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* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. Avantor will not lift restrictions for residential shipping addresses.
- Pk:96 Tests
- Assay duration:One step
- Assay Type:Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Human
- Target protein:SBCAD
- Description:Human SBCAD ELISA kit
- Sample type:Serum, plasma or other biological fluids
- Detection method:Colorimetric
- Time to Results:1 h 30 min
- Shelf life:Store for 6 months at 4 °C
- Detection range:150 - 2400 ng/L
- Storage temperature:4 °C
- Sample volume:40 μl
- Sensitivity:9,63 ng/L
- Regulatory status:RUO
Human SBCAD ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human SBCAD in serum, plasma, and other biological fluids.
- Higher throughput: Get results in just 90 minutes, with a single wash step
- Detection range: 150 to 2400 ng/L
- Sensitivity: 9,63 ng/L
- Sample type: Serum, plasma or other biological fluids
- Assay precision: Intra assay: CV <8%, Inter assay: CV <10%
Human SBCAD ELISA kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of human SBCAD in serum, plasma or other biological fluids. An antibody specific for SBCAD has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the SBCAD present in each sample is bound to the wells by the immobilised antibody. Biotinylated Anti-SBCAD antibody, which also binds the SBCAD present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-SBCAD antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-SBCAD antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. colour develops in proportion to the amount of SBCAD captured in each well. The colour development is stopped by addition of stop solution which changes the colour from blue to yellow and the intensity of the colour is then measured. The concentration of SBCAD in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.