Bovine Thyroxine/T4 ELISA Kit

Bovine thyroxine/T4 ELISA kit, 96 Tests

ANTIA87529-96

ANTIA87529-96

£532.00

Bovine Thyroxine/T4 ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of bovine Thyroxine/T4 in serum, plasma, tissue homogenates, and other biological fluids.

Ready to use ELISA kit
Detection range: 0,703 to 45 ng/ml
Sensitivity: 0,422 ng/ml
Sample type: Serum, plasma, tissue homogenates and other biological fluids
Assay precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%

Bovine Thyroxine/T4 ELISA kit employs the competitive enzyme immunoassay technique for the quantitative measurement of bovine Thyroxine/T4 in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with Thyroxine/T4 antigen. During the incubation, Thyroxine/T4 present in the samples or standards competes with the fixed amount of immobilised Thyroxine/T4 for binding sites on the Biotinylated Anti-Thyroxine/T4 Antibody. The more Thyroxine/T4 present in a sample or standard, the less Biotinylated Anti-Thyroxine/T4 Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Thyroxine/T4 Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Thyroxine/T4 present in each sample or standard. The concentration of Thyroxine/T4 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.

: This is a ready to use ELISA kit that contains: Pre-coated 96 well microplate, lyophilised standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-Streptavidin conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.

Pk:
96 Tests
Description:
Bovine thyroxine/T4 ELISA kit
Primary antibody reactivity:
Bovine
Sample type:
Serum, plasma, tissue homogenates and other biological fluids
Sample volume:
50 μl
Detection range:
0,703 - 45 ng/ml
Storage temperature:
4 °C
Shelf life:
Store for 6 months at 4 °C
Assay Type:
Competitive
Time to Results:
4 h 30 min
Cross reactivity:
Bovine Thyroxine/T4 ELISA Kit exhibits high specificity and excellent specificity for the detection of bovine Thyroxine/T4. No significant cross-reactivity or interference between Thyroxine/T4 and analogues was observed.
Assay Principle:
Quantitative
Cat. No.:
ANTIA87529-96
Assay duration:
Multiple steps
Detection method:
Colorimetric
Sensitivity:
0,422 ng/ml
Format:
Pre-coated
Target protein:
Thyroxine/T4
Regulatory status:
RUO

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