High-throughput DNA and RNA isolation from cultured cells and tissue using magnetic beads.

• DNA/RNA Co-extraction – DNA and RNA purification into two fractions

• NGS-ready – High-quality DNA and RNA with high DIN and RIN scores
• Automation-ready – Automatable on open liquid handlers and most magnetic processors

The Mag-Bind® DNA/RNA Kit is designed for simultaneous purification of DNA and RNA up to 1×106 cells and up to 10 mg tissue samples into two separate eluates from the same sample. The Kit is also capable of separate purification of miRNA fraction using an optional protocol. Extraction of DNA and RNA allows for comprehensive molecular analysis from the same sample source since there is no need for sample splitting.

The protocol uses an innovative Lysis/Binding master mix that conveniently lyses the sample first and upon addition of Mag-Bind® Particles CH binds the DNA to the particles. The RNA-containing supernatant is saved, and an RNA binding step is completed in the presence of binding buffer and isopropanol to bind RNA (>200 nt) to the second set of Mag-Bind® Particles CH. This results in separation of DNA and RNA into two fractions. The RNA-fraction is further treated with DNase I enzyme to digest the residual DNA and rebound to the same set of Mag-Bind® Particles CH by adjusting the binding conditions. Mag-Bind® Particles CH bound to DNA and RNA are individually washed and nucleic acids are eluted in two different tubes/microplates for further analyses. The purified DNA and RNA is ready for use in a wide range of downstream applications such as NGS, PCR, qPCR, RT-qPCR, and more.

The Mag-Bind® DNA/RNA Kit utilises magnetic bead-based technology and can be processed either manually or automated on most open-ended liquid handling platforms as well as magnetic processors. Please follow the instructions outlined in the manual for tissues stored in preservation reagents such as DNA/RNA Shield™ and RNAlater™.