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Specifications
- Pk:96 Tests
- Assay duration:One step
- Assay Type:Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Human
- Target protein:Semaphorin 3A
- Description:Human Semaphorin 3A ELISA kit
- Sample type:Serum, plasma or other biological fluids
- Detection method:Colorimetric
- Time to Results:1 h 30 min
- Shelf life:Store for 6 months at 4 °C
- Detection range:0,75 - 12 ng/ml
- Storage temperature:4 °C
- Sample volume:40 μl
- Sensitivity:0,024 ng/ml
- Regulatory status:RUO
Specifications
About this item
Human Semaphorin 3A ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Semaphorin 3A in serum, plasma, and other biological fluids.
- Higher throughput: Get results in just 90 minutes, with a single wash step
- Detection range: 0,75 to 12 ng/ml
- Sensitivity: 0,024 ng/ml
- Sample type: Serum, plasma or other biological fluids
- Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%
Human Semaphorin 3A ELISA kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Semaphorin 3A in serum, plasma or other biological fluids. An antibody specific for Semaphorin 3A has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Semaphorin 3A present in each sample is bound to the wells by the immobilised antibody. Biotinylated Anti-Semaphorin 3A antibody, which also binds the Semaphorin 3A present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-Semaphorin 3A antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-Semaphorin 3A antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Colour develops in proportion to the amount of Semaphorin 3A captured in each well. The colour development is stopped by addition of stop solution which changes the colour from blue to yellow and the intensity of the colour is then measured. The concentration of Semaphorin 3A in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.