Fluor-de-Lys® SIRT2 fluorometric drug discovery assay kit
Supplier: Enzo Life Sciences
A Fluor-de-Lys® fluorescent assay system. The SIRT2 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human SIRT2 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions.
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Bolton-Hunter reagent (sulpho-SHPP), Pierce™
Supplier: Thermo Fisher Scientific
Sulpho-SHPP, sulphosuccinimidyl-3 -(4-hydroxypheynyl) propionate, also known as water-soluble Bolton-Hunter reagent, conjugates tyrosine-like functional groups to primary amines to increase the number of tyrosyl groups that can be iodinated by iodine-125 labeling procedures.
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Minimum essential medium (MEM) alpha medium, Corning®
Supplier: Corning
Minimum Essential Medium (MEM) is a common cell culture medium developed from early work using Basal Medium Eagle (BME) with normal mammalian fibroblasts and certain subtypes of HeLa cells.
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Protein G Mag Sepharose
Supplier: Cytiva
Protein G Mag Sepharose simplifies enrichment of target proteins by immunoprecipitation techniques, protein antigen precipitation from solutions using antibodies, or pull-down applications as an in vitro technique to detect physical interactions.
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Anti-IGF2BP2 Rabbit Polyclonal Antibody
Supplier: ProSci Inc.
This gene encodes a member of the IGF-II mRNA-binding protein (IMP) family. The protein encoded by this gene contains several four KH domains and two RRM domains. It functions by binding to the 5' UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulating IGF2 translation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
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Amersham CyDye DIGE fluors
Supplier: Cytiva
CyDye™ DIGE fluors are available as minimal and saturation labeling dyes. The minimal dyes are intended for general 2-D application use where sufficient amounts of sample are available. The saturation dyes from the Scarce Sample Labeling Kit are designed to be used for applications where only small amounts of sample are available, for example in Laser Capture Microdissection.
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SMPT (4-succinimidyloxycarbonyl-alpha-methyl-α(2-pyridyldithio)toluene), Pierce™
Supplier: Thermo Fisher Scientific
Thermo Scientific Pierce SMPT is a long-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form strong (hindered) yet cleavable (reducible) disulfide bonds with cysteine sulfhydryls..
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Human recombinant FGF21 (from E. coli)
Supplier: Shenandoah Biotechnology
Fibroblast growth factor 21 (FGF-21) is an endocrine hormone that regulates energy homeostasis and exerts cardioprotective functions during heart injury. FGF-21 is expressed in the liver, pancreas, heart, and adipose tissues. FGF-21 signaling is activated through the FGFR1c receptor and β-Klotho co-receptor. FGF-21 is an important regulator of glucose uptake and reduces cell apoptosis under stress conditions.
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SPB (Succinimidyl-[4-(psoralen-8-yloxy)]butyrate), Pierce™
Supplier: Thermo Fisher Scientific
Thermo Scientific Pierce SPB is an NHS-ester and psoralen crosslinker that effectively conjugates primary amines to DNA via light-activated intercalation next to pyrimidine bases.
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Solid phase extraction cartridges, Bond Elut™ NH₂
Supplier: VARIAN
Bond Elut™ NH₂ is a weaker anion exchanger than sorbents such as SAX (a quaternary amine sorbent that is always charged) and is therefore a better choice for retention of very strong anions, such as sulfonic acids, which may retain irreversibly on a SAX sorbent. Similar to Diol and SI sorbents, Bond Elut™ NH₂ is excellent for the separation of structural isomers.
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Organic reference standard, Glycerol, analytical standard, Supelco®
Supplier: Merck
Glycerol may be used as a reference standard in the determination of glycerol in samples of beer using gas liquid chromatography (GLC). It is a simple polyol compound, which is obtained as a by-product of soap and bio-diesel industries. It is odourless, colour-less, viscous in nature, and exists as a sweet tasting liquid. It can be derived naturally as well as from petrochemical feedstock.
Glycerol has been used as:
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Anti-CTBP2 Rabbit Polyclonal Antibody
Supplier: Boster Bio
Rabbit IgG polyclonal antibody for C-terminal-binding protein 2(CTBP2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
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Anti-MINPP1 Rabbit Polyclonal Antibody
Supplier: ProSci Inc.
This gene encodes multiple inositol polyphosphate phosphatase; an enzyme that removes 3-phosphate from inositol phosphate substrates. It is the only enzyme known to hydrolzye inositol pentakisphosphate and inositol hexakisphosphate. This enzyme also converts 2,3 bisphosphoglycerate (2,3-BPG) to 2-phosphoglycerate; an activity formerly thought to be exclusive to 2,3-BPG synthase/2-phosphatase (BPGM) in the Rapoport-Luebering shunt of the glycolytic pathway.
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Chymotrypsin™, sequencing grade
Supplier: G-Biosciences
SG-Chymotrypsin™ is a serine endopeptidase, which predominantly cleaves peptide bonds on the carboxy side of tyrosine, phenylalanine and tryptophan. In addition, chymotrypsin has a low catalytic activity against the carboxy side of leucine, methionine, alanine, aspartic and glutamic acids. It is therefore recommended to always use the shortest digestion time possible.
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Anti-PAOX Rabbit Polyclonal Antibody
Supplier: ProSci Inc.
PAOX belongs to the flavin monoamine oxidase family. PAOX is a flavoenzyme which catalyzes the oxidation of N (1)-acetylspermine to spermidine and is thus involved in the polyamine back-conversion. It can also oxidize N (1)-acetylspermidine to putrescine. PAOX does not oxidize spermidine. It plays an important role in the regulation of polyamine intracellular concentration and has the potential to act as a determinant of cellular sensitivity to the antitumor polyamine analogs.
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Anti-TNFSF13 / ADIPOQ Rat Monoclonal Antibody
Supplier: ProSci Inc.
Anti-TNFSF13 / ADIPOQ Rat Monoclonal Antibody
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NGF Rapid™ ELISA kit: rat
Supplier: Biosensis
The Biosensis NGF Rapid™ enzyme-linked immunosorbent assay (ELISA) kit is a sandwich ELISA that allows the quantification of rat NGF in less than 3 hours in cell culture supernatants, serum, and brain extracts only if used as directed.
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RPMI 1640 (1X), Cell Culture Media, MP Biomedicals
Supplier: MP Biomedicals
RPMI has successfully been used for the cultivation of normal human and neoplastic leukocytes. It is now a popular general purpose medium when properly supplemented. As a modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent.
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Anti-Neurotrophin-3 Chicken Polyclonal Antibody
Supplier: Biosensis
NT3 is a member of the neurotrophin family, that controls survival and differentiation of visceral and proprioceptive sensory neurons. NT3 is closely related to both NGF and BDNF. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NT3-deficient mice generated by gene targeting display sevvere movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family
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proBDNF Rapid™ ELISA Kit: Human, Mouse, Rat
Supplier: Biosensis
The Biosensis proBDNF Rapid™ enzyme-linked immunosorbent assay (ELISA) kit is a sandwich ELISA that allows the specific, fast and reliable quantification of proBDNF in less than 4 hours in cell culture supernatants, cell lysates, serum, citrate-plasma and tissue extracts only if used as directed.
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Anti-PAOX Rabbit Polyclonal Antibody
Supplier: ProSci Inc.
PAOX belongs to the flavin monoamine oxidase family. PAOX is a flavoenzyme which catalyzes the oxidation of N (1)-acetylspermine to spermidine and is thus involved in the polyamine back-conversion. It can also oxidize N (1)-acetylspermidine to putrescine. PAOX does not oxidize spermidine. It plays an important role in the regulation of polyamine intracellular concentration and has the potential to act as a determinant of cellular sensitivity to the antitumor polyamine analogs.
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Anti-GRIN2B Rabbit Polyclonal Antibody
Supplier: Boster Bio
Rabbit IgG polyclonal antibody for Glutamate receptor ionotropic, NMDA 2B(GRIN2B) detection. Tested with WB in Human;Mouse;Rat.
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BMPS (3-Maleimidopropionic acid N-hydroxysuccinimide ester), Pierce™
Supplier: Thermo Fisher Scientific
Thermo Scientific Pierce BMPS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a short spacer arm (5.9 Å).
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SF-4 baculo express insect medium
Supplier: BIOCONCEPT
SF-4 baculovirus expression medium allows high yield expression of proteins well above all other commercially available media. The products has no variability therefore does not require initial batch testing and batch reservation.
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HPLC columns, Cogent Diamond Hydride™
Supplier: MICROSOLV
These useful HPLC columns have a silica hydride surface similar to other TYPE-C™ phases but the uniqueness of Diamond Hydride™ is that they have a very small amount of carbon on the surface that adjusts the hydrophobicity to a small but important level. This is an excellent choice for small, polar compounds by LCMS or HPLC.
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Ampicillin, white, powder
Supplier: MP Biomedicals
Ampicillin is a semi-synthetic derivative of penicillin, active as a broad-spectrum antibiotic. It is inactivated by beta-lactamases and for this reason a beta-lactamase inhibitor should be considered when using ampicillin. Against gram-positive bacteria, ampicillin has a similar mode of action as benzylpenicillin; against gram-negative bacteria, it has a similar mode of action as chloramphenicol and tetracyclines. In E. coli it inhibits cell wall synthesis.
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Cell culture media, Dulbecco's Modified Eagles Medium (DMEM), High Glucose, HyClone™
Supplier: HyClone products (Cytiva)
HyClone™ DMEM high glucose liquid media supports cell growth in research, upstream process development, and cell culture manufacturing processes.
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α-Amylase Inhibitor Screening Kit
Supplier: Abcam
Simple, 1-step assay for screening potential α-amylase inhibitors.
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Anti-Neurotrophin-4/5; NT4/NT5 Chicken Polyclonal Antibody
Supplier: Biosensis
NT4 is a member of the neurotrophin family, in knockout animals NT4 deficient mice appeared little changed from wildtype animals, however NT4 is a powerful neurotrophic factor for periferial neuronal ganglia and is capable if sustaining ganglia neurons in vitro. NT4 is closely related to both NGF and BDNF. FUNCTION: Target-derived survival factor for peripheral sensory sympathetic neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues. SIMILARITY: Belongs to the NGF-beta family.
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Peroxidase, MP Biomedicals
Supplier: MP Biomedicals
HRP is a plant glycohemoprotein belongs to the ferroprotoporphyrin group of peroxidases. It is used in biochemistry applications such as western blots, ELISA and Immunohistochemistry. It is used to amplify a weak signal and increase detectability of a target molecule, such as a protein. HRP labeled immunoglobulins are used as probes for the demonstration of tissue antigens and in enzyme immunoassay (EIA) systems for determination of soluble and insoluble antigens.