Some Products May Appear Restricted
To ensure a smooth and speedy checkout, please log in to your account. Some items may show as restricted simply because you're not logged in.
If you do not have an account, you can register using our registration webform (https://www.avantorsciences.com/us/en/login/register)
If you're still seeing restrictions after logging in, certain products—like chemicals or medical devices—require additional account verification steps to be able to place an order. Some items may additionally require a specific license or customer documentation; additional documentation will be requested for these items prior to shipment.
- Pk:96 Tests
- Assay duration:Multiple steps
- Assay Type:Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Rat
- Target protein:Complement Factor B
- Description:Rat complement factor B ELISA kit
- Sample type:Serum, plasma, tissue homogenates and other biological fluids
- Cross reactivity:Rat Complement Factor B ELISA kit exhibits high specificity and excellent specificity for the detection of rat Complement Factor B. No significant cross-reactivity or interference between Complement Factor B and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf life:Store for 6 months at 4 °C
- Detection range:3,125 - 200 ng/ml
- Storage temperature:4 °C
- Sample volume:100 μl
- Sensitivity:1,875 ng/ml
- Regulatory status:RUO
Rat Complement Factor B ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat Complement Factor B in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-to-use ELISA Kit
- Detection range: 3,125 to 200 ng/ml
- Sensitivity: 1,875 ng/ml
- Sample type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Rat Complement Factor B ELISA kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of rat Complement Factor B in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Complement Factor B has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Complement Factor B present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Complement Factor B Antibody, which binds the captured Complement Factor B present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Complement Factor B captured in each well. The concentration of Complement Factor B can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.