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Specifications
- Pk:96 Tests
- Assay duration:Multiple steps
- Assay Type:Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Rat
- Target protein:SLC31A1/CTR1
- Description:Rat SLC31A1/CTR1 ELISA kit
- Sample type:Serum, plasma, tissue homogenates and other biological fluids
- Cross reactivity:Rat SLC31A1/CTR1 ELISA kit exhibits high specificity and excellent specificity for the detection of rat SLC31A1/CTR1. No significant cross-reactivity or interference between SLC31A1/CTR1 and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf life:Store for 6 months at 4 °C
- Detection range:0,313 - 20 ng/ml
- Storage temperature:4 °C
- Sample volume:100 μl
- Sensitivity:0,188 ng/ml
- Regulatory status:RUO
Specifications
About this item
Rat SLC31A1/CTR1 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat SLC31A1/CTR1 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready to use ELISA kit
- Detection range: 0,313 to 20 ng/ml
- Sensitivity: 0,188 ng/ml
- Sample type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay precision: Intra assay: CV <8%, Inter assay: CV <10%
Rat SLC31A1/CTR1 employs the sandwich enzyme immunoassay technique for the quantitative measurement of rat SLC31A1/CTR1 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for SLC31A1/CTR1 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the SLC31A1/CTR1 present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-SLC31A1/CTR1 Antibody, which binds the captured SLC31A1/CTR1 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of SLC31A1/CTR1 captured in each well. The concentration of SLC31A1/CTR1 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.