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Specifications
- Pk:96 Tests
- Assay duration:One step
- Assay Type:Sandwich
- Format:Pre-coated
- Host:
- Primary antibody reactivity:Mouse
- Target protein:Gastrokine 1
- Description:Mouse Gastrokine 1 ELISA kit
- Environmentally Preferable:
- Sample type:Serum, plasma or other biological fluids
- Detection method:Colorimetric
- Time to Results:1 h 30 min
- Shelf life:Store for 6 months at 4 °C
- Detection range:0,75 - 12 ng/ml
- Storage temperature:4 °C
- Sample volume:40 μl
- Sensitivity:0,024 ng/ml
- Regulatory status:RUO
Specifications
About this item
Mouse Gastrokine 1 ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse Gastrokine 1 in serum, plasma, and other biological fluids.
- Higher throughput: Get results in just 90 minutess, with a single wash step
- Detection Range: 0,75 to 12 ng/ml
- Sensitivity: 0,024 ng/ml
- Sample Type: Serum, plasma or other biological fluids
- Assay Precision: Intra-assay: CV <8%, Inter-assay: CV <10%
Mouse Gastrokine 1 ELISA kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse Gastrokine 1 in serum, plasma or other biological fluids. An antibody specific for Gastrokine 1 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Gastrokine 1 present in each sample is bound to the wells by the immobilised antibody. Biotinylated anti-Gastrokine 1 antibody, which also binds the Gastrokine 1 present in each sample, and Streptavidin-HRP, which binds the Biotinylated anti-Gastrokine 1 antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated anti-Gastrokine 1 antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Colour develops in proportion to the amount of Gastrokine 1 captured in each well. The colour development is stopped by addition of stop solution which changes the colour from blue to yellow and the intensity of the colour is then measured. The concentration of Gastrokine 1 in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.