140473 Results for: "Chromatography+Learning+Activities"

Supplier: EDVOTEK

Drinking water is routinely tested for contamination. If a screening tests positive, more sophisticated tests are required. One such test uses PCR in multiplex format. In this experiment, students test for the presence of three separate, classroom-safe organisms in a water sample using a single PCR reaction.

Supplier: EDVOTEK

Genetic modification of plants is a controversial area of biotechnology. All such experiments in plants begin with establishing plant cells in culture. This involves de-differentiating plant cells to form plant 'stem cells'. In this experiment, students establish cell cultures of African violets from leaves. They then use plant growth regulators to encourage root growth from the cultured cells, and produce a mature plant.

Supplier: Whatman products (Cytiva)

Cellulose Chromatography and Blotting Paper Roll contains cotton linter filter sheets for electrophoresis and amino acid chromatography.

Supplier: G-Biosciences

A major challenge for molecular biologists and genetic engineers is to easily detect and analyze genetic mutations that occur naturally, causing diseases, or during genetic engineering or cloning, whether deliberate or accidental. Students learn about different types of genetic mutations, including substitutions, deletions and point mutations and about various techniques used to detect genetic mutations. The kit contains all the reagents to screen simulated clinical samples for a mutant gene, using both the polymerase chain reaction (PCR) and restriction digestion mapping. Students conduct a simple clinical diagnostic experiment in order to identify a diseased patient.

Supplier: Avantor

These are used in low pressure preparative chromatography. The mesh specifications ensure there is no excess of fines in the product which can affect flow rates and separation performance.

Supplier: G-Biosciences

The final goal in molecular biology is often the expression of a recombinant protein. The transformed plasmids can be used as templates by the bacteria to produce protein. Students learn about essential promoters and other elements necessary for successful protein expression in bacteria, including the differences between inducible and constitutive (unregulated) expression.The Expression of a Recombinant Protein kit allows students to express a protein either constitutively or under the control of an inducible promoter, which is activated with IPTG (isopropyl-beta-D-thiogalactopyranoside).

Supplier: WATERS

Standards for Ion Pair Chromatography, Paired-ionic chromatography reagent B7, heptane sulfonic acid

Supplier: EDVOTEK

In this experiment, students purify the restriction enzyme, Eco RI. This procedure utilised an ion exchange chromatograph step for Eco RI purification. Column fractions are assayed for the enzyme using Lambda DNA and digestion products are identified by gel electrophoresis. Fractions that contain Eco RI are identified and pooled. The total and specific activities are calculated. Recommended for advanced courses.

Supplier: EDVOTEK

Isolation of high molecular weight chromosomal DNA is the first step in molecular cloning since it is the source of genes in cells. This experiment provides DNA extraction LyphoCells and reagents for isolating chromosomal DNA from E. coli. After spooling from solution, the DNA can be dissolved and analysed by agarose gel electrophoresis as an optional lab extension activity.

Supplier: Avantor

These are disposable extraction columns packed with activated spherical carbon. Designed for the extraction and purification of organic compounds.

Supplier: VARIAN

Chromatography paper is used in thin layer chromatography applications such as evaluating radioisotope purity, QC testing.

Supplier: EDVOTEK

Four experimental modules are combined into one experiment to provide a comprehensive biotechnology exploration focusing on the green fluorescent protein (GFP). Bacterial cells are transformed to express the GFP. The transformed cells are then grown and the GFP purified by column chromatography. Finally, the purity of the protein fractions are analysed by SDS polyacrylamide electrophoresis.

Supplier: EDVOTEK

The second assay used to confirm a positive HIV ELISA result is the Western blot. Using this kit, students separate protein samples from hypothetical patients on agarose gels. The proteins are then transferred to a membrane and simulated HIV proteins are detected.

Supplier: 3B Scientific

Evacuated glass flask containing i.a. electron gun with directly heated tungsten wire cathode and a cylindrical, collimating anode. The generated beam is allowed to stroke along a semi-transparent mica disc in the center of the tube. Luminescent coating on the front of the disc makes the beam appear. Two of the edges of the back are provided with graduated scales.

Supplier: EDVOTEK

Educational Materials, Microbiology, Application: Microbiology, ReadyPour™ LB agar with ampicillin

Supplier: DIDALAB

These wide diameter lenses are used for easy classroom experiments and for aberrations underscoring.

Supplier: EDVOTEK

Kit for teaching students how to extract and precipitate their own DNA. Students can transfer their DNA to a tube that can be used as a pendant on a necklace.

Supplier: EDVOTEK

The mitochondria are thought to have evolved from a symbiotic relationship between prokaryotic and eukaryotic cells. Mitochondria have their own DNA and are only inherited via the maternal line. In this experiment, students amplify two regions of their mitochondrial DNA.

Supplier: EDVOTEK

This experiment introduces a simple method to determine protein subunit molecular weights using horizontal electrophoresis. As the protein standards and 'unknowns' are prestained, the separation of proteins can be observed during electrophoresis. Included in the experiment is protein-grade agarose, which provides an alternative to the use of polyacrylamide gels.

Supplier: EDVOTEK

Southern blotting is an important technique used widely in clinical genetics and research. By transferring DNA from an agarose gel onto a membrane the method allows the precise analysis and identification of DNA bands on a gel. Using this kit, students use Southern blotting to find a point mutation in the haemoglobin gene indicating Sickle Cell Anaemia.

Supplier: G-Biosciences

The kit utilises the principle of diffusion of nucleic acids on a nylon membrane to determine their concentration. No spectrophotometers required.

Supplier: EDVOTEK

Bovine spongiform encephalophathy (BSE), also known as Mad Cow Disease, is a neurodegenerative, fatal condition in cattle. Consuming BSE-infected beef is believed to be the cause of a similar condition in humans, Creutzfeldt-Jakob disease. In this experiment, students examine simulated PCR products from several feed mills, to determine any possible violations of the ban on including animal parts in cattle feed.

Supplier: 3B Scientific

Educational Materials, Electricity/Magnetism, Application: Physics, Analogue oscilloscope, 30 MHz, microprocessor controlled

Supplier: 3B Scientific

Vane wheel device is used for demonstrating air and heat flows generated by a burning candle, water vapour or other heat sources. Made of Aluminium and mountable on a long needle.

Supplier: EDVOTEK

Microarray technology allows scientists to screen large numbers of samples in one assay. This technology allows high-throughput screening of small samples and allows comparisons to be made between healthy and unhealthy tissues. In this experiment, students apply simulated DNA and RNA samples to a membrane to screen for positive and negative samples.

Supplier: 3B Scientific

Evacuated glass flask containing i.a. electron gun with directly heated tungsten wire cathode and a cylindrical anode. The electron gun emits a divergent electron beam, aimed at an Maltese cross of aluminum placed in the middle of the tube. The cross can be connected electrically. At the same time, light from the filament is also directed towards the cross, so that a comparison can be made between the shadows of different types that appear on the luminescent surface at the far end of the tube. Specifications.

Supplier: 3B Scientific

Educational Materials, Electricity/Magnetism, Application: Physics, Adapter, BNC connector to 4 mm Jack plugs with 19 mm spacing.

Supplier: DIDALAB

A range of digital storage oscilloscopes with bandwidth from 150 to 500 MHz.

Supplier: EDVOTEK

Infectious agents such as bacteria and viruses can spread rapidly through a population and cause widespready disease and death. In this experiment, students use coloured solutions to simulate the spreading of a disease in the classroom.

Supplier: 3B Scientific

Highly evacuated electron tube for determining the wavelength of electrons as a function of the anode voltage from the radii of the diffraction rings and the distance between the lattice planes of graphite (Debye-Scherrer-diffraction) and rendered visible on a fluorescent screen. Also intended for determining the wavelengths of monochromatic radiation at different anode voltages from the radii of the diffraction rings and the lattice plane spacing of graphite, as well as confirming de Broglie’s hypothesis.