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Specifications
- Pk:96 Tests
- Assay duration:Multiple steps
- Assay Type:Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Rat
- Target protein:Vitamin K-dependent Protein C
- Description:Rat vitamin K-dependent protein C ELISA kit
- Sample type:Serum, plasma, tissue homogenates and other biological fluids
- Cross reactivity:Rat Vitamin K-dependent Protein C ELISA kit exhibits high specificity and excellent specificity for the detection of rat Vitamin K-dependent Protein C. No significant cross-reactivity or interference between Vitamin K-dependent Protein C and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf life:Store for 6 months at 4 °C
- Detection range:1,563 - 100 ng/ml
- Storage temperature:4 °C
- Sample volume:100 μl
- Sensitivity:0,938 ng/ml
- Regulatory status:RUO
Specifications
About this item
Rat Vitamin K-dependent Protein C ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat Vitamin K-dependent Protein C in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-to-use ELISA Kit
- Detection range: 1,563 to 100 ng/ml
- Sensitivity: 0,938 ng/ml
- Sample type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Rat Vitamin K-dependent Protein C ELISA kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of rat Vitamin K-dependent Protein C in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Vitamin K-dependent Protein C has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Vitamin K-dependent Protein C present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Vitamin K-dependent Protein C Antibody, which binds the captured Vitamin K-dependent Protein C present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Vitamin K-dependent Protein C captured in each well. The concentration of Vitamin K-dependent Protein C can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.