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- Pk:96 Tests
- Assay duration:Multiple steps
- Assay Type:Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Rat
- Target protein:S100A4
- Description:Rat S100A4 ELISA kit
- Sample type:Serum, plasma, tissue homogenates and other biological fluids
- Cross reactivity:Rat S100A4 ELISA kit exhibits high specificity and excellent specificity for the detection of rat S100A4. No significant cross-reactivity or interference between S100A4 and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf life:Store for 6 months at 4 °C
- Detection range:7,813 - 500 pg/ml
- Storage temperature:4 °C
- Sample volume:100 μl
- Sensitivity:4,688 pg/ml
- Regulatory status:RUO
Rat S100A4 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat S100A4 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-To-Use ELISA Kit
- Detection Range: 7,813 - 500 pg/ml
- Sensitivity: 4,688 pg/ml
- Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Rat S100A4 ELISA kit (A78759) employs the sandwich enzyme immunoassay technique for the quantitative measurement of rat S100A4 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for S100A4 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the S100A4 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-S100A4 Antibody, which binds the captured S100A4 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of S100A4 captured in each well. The concentration of S100A4 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.