15888 Results for: "5-Carboxyfluorescein-N-succinimidyl+ester+(5-FAM+SE)"

Supplier: AAT BIOQUEST

The amino-reactive Protonex™ Green 500 SE (#21216) was developed for preparing Protonex™ Green 500 conjugates as fluorescent pH probes.

Supplier: Thermo Fisher Scientific

Thermo Scientific Pierce SMCC is a hetero-bifunctional crosslinker that contain N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines at pH 7–9 to form amide bonds, while maleimides react with sulfhydryl groups at pH 6.5–7.5 to form stable thioether bonds. In aqueous solutions, NHS ester hydrolytic degradation is a competing reaction whose rate increases with pH. The maleimide group is more stable than the NHS-ester group, but will slowly hydrolyze and lose its reaction specificity for sulfhydryls at pH values > 7.5. For these reasons, conjugations with these crosslinkers are usually performed at pH 7.2–7.5, with the NHS ester (amine-targeted) reacted before or simultaneous with the maleimide (sulfhydryl-targeted) reaction.

Supplier: Thermo Fisher Scientific

Thermo Scientific Pierce SMPB is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length aromatic spacer arm (11.6 angstroms).

Supplier: Thermo Fisher Scientific

Thermo Scientific Pierce DSP (Lomant's Reagent) is a water-insoluble, homo-bifunctional N-hydroxysuccimide ester (NHS ester) crosslinker that is thiol-cleavable, primary amine-reactive, and useful for many applications. DSP contains an amine-reactive NHS ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7–9 to form stable amide bonds and releasing N-hydroxy-succinimide. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS ester crosslinking reagents. DSP is non-sulfonated and insoluble in water, so it must first be dissolved in an organic solvent and then added to the aqueous reaction mixture. Because DSP does not possess a charged group, it is lipophilic and membrane-permeable and so useful for intracellular and intramembrane conjugation. A sulfonated analog of DSP (DTTSP) is water soluble. DSS, the non-cleavable analog of the DSP crosslinker is also available for applications that require a stable spacer arm that cannot be cleaved in the presence of reducing agents.

Supplier: AAT BIOQUEST

mFluor™ Violet 530 dyes have fluorescence excitation and emission maxima of ~405 nm and ~530 nm respectively.

Supplier: AAT BIOQUEST

mFluor™ Blue 585 dyes are an excellent alternative to RPE since they have the spectral properties equivalent to those of RPE conjugates.

Supplier: AAT BIOQUEST

mFluor™ Blue 615 dye can be well excited with blue laser at 488 nm.

Supplier: AAT BIOQUEST

Although Texas Red® is the most popular labeling reagent of sulfonyl chloride, it is quite unstable in water.

Supplier: Apollo Scientific

Spacer arm SPDPantibody-toxin conjugation

Supplier: Thermo Fisher Scientific

Thermo Scientific Pierce SPDP is a short-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable (reducible) disulfide bonds with cysteine sulfhydryls.

Supplier: AAT BIOQUEST

DABCYL, SE is the amino-reactive form of DABCYL, and widely used to prepare a variety of FRET-based probes that contain DABCYL.

Supplier: AAT BIOQUEST

An amine-reactive building block for developing a probe that can be recognized by anti-DNP antibodies; Also an excellent amine-reactive FRET quencher paired with Trp or Tyr.

Supplier: G-Biosciences

(Bis(2-[Succinimidooxycarbonyloxy]ethyl) sulfone Homobifunctional Cross Linkers.

Supplier: Merck Millipore (Calbiochem‎)

A useful agent for introducing thiol groups, in protected form, into various proteins. Reacts with primary amines to add protected sulfhydryl groups. When treated with hydroxylamine, yields a free sulfhydryl group that can be conjugated to maleimide-modified proteins.

Supplier: Thermo Fisher Scientific

Thermo Scientific Pierce SBAP is a short (6.2 angstrom) crosslinker for amine-to-sulfhydryl conjugation via N-hydroxysuccinimide (NHS) ester and bromoacetyl reactive groups.

Supplier: Thermo Fisher Scientific

Thermo Scientific Pierce SIAB is a mid-length (10.6 angstrom) crosslinker for amine-to-sulfhydryl conjugation via N-hydroxysuccinimide (NHS) ester and iodoacetyl reactive groups.

Supplier: Thermo Fisher Scientific

Thermo Scientific Pierce DSP (Lomant's Reagent) is a water-insoluble, homo-bifunctional N-hydroxysuccimide ester (NHS ester) crosslinker that is thiol-cleavable, primary amine-reactive, and useful for many applications. DSP contains an amine-reactive NHS ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7–9 to form stable amide bonds and releasing N-hydroxy-succinimide. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS ester crosslinking reagents. DSP is non-sulfonated and insoluble in water, so it must first be dissolved in an organic solvent and then added to the aqueous reaction mixture. Because DSP does not possess a charged group, it is lipophilic and membrane-permeable and so useful for intracellular and intramembrane conjugation. A sulfonated analog of DSP (DTTSP) is water soluble. DSS, the non-cleavable analog of the DSP crosslinker is also available for applications that require a stable spacer arm that cannot be cleaved in the presence of reducing agents.

Supplier: Thermo Fisher Scientific

SATP (and SATA) are reagents for introducing protected sulfhydryls into proteins, peptides and other molecules. for covalent modification of primary amines and addition of a protected sulphhydryl group.

Supplier: AAT BIOQUEST

Succinimidyl 4,4-azipentanoate, also known as NHS-Diazirine, represents a significant breakthrough in crosslinking technology.

Supplier: Thermo Fisher Scientific

Thermo Scientific Pierce SMCC is a hetero-bifunctional crosslinker that contain N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines at pH 7–9 to form amide bonds, while maleimides react with sulfhydryl groups at pH 6.5–7.5 to form stable thioether bonds. In aqueous solutions, NHS ester hydrolytic degradation is a competing reaction whose rate increases with pH. The maleimide group is more stable than the NHS-ester group, but will slowly hydrolyze and lose its reaction specificity for sulfhydryls at pH values > 7.5. For these reasons, conjugations with these crosslinkers are usually performed at pH 7.2–7.5, with the NHS ester (amine-targeted) reacted before or simultaneous with the maleimide (sulfhydryl-targeted) reaction.

Supplier: Merck Millipore (Calbiochem‎)

Heterobifunctional reagent useful for enzyme labeling of antibodies and antibody fragments. Cross-links both a sulfhydryl group and a free amine group. Useful for sequential conjugation to minimize polymerization.
Reacts with primary amines and sulfhydryl groups.

Supplier: Thermo Fisher Scientific

Thermo Scientific Pierce Premium Grade DSP is our highest quality formulation of this popular amine-reactive, cleavable crosslinker, specially characterized for applications where product integrity and risk minimization are paramount.

Supplier: Thermo Fisher Scientific

Thermo Scientific Pierce LC-SPDP is a long-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable (reducible) disulfide bonds with cysteine sulfhydryls.

Supplier: Thermo Fisher Scientific

Sulpho-SMCC (3-Sulpho-N-succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate sodium salt) ≥97%

Supplier: Thermo Fisher Scientific

Thermo Scientific Pierce SMPH is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a long spacer arm (14.2 angstroms).

Supplier: Thermo Fisher Scientific

Thermo Scientific Pierce SPB is an NHS-ester and psoralen crosslinker that effectively conjugates primary amines to DNA via light-activated intercalation next to pyrimidine bases.

Supplier: Apollo Scientific

L-Tryptophan benzyl ester

Supplier: Merck

Selenium disulphide for synthesis, Sigma-Aldrich®

Supplier: Apollo Scientific

N-Carbobenzoxyglycine methyl ester 95%

Supplier: Thermo Fisher Scientific

BAPTA tetraethyl ester ≥98%