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Specifications
- Assay duration:Multiple steps
- Assay Type:Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Mouse
- Target protein:SDF1
- Description:Mouse SDF1 ELISA kit (small sample volume)
- Sample type:Serum, plasma, and other biological fluids
- Cross reactivity:No significant cross-reactivity or interference between mouse SDF1 and analogues was observed.
- Detection method:Colorimetric
- Time to Results:3 h 30 min
- Shelf life:Store for 6 months at 4 °C
- Detection range:78,13 - 5000 pg/ml
- Storage temperature:4 °C
- Sensitivity:32,15 pg/ml
- Regulatory status:RUO
- Tests per kit:96 tests
Specifications
About this item
Mouse SDF1 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse SDF1 in serum, plasma, and other biological fluids. This ELISA kit only requires 25 µl of sample volume(s).
- Small Sample Volume: Requires as little as 25 µl of sample!
- Assay Precision: Intra-Assay: CV <10%, Inter-Assay: CV <10%
Mouse SDF1 ELISA kit employs the sandwich Enzyme-Linked Immunoassay technique for the quantitative measurement of mouse SDF1 in serum, plasma, and other biological fluids. An antibody specific for SDF1 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the SDF1 present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-SDF1 Antibody, which binds the captured SDF1 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Avidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of SDF1 captured in each well. The concentration of SDF1 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.