140473 Results for: "Chromatography+Learning+Activities"

Supplier: EDVOTEK

Learn the basics of DNA sequencing and sequence homology with this lab activity.

Supplier: Honeywell Chemicals

Aluminium oxide activated basic Brockmann Grade I ~150 mesh, Fluka™

Supplier: Honeywell Chemicals

Aluminium oxide activated neutral Brockmann Grade I ~150 mesh, Fluka™

Supplier: Honeywell Chemicals

Aluminium oxide activated acidic Brockmann Grade I ~150 mesh, Fluka™

Supplier: BUCHI

PURE chromatography systems for flash applications.

Supplier: G-Biosciences

Dialysis is a routinely used technique in research laboratories to 'change' the solution a biomolecule is dissolved in. Often the buffers used to isolate biomolecules, such as proteins, are not compatible with downstream applications due to high concentrations of reagents, such as salts. A sample is placed in a bag with a semi-permeable membrane and then placed in the new solution or water. Small molecules, such as salt, can pass through the membrane, moving from an area of high concentration to low concentration. The larger molecules, such as proteins, are retained in the bag. Microdialysis is a modification of dialysis to overcome the problem of dialysing small volumes of precious samples. Microdialysis uses small devices designed for small volumes. This kit allows students to study dialysis using patented microdialysis devices.

Supplier: BUCHI

C-900 PURE Essential chromatography pump system with flexible software control.

Supplier: G-Biosciences

The physical properties of proteins kit is a lab activity that enables students to investigate the physical properties of several different proteins. Students will learn about protein solubility and how it is affected by various parameters; including temperature, pH, salt and dielectric constant. They will understand about protein precipitation due to pH, high salt and in the presence of organic solvents and about protein denaturation as a result of high temperature. In addition, the kit will demonstrate how non-protein agents, such as detergents drastically alter the physical properties of protein molecules and as a result, understand the importance of detergents in protein solubilisation. This lab activity involves analysis of three different types of pure proteins and then students alter some of those properties with a detergent and re-examine physical properties of those proteins. Students are challenged to consider how physical properties of protein molecules can be exploited for purification and characterisation of proteins and apply their findings on a test sample of complex tissue extract.

Supplier: EDVOTEK

This easy and safe experiment allows students to learn about enzyme catalysis, the nature of enzyme action, and protein structure-function relationship. Students will perform an enzyme assay and determine the rate of the enzymatic reaction.

Supplier: BUCHI

The Pure essential flash chromatography system focuses on the essentials for any basic flash application. The design of the instrument, its modularity, and user-friendliness make it easy for you to take the first steps into automation and profit from increased speed and endless possibilities.

Supplier: Thermo Fisher Scientific

Aluminium oxide activated basic ≥99.9995% Brockmann Grade I 58 angstroms

Supplier: EDVOTEK

For learning about the diversity of proteins by studying the electrophoretic profiles of various sources. Students separate proteins from bacterial, plant, serum, and milk proteins alongside a standard protein marker.

Supplier: ENZO LIFE SCIENCES

Caspase-3 activator

Supplier: EDVOTEK

Genetic testing can be used to identify people with a genetic condition which caused them to have an elevated level of cholesterol and which can be fatal. Students can see how genetic testing is carried out and learn about DNA electrophoresis.

Supplier: EDVOTEK

Solve the mystery of two boys separated from their parents a decade ago. Their biological mother is identified by mitochondrial DNA and their biological father from chromosomal DNA.

Supplier: EDVOTEK

Plasmid and lambda DNA are pre-digested with restriction endonucleases that recognise and cut double-stranded DNA within or near defined base sequences. Digests are separated by agarose gel electrophoresis.

Supplier: 3B Scientific

Track with two trolleys and other accessories for investigating linear motion. The trolleys move with minimum friction on wheels with high-quality ball-bearings. They are fitted with magnets at their front ends for experiments involving both elastic and inelastic collisions.

Supplier: EDVOTEK

Small-scale, rapid isolation of plasmid DNA is a routine procedure used for screening and analysis of recombinant DNAs in cloning and sub-cloning experiments. In this experiment, students isolate plasmid DNA without the use of toxic chemicals such as phenol or chloroform.

Supplier: EDVOTEK

The DNA from bacteriophage Lambda is a well-characterised linear molecule containing six recognition sites for Eco RI (5 distinct sites, 2 are very close in size). In this experiment, Lambda DNA is digested by the Eco RI endonuclease. The digestion products are analysed by agarose gel electrophoresis.

Supplier: G-Biosciences

Following restriction enzyme digestion of DNA molecules, researchers need to rejoin the ends of the DNA to generate recombinant DNA, a process known as ligation. Ligation of DNA is achieved with the bacterial enzyme T4 DNA ligase, which catalyses the formation of phosphodiester bonds. The DNA Ligation kit teaches students about ligation as they ligate several DNA fragments together to make larger pieces of DNA that are easily identified by agarose electrophoresis.

Supplier: G-Biosciences

In this lab activity, students learn to perform simple protein isolation procedures to isolate the protein fingerprint from various fresh tissues. They will compare the protein fingerprints of four different tissues to understand that the function of a particular organ is due to the proteins that are localised to the specific organ. Also included in this kit are four dried protein samples (from mouse liver, brain, heart and lungs) to compare as a control, if fresh tissues cannot be obtained.

Supplier: G-Biosciences

In this lab activity, students learn to perform carefully controlled experiments to generate protein fragments using a proteolysis enzyme and then analyse the protein fragments by electrophoresis. By analysis of protein fragmentation patterns, i.e. protein fingerprints, students learn about protein sequence, structure, and their conservation. Students resolve a set of three functionally identical protein samples selected from throughout the animal kingdom; including human, bovine and sheep. After generating fingerprints by electrophoresis, students examine the protein fingerprint of each sample to determine the degree of conservation.

Supplier: G-Biosciences

Antibiotics play a crucial role in the manipulation, screening and killing of bacteria in a range of biotechnology processes. This kit specifically teaches the basic principles of antibiotics, bacterial resistance and susceptibility. Students learn and understand the use of antibiotic resistance in screening for infectious diseases. Utilising a bacterial strain, students learn the effects of different antibiotics and visualise bacterial sensitivity and resistance to the supplied antibiotics. This method involves the use of filter paper discs impregnated with a specified concentration of antibiotics on the surface of an agar plate containing microbial cells. This kit will enable students to analyse the inhibitory effects of different antibiotics on selected bacterial cells and then determine which antibiotic is the most suitable to treat a bacterial infection.

Supplier: G-Biosciences

This lab activity has two objectives - to deepen the understanding of protein molecules and for students to learn the potential of electrophoresis in protein analysis. Students study the fundamentals of protein structure from their primary structure to the more complex tertiary and quaternary structures, utilising protein electrophoresis. Complex mixtures of protein samples and characterised pure protein samples, in conjunction with electrophoresis, are utilised to study protein structure and the potential of protein electrophoresis. Using non-denaturing and denaturing electrophoresis, students understand the difference between primary, tertiary and quaternary structures, the importance of disulfhydryl bridges in maintaining protein structure and electrophoresis in studying complete proteins and protein subunits.

Supplier: G-Biosciences

Biodiversity lab activity is designed for determination of protein contents and biomass in diverse biological samples to study how biomass is related to biodiversity in nature. Students collect and catalogue plant leaf samples from a diverse group of locally available plants. This lab activity involves determination of natural weight of each plant sample, grinding a predetermined amount of each sample, and the subsequent extraction of proteins from the samples. Students then learn to determine protein contents of each plant sample and attempt to relate the protein content with biomass. Students in this lab activity are challenged to think, analyse, and seek answers as to why protein biomasses vary for a given natural weight for different plants. Finally, they will relate that finding to the biodiversity of nature.

Supplier: Avantor

The J.T.Baker® BAKERBOND® PolyPEI™ multimode ion exchange (IEX) chromatography resin functions as a weak anion exchange resin with improved selectivity for superior separation in process chromatography applications.

Supplier: Avantor

Aluminium oxide basic, powder, BAKER ANALYZED® Brockmann Activity Grade I for chromatography, J.T.Baker®

Supplier: G-Biosciences

Dot blotting is a simple technique to identify a known protein in a biological sample. The ease and simplicity of the technique makes dot blotting an ideal diagnostic tool.

Supplier: EDVOTEK

Students learn the basic principles of the Enzyme-linked Immunosorbent Assay (ELISA) using this precise and sensitive antibody-based detection kit.

Supplier: Avantor

The J.T.Baker® BAKERBOND® PolyQUAT™ multimode ion exchange (IEX) chromatography resin functions as a strong anion exchange resin with improved selectivity for superior separation in process chromatography applications.