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Specifications
- Pk:96 Tests
- Assay duration:Multiple steps
- Assay Type:Sandwich
- Format:Pre-coated
- Host:
- Primary antibody reactivity:Human
- Target protein:NMDAR2A
- Description:Human NMDAR2A ELISA kit
- Environmentally Preferable:
- Sample type:Serum, plasma, tissue homogenates and other biological fluids
- Cross reactivity:Human NMDAR2A ELISA Kit exhibits high specificity and excellent specificity for the detection of human NMDAR2A. No significant cross-reactivity or interference between NMDAR2A and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf life:Store for 6 months at 4 °C
- Detection range:0,313 - 20 ng/ml
- Storage temperature:4 °C
- Sample volume:100 μl
- Sensitivity:0,188 ng/ml
- Regulatory status:RUO
Specifications
About this item
Human NMDAR2A ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human NMDAR2A in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-to-use ELISA kit
- Detection range: 0,313 to 20 ng/ml
- Sensitivity: 0,188 ng/ml
- Sample type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra to Assay: CV <8%, Inter to Assay: CV <10%
Human NMDAR2A ELISA Kit (A74807) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human NMDAR2A in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for NMDAR2A has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the NMDAR2A present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-NMDAR2A Antibody, which binds the captured NMDAR2A present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of NMDAR2A captured in each well. The concentration of NMDAR2A can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.