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- Pk:96 Tests
- Assay duration:Multiple steps
- Assay Type:Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Rat
- Target protein:HIF-1 alpha
- Description:Rat HIF-1 alpha ELISA kit
- Sample type:Serum, plasma, tissue homogenates and other biological fluids
- Cross reactivity:Rat HIF-1 alpha ELISA kit exhibits high specificity and excellent specificity for the detection of rat HIF-1 alpha. No significant cross-reactivity or interference between HIF-1 alpha and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf life:Store for 6 months at 4 °C
- Detection range:7,813 - 500 pg/ml
- Storage temperature:4 °C
- Sample volume:100 μl
- Sensitivity:4,688 pg/ml
- Regulatory status:RUO
Rat HIF-1 alpha ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat HIF-1 alpha in serum, plasma, tissue homogenates, and other biological fluids.
- Ready to use ELISA kit
- Detection range: 7,813 to 500 pg/ml
- Sensitivity: 4,688 pg/ml
- Sample type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay precision: Intra assay: CV <8%, Inter assay: CV <10%
Rat HIF-1 alpha employs the sandwich enzyme immunoassay technique for the quantitative measurement of rat HIF-1 alpha in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for HIF-1 alpha has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the HIF-1 alpha present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-HIF-1 alpha Antibody, which binds the captured HIF-1 alpha present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of HIF-1 alpha captured in each well. The concentration of HIF-1 alpha can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.