Advanced
Order Entry
Northern Ireland
ContactUsLinkComponent
 
Sheep Ghrelin ELISA Kit
  ANTIA75112-96
 :  ANTIBODIES.COM
New Product
 :  
undefined
Sheep Ghrelin ELISA Kit
  ANTIA75112-96
 :  ANTIBODIES.COM
New Product
 :  

Some Products May Appear Restricted

To ensure a smooth and speedy checkout, please log in to your account. Some items may show as restricted simply because you're not logged in.

If you do not have an account, you can register using our registration webform (https://www.avantorsciences.com/us/en/login/register)

 

If you're still seeing restrictions after logging in, certain products—like chemicals or medical devices—require additional account verification steps to be able to place an order. Some items may additionally require a specific license or customer documentation;  additional documentation will be requested for these items prior to shipment. 

 

  • Pk:
    96 Tests
  • Assay duration:
    Multiple steps
  • Assay Type:
    Competitive
  • Format:
    Pre-coated
  • Primary antibody reactivity:
    Other
  • Target protein:
    Ghrelin
  • Description:
    Sheep Ghrelin ELISA kit
  • Sample type:
    Serum, plasma, tissue homogenates and other biological fluids
  • Cross reactivity:
    Sheep Ghrelin ELISA Kit exhibits high specificity and excellent specificity for the detection of sheep Ghrelin. No significant cross-reactivity or interference between Ghrelin and analogues was observed.
  • Detection method:
    Colorimetric
  • Time to Results:
    4 h 30 min
  • Assay Principle:
    Quantitative
  • Shelf life:
    Store for 6 months at 4 °C
  • Detection range:
    3,125 - 200 pg/ml
  • Storage temperature:
    4 °C
  • Sample volume:
    50 μl
  • Sensitivity:
    1,875 pg/ml
  • Regulatory status:
    RUO

 

 

Sheep Ghrelin ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of sheep Ghrelin in serum, plasma, tissue homogenates, and other biological fluids.

  • Ready-to-use ELISA kit
  • Detection range: 3,125 to 200 pg/ml
  • Sensitivity: 1,875 pg/ml
  • Sample type: Serum, plasma, tissue homogenates, and other biological fluids
  • Assay Precision: Intra to Assay: CV <8%, Inter to Assay: CV <10%

Sheep Ghrelin ELISA Kit (A75112) employs the competitive enzyme immunoassay technique for the quantitative measurement of sheep Ghrelin in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with Ghrelin antigen. During the incubation, Ghrelin present in the samples or standards competes with the fixed amount of immobilised Ghrelin for binding sites on the Biotinylated Anti-Ghrelin Antibody. The more Ghrelin present in a sample or standard, the less Biotinylated Anti-Ghrelin Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Ghrelin Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Ghrelin present in each sample or standard. The concentration of Ghrelin can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

 : This is a ready-to-use ELISA kit that contains: Pre-coated 96 well microplate, lyophilised standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-Streptavidin conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.