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- Pk:96 Tests
- Assay duration:Multiple steps
- Assay Type:Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Human
- Target protein:TMEM132D
- Description:Human TMEM132D ELISA kit
- Sample type:Serum, plasma, tissue homogenates and other biological fluids
- Cross reactivity:Human TMEM132D ELISA kit exhibits high specificity and excellent specificity for the detection of human TMEM132D. No significant cross-reactivity or interference between TMEM132D and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf life:Store for 6 months at 4 °C
- Detection range:0,156 - 10 ng/ml
- Storage temperature:4 °C
- Sample volume:100 μl
- Sensitivity:0,094 ng/ml
- Regulatory status:RUO
Human TMEM132D ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human TMEM132D in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-to-use ELISA kit
- Detection range: 0,156 to 10 ng/ml
- Sensitivity: 0,094 ng/ml
- Sample type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Human TMEM132D ELISA kit (A74563) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human TMEM132D in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for TMEM132D has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the TMEM132D present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-TMEM132D Antibody, which binds the captured TMEM132D present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of TMEM132D captured in each well. The concentration of TMEM132D can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.