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Specifications
- Pk:96 Tests
- Assay duration:Multiple steps
- Assay Type:Competitive
- Format:Pre-coated
- Primary antibody reactivity:Chicken
- Target protein:IGFBP3
- Description:Chicken IGFBP3 ELISA kit
- Sample type:Serum, plasma, tissue homogenates and other biological fluids
- Cross reactivity:Chicken IGFBP3 ELISA kit exhibits high specificity and excellent specificity for the detection of chicken IGFBP3. No significant cross-reactivity or interference between IGFBP3 and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf life:Store for 6 months at 4 °C
- Detection range:6,25 - 400 ng/ml
- Storage temperature:4 °C
- Sample volume:50 μl
- Sensitivity:3,75 ng/ml
- Regulatory status:RUO
Specifications
About this item
Chicken IGFBP3 ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of chicken IGFBP3 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-to-use ELISA kit
- Detection Range: 6,25 to 400 ng/ml
- Sensitivity: 3,75 ng/ml
- Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Chicken IGFBP3 ELISA kit employs the competitive enzyme immunoassay technique for the quantitative measurement of chicken IGFBP3 in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with IGFBP3 antigen. During the incubation, IGFBP3 present in the samples or standards competes with the fixed amount of immobilised IGFBP3 for binding sites on the Biotinylated Anti-IGFBP3 Antibody. The more IGFBP3 present in a sample or standard, the less Biotinylated Anti-IGFBP3 Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-IGFBP3 Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of IGFBP3 present in each sample or standard. The concentration of IGFBP3 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.