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Specifications
- Pk:96 Tests
- Assay duration:Multiple steps
- Assay Type:Competitive
- Format:Pre-coated
- Primary antibody reactivity:Rat
- Target protein:Bradykinin
- Description:Rat bradykinin ELISA kit
- Sample type:Serum, plasma, tissue homogenates and other biological fluids
- Cross reactivity:Rat Bradykinin ELISA Kit exhibits high specificity and excellent specificity for the detection of rat Bradykinin. No significant cross-reactivity or interference between Bradykinin and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf life:Store for 6 months at 4 °C
- Detection range:7,813 - 500 pg/ml
- Storage temperature:4 °C
- Sample volume:50 μl
- Sensitivity:4,688 pg/ml
- Regulatory status:RUO
Specifications
About this item
Rat Bradykinin ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of rat Bradykinin in serum, plasma, tissue homogenates, and other biological fluids.
- Ready to use ELISA kit
- Detection range: 7,813 to 500 pg/ml
- Sensitivity: 4,688 pg/ml
- Sample type: Serum, plasma, tissue homogenates and other biological fluids
- Assay precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%
Rat Bradykinin ELISA kit employs the competitive enzyme immunoassay technique for the quantitative measurement of rat Bradykinin in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with Bradykinin antigen. During the incubation, Bradykinin present in the samples or standards competes with the fixed amount of immobilised Bradykinin for binding sites on the Biotinylated Anti-Bradykinin Antibody. The more Bradykinin present in a sample or standard, the less Biotinylated Anti-Bradykinin Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Bradykinin Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Bradykinin present in each sample or standard. The concentration of Bradykinin can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.