140473 Results for: "Chromatography+Learning+Activities"

Supplier: EDVOTEK

This kit is for teaching students how people's physical traits are a reflection of their genes. In this simulation, students use electrophoresis to separate dyes which represent genetic traits.

Supplier: G-Biosciences

The final goal in molecular biology is often the expression of a recombinant protein. The transformed plasmids can be used as templates by the bacteria to produce protein. Students learn about essential promoters and other elements necessary for successful protein expression in bacteria, including the differences between inducible and constitutive (unregulated) expression.The Expression of a Recombinant Protein kit allows students to express a protein either constitutively or under the control of an inducible promoter, which is activated with IPTG (isopropyl-beta-D-thiogalactopyranoside).

Supplier: G-Biosciences

A major challenge for molecular biologists and genetic engineers is to easily detect and analyze genetic mutations that occur naturally, causing diseases, or during genetic engineering or cloning, whether deliberate or accidental. Students learn about different types of genetic mutations, including substitutions, deletions and point mutations and about various techniques used to detect genetic mutations. The kit contains all the reagents to screen simulated clinical samples for a mutant gene, using both the polymerase chain reaction (PCR) and restriction digestion mapping. Students conduct a simple clinical diagnostic experiment in order to identify a diseased patient.

Supplier: EDVOTEK

In this experiment, students study the diversity of fish using pre-stained, lyophilised proteins. Total protein from perch, walleye and salmon is extracted and pre-stained using an indicator dye. Each fish protein sample has a characteristic banding pattern when separated by denaturing SDS-polyacrylamide gel electrophoresis, which can be used to identify the specific species.

Supplier: EDVOTEK

Coronary heart disease and stroke are major causes of death in the Western world. Elevated blood cholesterol levels are a serious risk factor in both conditions. The genetic disease familial hypersholesterolemia (FH) causes an increase in blood levels of the bad form of cholesterol, low density lipoprotein (LDL). In untreated patients with the mutant FH gene, the condition can cause premature death. This experiment introduces the colorimetric enzymatic reaction which is the basis of the clinical cholesterol test. In addition, using agarose gel electrophoresis, students can analyse a simulated genetic screening for a disease.

Supplier: G-Biosciences

Antibiotics play a crucial role in the manipulation, screening and killing of bacteria in a range of biotechnology processes. This kit specifically teaches the basic principles of antibiotics, bacterial resistance and susceptibility. Students learn and understand the use of antibiotic resistance in screening for infectious diseases. Utilising a bacterial strain, students learn the effects of different antibiotics and visualise bacterial sensitivity and resistance to the supplied antibiotics. This method involves the use of filter paper discs impregnated with a specified concentration of antibiotics on the surface of an agar plate containing microbial cells. This kit will enable students to analyse the inhibitory effects of different antibiotics on selected bacterial cells and then determine which antibiotic is the most suitable to treat a bacterial infection.

Supplier: DIDALAB

Economical, recent design of prismatic optical bench

Supplier: Thermo Fisher Scientific

Replace metal-type troughs with the polypropylene pneumatic trough.

Supplier: GLASWARENFABRIK KARL HECHT

According to Hoffmann, with two city dishes, 0 to 30 ml of grading graded 1/10 ml.

Supplier: DIDALAB

Extremely luminous beam, ideal for classroom experiments. Class IIIa (5 mW Power)

Supplier: EDVOTEK

Ready-to-Load™ kit for introducing the use of restriction enzymes as a tool to digest lambda DNA at specific nucleotide sequences.

Supplier: EDVOTEK

Ready-to-Load™ PCR kit introduces students to the principles and applications of the Polymerase Chain Reaction (PCR). This simulation experiment does not contain human DNA and does not require a thermal cycler.

Supplier: EDVOTEK

Students use primers for a 300 base pair Alu insertion in chromosome 16 (PV92) to determine their own genotype. They can then compare their class results with others around the world over the internet.

Supplier: 3B Scientific

Digital counter/timer for measuring duration of motion, transition times, periods, pendulum periods, frequencies, as well as for counting events or Geiger tube pulses. Includes a speaker that can be turned on and off and power supplies for direct connection to photo gate or a Geiger-Müller counter.

Supplier: EDVOTEK

Using prestained LyphoProteins, subunit molecular weights are determined by analysis using denaturing SDS vertical polyacrylamide gel electrophoresis. Prestained proteins with unknown molecular weights are assigned molecular weights based on the relative mobility of prestained standard protein markers.

Supplier: EDVOTEK

In this experiment, transformed cells take up a plasmid containing the Green Fluorescent Protein (GFP) gene. The GFP gene was isolated from the jellyfish Aequorea victoria. Transformed colonies expressing the GFP protein are visibly green in normal light but will fluoresce brightly when exposed to longwave UV light.

Supplier: EDVOTEK

Suppressor genes, such as p53, are essential for cell functions. Mutations in the p53 gene can be correlated to predisposition for certain cancers. Mutations of genes can either be inherited or accumulated due to environmental insults. This experiment deals with a family pedigree determination of several generations relating to cancer formation due to p53 gene mutation.

Supplier: EDVOTEK

Drinking water is routinely tested for contamination. If a screening tests positive, more sophisticated tests are required. One such test uses PCR in multiplex format. In this experiment, students test for the presence of three separate, classroom-safe organisms in a water sample using a single PCR reaction.

Supplier: Avantor

The J.T.Baker® BAKERBOND® Poly Hi-Propyl™ mixed mode hydrophobic interaction chromatography (HIC) resin provides improved selectivity for superior separation in process chromatography applications.

Supplier: EDVOTEK

Genetic modification of plants is a controversial area of biotechnology. All such experiments in plants begin with establishing plant cells in culture. This involves de-differentiating plant cells to form plant 'stem cells'. In this experiment, students establish cell cultures of African violets from leaves. They then use plant growth regulators to encourage root growth from the cultured cells, and produce a mature plant.

Supplier: DIDALAB

Triangular, cast aluminium optical bench

Supplier: NEUBERT VOLUME GLASSWAERE

Electrolysis apparatus according to Hoffmann, made of borosilicate glass 3.3.

Supplier: Whatman products (Cytiva)

Cellulose Chromatography and Blotting Paper Roll contains cotton linter filter sheets for electrophoresis and amino acid chromatography.

Supplier: DIDALAB

Optical glass components are manufactured with high accuracy polishing and flatness tolerance.

Supplier: EDVOTEK

This experiment presents a bioterrorism scenario, with students examining a simulated DNA fingerprinting test for the detection of smallpox.

Supplier: EDVOTEK

In this experiment, students purify the restriction enzyme, Eco RI. This procedure utilised an ion exchange chromatograph step for Eco RI purification. Column fractions are assayed for the enzyme using Lambda DNA and digestion products are identified by gel electrophoresis. Fractions that contain Eco RI are identified and pooled. The total and specific activities are calculated. Recommended for advanced courses.

Supplier: EDVOTEK

In this experiment, students see a blue colour change in transformed cells due to the switching on of a gene. The pGAL plasmid gives a blue colour dye due to the production of the β-galactosidase protein by the lacZ gene. IPTG is not required in this experiment since pGAL contains the complete lacZ gene.

Supplier: EDVOTEK

Isolation of high molecular weight chromosomal DNA is the first step in molecular cloning since it is the source of genes in cells. This experiment provides DNA extraction LyphoCells and reagents for isolating chromosomal DNA from E. coli. After spooling from solution, the DNA can be dissolved and analysed by agarose gel electrophoresis as an optional lab extension activity.

Supplier: WATERS

Standards for Ion Pair Chromatography, Paired-ionic chromatography reagent B7, heptane sulfonic acid

Supplier: EDVOTEK

Four experimental modules are combined into one experiment to provide a comprehensive biotechnology exploration focusing on the green fluorescent protein (GFP). Bacterial cells are transformed to express the GFP. The transformed cells are then grown and the GFP purified by column chromatography. Finally, the purity of the protein fractions are analysed by SDS polyacrylamide electrophoresis.