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Specifications
- Pk:96 Tests
- Assay duration:Multiple steps
- Assay Type:Competitive
- Format:Pre-coated
- Primary antibody reactivity:Rat
- Target protein:Corticosterone
- Description:Rat corticosterone ELISA kit
- Sample type:Serum, plasma, tissue homogenates and other biological fluids
- Cross reactivity:Rat Corticosterone ELISA kit exhibits high specificity and excellent specificity for the detection of rat Corticosterone. No significant cross-reactivity or interference between Corticosterone and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf life:Store for 6 months at 4 °C
- Detection range:2,813 - 180 ng/ml
- Storage temperature:4 °C
- Sample volume:50 μl
- Sensitivity:1,688 ng/ml
- Regulatory status:RUO
Specifications
About this item
Rat Corticosterone ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of rat Corticosterone in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-To-Use ELISA Kit
- Detection Range: 2,813 - 180 ng/ml
- Sensitivity: 1,688 ng/ml
- Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Rat Corticosterone ELISA kit (A79228) employs the competitive enzyme immunoassay technique for the quantitative measurement of rat Corticosterone in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with Corticosterone antigen. During the incubation, Corticosterone present in the samples or standards competes with the fixed amount of immobilized Corticosterone for binding sites on the Biotinylated Anti-Corticosterone Antibody. The more Corticosterone present in a sample or standard, the less Biotinylated Anti-Corticosterone Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Corticosterone Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Corticosterone present in each sample or standard. The concentration of Corticosterone can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.