Some Products May Appear Restricted
To ensure a smooth and speedy checkout, please log in to your account. Some items may show as restricted simply because you're not logged in.
If you do not have an account, you can register using our registration webform (https://www.avantorsciences.com/us/en/login/register)
If you're still seeing restrictions after logging in, certain products—like chemicals or medical devices—require additional account verification steps to be able to place an order. Some items may additionally require a specific license or customer documentation; additional documentation will be requested for these items prior to shipment.
Specifications
- Pk:96 Tests
- Assay duration:Multiple steps
- Assay Type:Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Rat
- Target protein:Smad3
- Description:Rat Smad3 ELISA kit
- Sample type:Serum, plasma, tissue homogenates and other biological fluids
- Cross reactivity:Rat Smad3 ELISA Kit exhibits high specificity and excellent specificity for the detection of rat Smad3. No significant cross-reactivity or interference between Smad3 and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf life:Store for 6 months at 4 °C
- Detection range:0,156 - 10 ng/ml
- Storage temperature:4 °C
- Sample volume:100 μl
- Sensitivity:0,094 ng/ml
- Regulatory status:RUO
Specifications
About this item
Rat Smad3 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat Smad3 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready To Use ELISA Kit
- Detection Range: 0,156 to 10 ng/ml
- Sensitivity: 0,094 ng/ml
- Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%
Rat Smad3 ELISA Kit (A78800) employs the sandwich enzyme immunoassay technique for the quantitative measurement of rat Smad3 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Smad3 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Smad3 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Smad3 Antibody, which binds the captured Smad3 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Smad3 captured in each well. The concentration of Smad3 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.