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Specifications
- Pk:96 Tests
- Assay duration:One step
- Assay Type:Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Mouse
- Target protein:Amyloid Precursor Protein
- Description:Mouse amyloid precursor protein ELISA kit
- Sample type:Serum, plasma or other biological fluids
- Detection method:Colorimetric
- Time to Results:1 h 30 min
- Shelf life:Store for 6 months at 4 °C
- Detection range:75 - 1200 ng/ml
- Storage temperature:4 °C
- Sample volume:40 μl
- Sensitivity:5,36 ng/ml
- Regulatory status:RUO
Specifications
About this item
Mouse Amyloid Precursor Protein ELISA kit is a 90 minute sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse Amyloid Precursor Protein in serum, plasma, and other biological fluids.
- Higher throughput: Get results in just 90 minutes, with a single wash step
- Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%
Mouse Amyloid Precursor Protein ELISA kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse Amyloid Precursor Protein in serum, plasma or other biological fluids. An antibody specific for Amyloid Precursor Protein has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Amyloid Precursor Protein present in each sample is bound to the wells by the immobilised antibody. Biotinylated anti-Amyloid Precursor Protein antibody, which also binds the Amyloid Precursor Protein present in each sample, and Streptavidin-HRP, which binds the Biotinylated anti-Amyloid Precursor Protein antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated anti-Amyloid Precursor Protein antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Colour develops in proportion to the amount of Amyloid Precursor Protein captured in each well. The colour development is stopped by addition of stop solution which changes the colour from blue to yellow and the intensity of the colour is then measured. The concentration of Amyloid Precursor Protein in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.