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- Pk:96 Tests
- Assay duration:Multiple steps
- Assay Type:Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Mouse
- Target protein:Ccl21a
- Description:Mouse Ccl21a ELISA kit
- Sample type:Serum, plasma, tissue homogenates and other biological fluids
- Cross reactivity:Mouse Ccl21a ELISA kit exhibits high specificity and excellent specificity for the detection of mouse Ccl21a. No significant cross-reactivity or interference between Ccl21a and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf life:Store for 6 months at 4 °C
- Detection range:15,625 - 1000 pg/ml
- Storage temperature:4 °C
- Sample volume:100 μl
- Sensitivity:9,375 pg/ml
- Regulatory status:RUO
Mouse Ccl21a ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse Ccl21a in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-To-Use ELISA Kit
- Detection Range: 15,625 - 1000 pg/ml
- Sensitivity: 9,375 pg/ml
- Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Mouse Ccl21a ELISA kit (A78789) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse Ccl21a in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Ccl21a has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Ccl21a present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Ccl21a Antibody, which binds the captured Ccl21a present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Ccl21a captured in each well. The concentration of Ccl21a can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.