keratin profile by immunohistochemistry techniques is a tool of immense value widely used for tumor diagnosis and characterization in surgical pathology. [Wiki].

Supplier: Cytiva

HiTrap IMAC FF is prepacked with IMAC Sepharose 6 Fast Flow. The resin is charged with the metalion of your choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent purification of polyhistidine tagged proteins.