51326 Results for: "Cover+Glasses"

Supplier: MP Biomedicals

Storage: Room Temperature, desiccate, store under nitrogen.
Dimethyl Sulfoxide is a dipola, aprotic solvent. It has been shown to accelerate strand renaturation (1-10% concentration) and is believed to give the nucleic acid thermal stability against depurination.
Dimethyl Sulfoxide is used to enhance dermal absorption of many chemicals, as a solvent for many organic and inorganic compounds including fats, carbohydrates, dyes, resins, and polymers, in antifreeze or hydraulic fluids, as a cryopreservative for cell cultures, oxidation of thiols and disulfides to sulfonic acids, as a PCR cosolvent to help improve yields, especially in long PCR. DMSO is routinely used in polymerase chan reaction (PCR), amplification of cDNA libraries, DNA sequencing, column-loading buffers for poly (A)+ RNA selection, buffers for the transformation of competent E. coli, and transfection protocols.
To prepare sterile solutions use a teflon or nylon membrane to sterile-filter the DMSO - do not use a cellulose acetate membrane.

Supplier: MP Biomedicals

Storage: +4°C, protect from light
3- (4,5-Dimethyl-2-thiazolyl-2)-2,5-diphenyl-tetrazolium bromide is used as a colorimetric metabolic activity indicator in cell viability assays. Thiazolyl Blue Tetrazolium Blue (MTT) is a dye used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.

Supplier: MP Biomedicals

Storage: Room Temperature, desiccate, store under nitrogen.
Ferrous sulfate heptahydrate is a reagent used in the manufacturing of iron compounds, in electroplating baths, aluminium etching, process engraving and lithography, and in redox polymerisation. It is used as a reducing agent in chemical processes, leather dyes and writing inks. Also, it is a component of weed killers, wood preservatives and water treatment processes. Used In prevention of chlorosis in plants and therapeutically as hematinic. In veterinary medicine, it is used as an astringent to combat iron deficiency. Ferrous sulfate heptahydrate is used in the determination of phosphorus as per the method of Taussky and Shorr. Ferrous sulfate heptahydrate is used in cell culture applications generally bound to transferrin, citrate or other iron transport molecules.

Supplier: BioAir S.p.A.

S@femate TOTAL Cabinets have been designed according to the airflow specifications for NSF49 Class II Type B2 cabinets, also known as 'total exhaust'. 100% fresh air is taken in from the lab, either from the front aperture and from a main inlet. The air from the main inlet is then cleaned by the main HEPA H14 filter to provide an ISO5 working area and joins with the air from the front aperture on the bottom of the cabinet. All the air is then circulated behind the backwall of the unit and filtered via the exhaust HEPA H14 filter and reinjected into the environment.

Supplier: BioTool

The LabFlame+  is optimised for use in microbiology laboratories. Its stainless steel housing is made of one piece, free of any gaps or rims and therefore easy to clean and fully UV resistant. Its optimised air-flow system allows a uniform and constant flame. Connectable to any kind of gas sources. Ideal for use in cleanroom workbenches and laboratories.

Supplier: Honeywell Chemicals

1,10-Phenanthroline hydrochloride monohydrate 99.5-102% (dried basis) Reag. Ph. Eur. for spectrophotometry, Fluka™

Supplier: Binder

A powerful all-rounder among constant climate chambers: Equipped with all kinds of sophisticated technology, the KBF PRO with proven APT.line™ preheating chamber technology takes any material test in its stride – whether it takes place at -20 °C or under THB conditions at +85 °C and 85% relative humidity. Units have a stainless-steel inner chamber and temperature range of -20 to +100 °C and humidity range of 10% to 98% RH. The models offer flexible water supply options for all installation sites and consumes up to 40% less energy than its predecessor, the KMF model. Optional light modules are also available to turn the KBF PRO into a chamber for ICH photostability testing or plant growth.

Supplier: LABCONCO

Self-contained ductless enclosures allow safe handling of organic, formaldehyde, acid gas, sulphur compounds, or ammonia chemical fumes and vapours, as well as particulates. Air inside the enclosures passes through carbon filters, and in some cases additional HEPA particulate filters, before being safely exhausted back into the laboratory. No outside ducting is required.

Supplier: Thermo Fisher Scientific

Thermo Scientific Pierce TCEP-HCl is a potent, versatile, odorless, thiol-free reducing agent with broad application to protein and other research involving reduction of disulfide bonds. The unique compound is easily soluble and very stable in many aqueous solutions. TCEP reduces disulfide bonds as effectively as dithiothreitol (DTT), but unlike DTT and other thiol-containing reducing agents, TCEP does not have to be removed before certain sulfhydryl-reactive crosslinking reactions.

Supplier: Heidolph Instruments GmbH & Co.KG

Hei-VAP Industrial large scale rotary evaporators for enhanced vacuum control standards and for maximum safety.

Supplier: MP Biomedicals

Eosin Y disodium salt is used in combination with Azure II to show morphological details of marrow cells; buffered Azure A/Eosin Y method for demostrating cell granules, microorganisms and nuclei; for showing insect musculature; for demonstrating cells infected with mosquito-iridescent virus; use in combination with Alum Hematoxylin/Azure II to show mast cells, early necrobiotic changes in cells and protozoans, rickettsias and bacteria in tissues; to demonstrate volutin granules in any microorganism; Bond's modification of Mann's Eosin-Methyl Blue for Negri bodies in smears; Harris stain for Negri bodies in smears; Mann's method for staining Negri bodies in sections; Gridley's stain for Endamoeba in tissue sections; a modified methenamine-silver nitrate method for the demonstration of Endamoeba in sections. It is used as a counterstain for hematoxylin.

Supplier: VWR Collection

User friendly instrument, with articulated electrode stand and IP 43-rated housing, provides high resolution and accuracy for precise multi-parameter measurements. This meter can either measure pH/mV and conductivity or pH/mV and oxygen simultaneously. Complete with USB output for connection to a computer. GLP compliant.

Supplier: LABCONCO

Clean benches provide product protection from environmental contaminants for applications requiring a particulate-free work area, including plant tissue culture, electronic part inspection, syringe filling, medical device assembly, media plate preparation, and PCR.

Supplier: Molekula

L-Arginine hydrochloride

Supplier: Lovibond Tintometer

A series of spectrophotometric colorimeters for colour grading of optically clear samples. Each version of the PFXi 195 includes a selection of standard colour scales that is used in a specific industry sector. Additional scales can be added, at the time of order or remotely, as optional upgrades, to provide the flexibility to meet individual requirements. Results can also be displayed in terms of spectral data and CIE values. For product types that are incompatible with the standard colour scales, the PFXi 195 software allows the users to build up a customised scale from a series of reference samples, and then to obtain the closest match to the stored reference.

Supplier: Thermo Orion

Use the Orion Star T920 redox titrator for dedicated redox titrations including sulphite/SO₂ and reducing sugar in juice and wine, ascorbic acid (vitamin C) and peroxide value in food products, dissolved oxygen in wastewater by Winkler titration and organic matter in soil.

Supplier: MP Biomedicals

p nitrophenol is the substrate of choice for use with alkaline phosphatase in ELISA procedures. This substrate produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 M NaOH and read at 405 nm.

Supplier: Azbil Telstar Technologies

The ergonomically designed BiOptima Class II microbiological safety cabinet ensures optimum efficiency and high-level energy savings. BiOptima provides operator protection with the inflow air barrier, product protection thanks to HEPA-filtered laminar downflow in the working area and environmental protection through the exhaust HEPA filter.

Supplier: MP Biomedicals

Storage: +4 °C
Pyruvic acid is an intermediate in sugar metabolism and in enzymatic carbohydrate degradation (alcoholic fermentation) where it is converted to acetaldehyde and CO2 by carboxylase. In muscle, pyruvic acid (derived from glycogen) is reduced to lactic acid during exertion, which is reoxidized and partially retransformed to glycogen during rest. It improves coliform recovery when present in culture medium. It is involved in a metabolic regulatory pathway activated by mitochondrial oxidants. Pyruvate is involved in respiratory regulation in plants by interacting with alternative oxidase at a conserved cysteine residue. It may help prevent hydrogen peroxide mediated cell death.
Sodium pyruvate is utilized as a component in culture broth and media. Sodium pyruvate is used in Wallen fermentation medium to enhance the conversion of oleic acid to 10-ketostearic acid by Bacillus sphaericus. Sodium pyruvate has also been used to establish stably transfected human B cell lines.
Sodium Pyruvate has shown antioxidant properties and protective effects against oxygen radicals. Pyruvate is produced as part of glycolysis and is an intermediate in many metabolic pathways. It can be converted into acetyl CoA and enter the TCA Cycle.

Supplier: VWR Collection

The ILCO 180 and ILCO 260 Premium offer optimal growth conditions for cell cultures and meet demanding requirements for best possible cell growth and maximum sample safety. These CO₂ incubators come with a built-in, drift-free infrared CO₂ sensor that can be sterilised. Reliable decontamination is provided by the standard-compliant 180 °C hot air sterilisation routine which completely eliminates contaminants and ensures the inner chamber is completely sterilised. They have an intuitive menu and user friendly software, also USB port for data download to USB stick and for uploading programs. They can be connected to Ethernet or Wi-Fi networks for remote control from any computer, and users can receive notifications about alarms/events via e-mail.

Supplier: MP Biomedicals

Tweens® are a series of nonionic surfactants derived from sorbitan esters. They are soluble or dispersible in water but differ widely in organic and oil solubilities. Used as oil-in-water emulsifiers in pharmaceuticals, cosmetics, cleaning compounds, etc.
Tween® 80 has been widely used in biochemical applications including: solubilizing proteins, isolating nuclei from cells in culture selective protein extraction growing of tubercule bacilli, and emulsifying and dispersing substances in medicinal and food products. It has little or no activity as an anti-bacterial agent. It has been shown to have an adverse effect on the antibacterial effect of methyl paraben and related compounds. Non-ionic detergent used for selective protein extraction and isolation of nuclei from mammalian cell lines.
Soluble/miscible in water to give a clear yellow solution; miscible with alcohol, dioxane, and ethyl acetate; practically insoluble in liquid paraffin and fixed oils (such as mineral oil).
Autoclaving of solutions is not recommended. Sterile filtering is suggested with a 0.22 micron filter. Tween® may need to be warmed to about 40 °C and alternated with portions of hot distilled water while being poured through the filter.
Store at Room Temperature (15-30 °C).

Supplier: MP Biomedicals

Storage: +4 °C
D-Biotin is a growth factor present in small amounts in every living cell. It is involved in naturally occurring carboxylation reactions. It occurs mainly bound to proteins or polypeptides. It is more abundant in the liver, kidney, pancreas, yeast and milk. Biotin levels are higher in cancerous tumors than in normal tissues. It is inactivated by binding to avidin.
D-Biotin may be used to elute proteins from avidin/streptavidin resins. It is widely used for dietary supplements and fortified foods. It is also used for tablets and hard-shell capsule preparation due to its pharmaceutical properties.
Essential vitamin that is important for amino acid and energy metabolism, and fatty acid synthesis. It is a prosthetic group in four mammalian carboxylase families and facilitates the binding and transfer of carbon dioxide.
Soluble in water (22 mg/100 mL), ethanol (80 mg/100 mL), more soluble in hot water and in dilute alkalies; insoluble in other common organic solvents. Soluble in 2 M Ammonium hydroxide (50 mg/mL - clear, colorless solution), dimethylformamide (1.7 mg/mL).

Supplier: Lovibond Tintometer

Two spectrophotometric colorimeters that each include several of the popular scales that meet the requirements of objective colour analysis of oils, chemicals, fuels and other transparent products. The PFXi 950 instruments are rugged colorimeters with fabricated steel housings, which function equally as QC instruments within the laboratory or on 24-hour operation in a production environment. The accuracy and repeatability of the colour data provided by these colorimeters ensures consistency in colour measurement, giving processors the confidence needed to make important decisions regarding raw materials and refining operations.

Supplier: VWR Collection

These compressor-cooled incubators provide a temperature range between –10 to +100 °C with forced air convection and fan speed control (10 to 100%). Featuring a stainless steel (DIN 1.4301) inner chamber and a powder coated sheet exterior. PREMIUM models have an intuitive menu and user friendly software, USB port with flash drive for data download to USB stick and for uploading programs. They can be connected to Ethernet or Wi-Fi network for remote control from any computer and users can receive notifications about selectable parameters via e-mail.

Supplier: Thermo Fisher Scientific

Heratherm™ large capacity incubators were designed to prevent samples drying out. All models have a large, easy to view, vacuum fluorescence display and simple to use touch button operation which is controlled by a microprocessor and automatic over-temperature alarm system to protect samples.

Supplier: MP Biomedicals

Storage: Store at -20 °C. Store Desiccated. Store Under Nitrogen. Protect from light.
5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, commonly known as X−Gal, is a histochemical substrate for β−galactosidase.
5-Bromo-4-Chloro-3-Indolyl-β-D-Galactopyranoside is used as indigogenic substrate for β-galactosidase, for detection of β-galactosidase-positive clones, and the identification of lac and bacterial colonies or phage plaques. It is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype. X−Gal is cleaved by β−galactosidase to yield an insoluble blue precipitate. X−Gal is particularly useful in molecular biology applications to detect the activity of β−galactosidase which is frequently used as a reporter gene. In cloning, X−Gal is used to detect insertion of foreign DNA into the lacZ region of plasmid DNA using α-complementation which is based on vectors such as the pUC and the M13mp series that carry a fragment of the β-galactosidase gene encoding an α-fragment of β-galactosidase.

Supplier: MP Biomedicals

Storage: Store at Room Temperature (15-30 °C).
D-myo-Inositol is a component of membrane phospholipids, glycosylphophatidylinositol anchors that bind glycoproteins to cell membranes, and inositol phosphate second messengers. It is a growth factor for animals as well as microorganisms. Myo-Inositol is the most abundant form of polyols that serves as a structural element of secondary messengers in eukaryotic cells. Inositol or cyclohexane-1,2,3,4,5,6-hexol is a chemical compound, a sixfold alcohol (polyol) of cyclohexane. It exists in nine possible stereoisomers, of which the most prominent form, widely occurring in nature, is cis-1,2,3,5-trans-4,6-cyclohexanehexol, or myo-inositol (former name meso-inositol). Inositol is a carbohydrate, though not a classical sugar. It has a taste which has been assayed at half the sweetness of table sugar. Used as a lipotropic agent.
A component of membrane phospholipids, glycosyl­phosphatidyl­inositol anchors that bind glycoproteins to cell membranes, and inositol phosphate second messengers. In addition, inositol serves as an important component of the structural lipids phosphatidylinositol (PI) and its various phosphates, the phosphatidylinositol phosphate (PIP) lipids.

Supplier: MP Biomedicals

Storage: Store at Room Temperature (15-30 °C).
Ethylenediamine Tetraacetic Acid is a polyamino carboxylic acid hexadentate ligand and a chelating agent. This product is designated as Molecular Biology grade and is suitable for molecular biology applications.
Ethylenediamine Tetraacetic Acid is a chelator of divalent cations. It also inhibits enzymes, such as metalloproteases, that require divalent cations for activity.

Supplier: Thermo Fisher Scientific

D-(+)-Camphor ≥98%

Supplier: MP Biomedicals

Storage: Store at -20 °C.
Brefeldin A is a fungal metabolite which is a macrocyclic lactone exhibiting a wide range of antibiotic activity. Produced by Penicillium brefeldianum. Blocks binding of the cytosolic coat protein b-COP and ARF to Golgi membranes mediated by protein G. Also blocks protein transportation into post-Golgi compartments. It activates the sphingomyelin cycle. Brefeldin A mediated apoptosis has been observed in human tumor cells.
Brefeldin A reversibly inhibits the intracellular translocation of proteins in eukaryotes, e.g., during transport of proteins to the cell surface for secretion or expression. It has been reported to block the response of cultured cells to cholera toxin. In HepG2 cells, BFA induces two blocks in the secretory pathway; one at the level of the endoplasmic reticulum-Golgi juncture and the other in the trans-Golgi network. Brefeldin A is used in the studies of Brefeldin A-inhibited Guanine Nucleotide-exchange Protein, BIG2, Regulates the Constitutive Release of TNFR1 Exosome-like Vesicles.
Brefeldin A (BFA) is a fungal metabolite which disrupts the structure and function of the Golgi apparatus. BFA is an activator of the sphingomyelin cycle. Brefeldin A-mediated apoptosis has been observed in human tumor cells.