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Specifications
- Pk:96 Tests
- Assay duration:Multiple steps
- Assay Type:Competitive
- Format:Pre-coated
- Primary antibody reactivity:Goat
- Target protein:Luteinizing Hormone
- Description:Goat Luteinizing Hormone ELISA kit
- Sample type:Serum, plasma, tissue homogenates and other biological fluids
- Cross reactivity:Goat Luteinizing Hormone ELISA Kit exhibits high specificity and excellent specificity for the detection of goat Luteinizing Hormone. No significant cross-reactivity or interference between Luteinizing Hormone and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf life:Store for 6 months at 4 °C
- Detection range:1,563 - 100 mIU/ml
- Storage temperature:4 °C
- Sample volume:50 μl
- Sensitivity:0,938 mIU/ml
- Regulatory status:RUO
Specifications
About this item
Goat Luteinizing Hormone ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of goat Luteinizing Hormone in serum, plasma, tissue homogenates, and other biological fluids.
- Ready to use ELISA Kit
- Detection range: 1,563 to 100 mIU/ml
- Sensitivity: 0,938 mIU/ml
- Sample type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%
Goat Luteinizing Hormone ELISA Kit employs the competitive enzyme immunoassay technique for the quantitative measurement of goat Luteinizing Hormone in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with Luteinizing Hormone antigen. During the incubation, Luteinizing Hormone present in the samples or standards competes with the fixed amount of immobilised Luteinizing Hormone for binding sites on the Biotinylated Anti-Luteinizing Hormone Antibody. The more Luteinizing Hormone present in a sample or standard, the less Biotinylated Anti-Luteinizing Hormone Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Luteinizing Hormone Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Luteinizing Hormone present in each sample or standard. The concentration of Luteinizing Hormone can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.