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Specifications
- Pk:96 Tests
- Assay duration:One step
- Assay Type:Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Human
- Target protein:GITR
- Description:Human GITR ELISA kit
- Sample type:Serum, plasma or other biological fluids
- Detection method:Colorimetric
- Time to Results:1 h 30 min
- Shelf life:Store for 6 months at 4 °C
- Detection range:3 - 48 ng/ml
- Storage temperature:4 °C
- Sample volume:40 μl
- Sensitivity:0,154 ng/ml
- Regulatory status:RUO
Specifications
About this item
Human GITR ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human GITR in serum, plasma, and other biological fluids.
- Higher throughput: Get results in just 90 minutess, with a single wash step
- Detection Range: 3 to 48 ng/ml
- Sensitivity: 0,154 ng/ml
- Sample Type: Serum, plasma or other biological fluids
- Assay Precision: Intra-assay: CV <8%, Inter-assay: CV <10%
Human GITR ELISA kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of human GITR in serum, plasma or other biological fluids. An antibody specific for GITR has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the GITR present in each sample is bound to the wells by the immobilised antibody. Biotinylated anti-GITR antibody, which also binds the GITR present in each sample, and Streptavidin-HRP, which binds the Biotinylated anti-GITR antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated anti-GITR antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Colour develops in proportion to the amount of GITR captured in each well. The colour development is stopped by addition of stop solution which changes the colour from blue to yellow and the intensity of the colour is then measured. The concentration of GITR in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.