To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. Avantor will not lift restrictions for residential shipping addresses.
- Pk:96 Tests
- Assay duration:One step
- Assay Type:Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Human
- Target protein:EZH2
- Description:Human EZH2 ELISA kit
- Sample type:Serum, plasma or other biological fluids
- Detection method:Colorimetric
- Time to Results:1 h 30 min
- Shelf life:Store for 6 months at 4 °C
- Detection range:150 - 2400 ng/L
- Storage temperature:4 °C
- Sample volume:40 μl
- Sensitivity:9,93 ng/L
- Regulatory status:RUO
Human EZH2 ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human EZH2 in serum, plasma, and other biological fluids.
- Higher throughput: Get results in just 90 minutess, with a single wash step
- Detection Range: 150 to 2400 ng/L
- Sensitivity: 9,93 ng/L
- Sample Type: Serum, plasma or other biological fluids
- Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Human EZH2 ELISA kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of human EZH2 in serum, plasma or other biological fluids. An antibody specific for EZH2 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the EZH2 present in each sample is bound to the wells by the immobilised antibody. Biotinylated anti-EZH2 antibody, which also binds the EZH2 present in each sample, and Streptavidin-HRP, which binds the Biotinylated anti-EZH2 antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated anti-EZH2 antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. colour develops in proportion to the amount of EZH2 captured in each well. The colour development is stopped by addition of stop solution which changes the colour from blue to yellow and the intensity of the colour is then measured. The concentration of EZH2 in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.