RIPA lysis and extraction buffer, Pierce™

The RIPA lysis and extraction buffer is a high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent for cultured mammalian cells.

• Compatible with many applications, including reporter assays, protein assays, immunoassays and protein purification
• Enables extraction of cytoplasmic, membrane and nuclear proteins making it very versatile
• Contains no proprietary components, providing users with complete control and knowledge of possible compatibility issues

This buffer effectively lyses and extracts protein from cultured mammalian cells, including plated cells and pelleted suspension cells. The reagent enables the extraction of membrane, nuclear and cytoplasmic proteins and is compatible with many applications, including reporter assays, immunoassays and protein purification. The cell lysis reagent is highly effective for protein extraction from a variety of cell types because it contains three non-ionic and ionic detergents.