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Specifications
- Pk:96 Tests
- Assay duration:Multiple steps
- Assay Type:Competitive
- Format:Pre-coated
- Primary antibody reactivity:Other
- Target protein:Ghrelin
- Description:Camel Ghrelin ELISA kit
- Sample type:Serum, plasma, tissue homogenates, and other biological fluids
- Cross reactivity:Camel Ghrelin ELISA kit exhibits high specificity and excellent specificity for the detection of camel Ghrelin. No significant cross-reactivity or interference between Ghrelin and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf life:Store for 6 months at 4 °C
- Detection range:7,813 - 500 pg/ml
- Storage temperature:4 °C
- Sample volume:50 μl
- Sensitivity:4,688 pg/ml
- Regulatory status:RUO
Specifications
About this item
Camel Ghrelin ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of camel Ghrelin in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-to-use ELISA kit
- Detection range: 7,813 to 500 pg/ml
- Sensitivity: 4,688 pg/ml
- Sample type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%
Camel Ghrelin ELISA kit employs the competitive enzyme immunoassay technique for the quantitative measurement of camel Ghrelin in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with Ghrelin antigen. During the incubation, Ghrelin present in the samples or standards competes with the fixed amount of immobilised Ghrelin for binding sites on the Biotinylated Anti-Ghrelin antibody. The more Ghrelin present in a sample or standard, the less Biotinylated Anti-Ghrelin antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Ghrelin antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Ghrelin present in each sample or standard. The concentration of Ghrelin can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.