Some Products May Appear Restricted
To ensure a smooth and speedy checkout, please log in to your account. Some items may show as restricted simply because you're not logged in.
If you do not have an account, you can register using our registration webform (https://www.avantorsciences.com/us/en/login/register)
If you're still seeing restrictions after logging in, certain products—like chemicals or medical devices—require additional account verification steps to be able to place an order. Some items may additionally require a specific license or customer documentation; additional documentation will be requested for these items prior to shipment.
- Media Format:Liquid
- Pk:500 mL
- Description:Microvascular endothelial cell growth medium, basal
- Media Base:Basal Medium
Microvascular Endothelial Cell Growth Medium, Advanced, is a sterile liquid culture medium designed for cultivating human microvascular endothelial cells (HMVEC). Delivered as a basal medium, it is suitable for culturing Provitro HMVEC upon the addition of supplement mix components.
- Formulation is optimised for the initial seeding of 4000 cells/cm², allowing growth up to confluence (approximately 90%)
- Use of feeder layers, matrix substrates, or additional substances is unnecessary
- Due to the possibility of reduced proliferative activity, it is recommended to use the antibiotic supplement for freshly isolated cells only
Due to the possibility of reduced proliferative activity, it is recommended to use the antibiotic supplement for freshly isolated cells only. Endothelial cell proliferation medium is thoroughly tested after each production. All components are tested in a stringent biological assay.
Each batch is checked for human endothelial cells proliferating characteristics. The cells cultured in endothelial cell growth medium are checked regarding their morphology, the adherence rate, the colony forming efficiency, and the population doubling time.
Storge: Store the supplemented endothelial cell proliferation medium in a dark environment at temperatures ranging from 4 to 8 °C for a maximum duration of one month. Avoid heating the medium beyond 37 °C and refrain from using uncontrolled heat sources, such as microwave appliances. When utilising a portion of the medium, extract the required amount from the bottle and warm it appropriately. Never freeze the medium, as this could result in elevated salt concentrations due to the separation of pure water during freezing, leading to irreversible damage.