Human Neuron specific Enolase ELISA kit is a single-wash 90-min Simplestep used to quantify Human Neuron specific Enolase with a sensitivity of 23 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. Colorimetric Sandwich ELISA - 450 nm readout: works on any standard plate reader. Validated on a number of sample types including cerebrospinal fluid (CSF).